BRASSINOSTEROID‐SIGNALING KINASE 1 phosphorylating CALCIUM/CALMODULIN‐DEPENDENT PROTEIN KINASE functions in drought tolerance in maize

Liu, Lei; Xiang, Yang; Yan, Jingwei; Di, Pengcheng; Li, Jing; Sun, Xiujuan; Han, Gaoqiang; Ni, Lan; Jiang, Mingyi; Yuan, Jianhua; Zhang, Aying

doi:10.1111/nph.17403

Cited by 38 publications

(32 citation statements)

References 78 publications

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“…To generate transgenic plants, full‐length ZmMPK5 and ZmSOD3 were amplified using specific primers (Supporting Information Table S1) and inserted into the binary vector pCUN‐NHF with Flag/HA‐tag driven by the maize ubiquitin promoter using a ClonExpressII One Step Cloning Kit (Vazyme, Nanjing, China). For knockdown transgenic plants, a fragment containing two reverse complementary fragments joined by a 400‐bp GUS intron (Table S1) was inserted into the binary vector pCUN‐NHF driven by the maize ubiquitin promoter (Liu et al ., 2021). The recombinant vectors were transformed into maize by shoot‐tip transformation using Agrobacterium tumefaciens strain LBA4404 (Liu et al ., 2015; Ma et al ., 2018; Xiang et al ., 2021).…”

Section: Methodsmentioning

confidence: 99%

ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize

et al. 2021

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Mitogen-activated protein kinase (MPK) is a critical regulator of the antioxidant defence system in response to various stimuli. However, how MPK directly and exactly regulates antioxidant enzyme activities is still unclear. Here, we demonstrated that a NAC transcription factor ZmNAC49 mediated the regulation of antioxidant enzyme activities by ZmMPK5.ZmNAC49 expression is induced by oxidative stress. ZmNAC49 enhances oxidative stress tolerance in maize, and it also reduces superoxide anion generation and increases superoxide dismutase (SOD) activity. A detailed study showed that ZmMPK5 directly interacts with and phosphorylates ZmNAC49 in vitro and in vivo. ZmMPK5 directly phosphorylates Thr-26 in NAC subdomain A of ZmNAC49. Mutation at Thr-26 of ZmNAC49 does not affect the interaction with ZmMPK5 and its subcellular localisation.Further analysis found that ZmNAC49 activates the ZmSOD3 expression by directly binding to its promoter. ZmMPK5-mediated ZmNAC49 phosphorylation improves its ability to bind to the ZmSOD3 promoter. Thr-26 of ZmNAC49 is essential for its transcriptional activity. In addition, ZmSOD3 enhances oxidative stress tolerance in maize.Our results show that phosphorylation of Thr-26 in ZmNAC49 by ZmMPK5 increased its DNA-binding activity to the ZmSOD3 promoter, enhanced SOD activity and thereby improved oxidative stress tolerance in maize.

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Section: Methodsmentioning

confidence: 99%

ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize

et al. 2021

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“…Ca 2+ not only acts as a secondary messenger in the rapid response to upstream stimulation, but more importantly, the Ca 2+ signaling system also contains a large number of different types of calcium signal receptors, such as CDPKs, CaM, CBL, and CIPK, which receive exogenous calcium signals and convert them into endogenous calcium signals. These signals are then phosphorylated and dephosphorylated or eventually interact with other proteins to regulate stomatal movement [ 34 , 35 , 36 ]. The interaction between CBL9 and CIPK3 negatively regulates Ca 2+ -dependent ABA signaling in Arabidopsis [ 37 ].…”

Section: Molecular Mechanisms Of Crosstalk Between Ca 2+ ...mentioning

confidence: 99%

Crosstalk between Ca2+ and Other Regulators Assists Plants in Responding to Abiotic Stress

Liu

Jin

et al. 2022

Plants

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Plants have evolved many strategies for adaptation to extreme environments. Ca2+, acting as an important secondary messenger in plant cells, is a signaling molecule involved in plants’ response and adaptation to external stress. In plant cells, almost all kinds of abiotic stresses are able to raise cytosolic Ca2+ levels, and the spatiotemporal distribution of this molecule in distant cells suggests that Ca2+ may be a universal signal regulating different kinds of abiotic stress. Ca2+ is used to sense and transduce various stress signals through its downstream calcium-binding proteins, thereby inducing a series of biochemical reactions to adapt to or resist various stresses. This review summarizes the roles and molecular mechanisms of cytosolic Ca2+ in response to abiotic stresses such as drought, high salinity, ultraviolet light, heavy metals, waterlogging, extreme temperature and wounding. Furthermore, we focused on the crosstalk between Ca2+ and other signaling molecules in plants suffering from extreme environmental stress.

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“…AtBSKs [11, 17-32, 40, 41], ve rice OsBSKs [15,16], two woodland tobacco NsBSKs (i.e. NsBSK1 and NsBSK3) [42], a common tobacco NtBSK2 [42], and nine maize ZmBSKs [35] have been reported…”

Section: Phylogenetic Relationship Of Bsks From Spinach and Other Pla...mentioning

confidence: 99%

“…Besides, the expressional patterns of some members of BSKs were investigated in wild barley [36], Kentucky bluegrass [37], and P. tomentosa [38] in response to drought and cold, respectively. However, only a few of BSKs were cloned and characterized in plants, such as AtBSK1, AtBSK2, AtBSK3, and AtBSK5 in Arabidopsis [11, 18, 21-30, 33, 40], OsBSK1-2 and OsBSK3 in rice [15,16], NsBSK1 and NsBSK3 in woodland tobacco [42], NtBSK2 in common tobacco [42], and ZmBSK1 in maize [35]. The phylogenetic and functional analyses of BSKs from green vegetables, such as in spinach, have not been conducted so far.…”

Section: The Sobsks Had Close Homology With Sugar Beet Bvbsksmentioning

confidence: 99%

“…Besides of Arabidopsis, several BSKs from other plants were reported to function in diverse biological processes, such as the sprouting of potato (Solanum tuberosum) [34], drought response of maize (Zea mays) [35], wild barley (Hordeum spontaneum) [36] and Kentucky bluegrass (Poa pratensis) [37], cold tolerance of Populus tomentosa [38], as well as immune response of rice (Oryza sativa) [15] and tomato (Solanum lycopersicum) [39]. However, the BSK-regulated mechanisms in these courses need to be further studied.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification and expression analysis reveals spinach brassinosteroid-signaling kinase (BSK) gene family functions in temperature stress response

Yang

Zhang

et al. 2022

Preprint

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Background: Brassinosteroid-signaling kinase (BSK) is a critical family of receptor-like cytoplasmic kinase for BR signal transduction, which plays important roles in the development, immunity-/ abiotic stress- response, and hormone regulation of plants. Spinach (Spinacia oleracea) is cold tolerant but heat sensitive green leafy vegetable. A comprehensive study on the mechanisms of BSK family members and BSK- mediated stress response in spinach have not been performed.Results: We identified and cloned seven SoBSKs in spinach. Phylogenetic and collinearity analyses suggested that SoBSKs had close relationship with dicotyledonous sugar beet (Beta vulgaris) rather than monocotyledons. The gene structure, and conserved protein domain/ motif analyses indicated that most SoBSKs were relative conserved, while SoBSK6 could be a truncated member. The predicted functional post-translation modification (PTM) sites in SoBSKs suggested their probable roles in signal transduction, redox regulation, and protein turnover of SoBSKs, and the N-terminal myristoylation site in SoBSKs was critical for their localization to cell periphery. Cis-acting elements for light, drought, temperature (heat and cold), and hormone response distributed wildly in the promoters of SoBSKs, implying the pivotal roles of SoBSKs in response to diverse abiotic stress and phytohormone stimuli. Most SoBSKs were highly expressed in leaves, except of SoBSK7 in roots. Many SoBSKs were differentially regulated by heat stress, cold stress, as well exogenous BL and ABA treatments. The bsk134678 mutant seedlings exhibited more heat tolerance than wild-type and SoBSK1-overexpressed seedlings.Conclusions: A comprehensive genome- wide analysis of SoBSK in spinach presented a global identification and functional prediction of SoBSKs. Seven SoBSKs had relative conserved gene structure and protein function domains. Except for SoBSK6, all the other SoBSKs had the similar motifs and conserved modification sites. Most SoBSKs participated in the temperature and hormone (BR and ABA) responses. These findings paved the way for further functional analyses on BSK- mediated mechanism in spinach development and stress responses.

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BRASSINOSTEROID‐SIGNALING KINASE 1 phosphorylating CALCIUM/CALMODULIN‐DEPENDENT PROTEIN KINASE functions in drought tolerance in maize

Cited by 38 publications

References 78 publications

ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize

ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize

Crosstalk between Ca2+ and Other Regulators Assists Plants in Responding to Abiotic Stress

Genome-wide identification and expression analysis reveals spinach brassinosteroid-signaling kinase (BSK) gene family functions in temperature stress response

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