BRASSINOSTEROID INSENSITIVE1 internalization can occur independent of ligand binding

Claus, Lucas Alves Neubus; Liu, Derui; Hohmann, Ulrich; Vukašinović, Nemanja; Pleskot, Roman; Liu, Jing; Schiffner, Alexei; Jaillais, Yvon; Wu, Gwo-Jang; Wolf, Sebastián; Damme, Daniël Van; Hothorn, Michael; Russinova, Eugenia

doi:10.1093/plphys/kiad005

Cited by 7 publications

(2 citation statements)

References 52 publications

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“…Indeed, BRI1 has been reported to have functions independent of BRs (Holzwart et al, 2020). Moreover BRI1 internalization can occur independently of its binding to the hormone (Neubus Claus et al, 2023). In the future it will be interesting to elucidate how BRI1 mediates H3K27me3 removal, and how this pathway can be uncoupled from BRs.…”

Section: Discussionmentioning

confidence: 99%

BRI1-mediated removal of seed coat H3K27me3 marks is a brassinosteroid-independent process

Pankaj,

Lima,

Luo

et al. 2023

Preprint

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Seed development in flowering plants starts with a double fertilization event where two paternal gametes, the sperm cells, fertilize their maternal counterparts, egg cell and central cell. This leads to the formation of the embryo and the endosperm. These fertilization products are enveloped by the maternally-derived seed coat, the development of which is inhibited prior to fertilization by epigenetic regulator Polycomb Repressive Complex 2 (PRC2). This complex deposits the repressive histone mark H3K27me3, whose removal is necessary for seed coat formation. However, H3K27me3 marks are stable and PRC2 removal does not explain how seed coat genes become activated after fertilization. Here, we show that JUMONJI-type (JMJ) histone demethylases are expressed in the seed coats ofArabidopsis thaliana(Arabidopsis) and are necessary for its formation. We further propose that JMJ activity is coupled to Brassinosteroid (BR) function, as BR effectors have been shown to physically recruit JMJ proteins to target loci. Consistent with this hypothesis, we show that loss of BR function leads to seed coat defects, which can be rescued by depletion of H3K27me3. Finally, we reveal an additional pathway through which BRs directly regulate seed coat development, independently of H3K27me3 deposition. This discovery highlights the diverse functions of BRs in coordinating seed development, beyond their known roles in plant growth and development.

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Section: Discussionmentioning

confidence: 99%

BRI1-mediated removal of seed coat H3K27me3 marks is a brassinosteroid-independent process

Pankaj,

Lima,

Luo

et al. 2023

Preprint

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“…Plant hormones are biologically active compounds produced by their cells, which have a relatively complex structure and affect certain chemical molecules in the process of their metabolism [1]. In recent years, research on the role of the representatives of a rather large class of steroid hormones, brassinosteroids, in the regulation of cell metabolism at the level of signaling systems has been developing at a rather rapid rate [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Influence of Exogenous 24-Epicasterone on the Hormonal Status of Soybean Plants

Derevyanchuk,

Kretynin,

Bukhonska

et al. 2023

Plants

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Brassinosteroids (BRs) are key phytohormones involved in the regulation of major processes of cell metabolism that guide plant growth. In the past decades, new evidence has made it clear that BRs also play a key role in the orchestration of plant responses to many abiotic and biotic stresses. In the present work, we analyzed the impact of foliar treatment with 24-epicastasterone (ECS) on the endogenous content of major phytohormones (auxins, salicylic acid, jasmonic acid, and abscisic acid) and their intermediates in soybean leaves 7 days following the treatment. Changes in the endogenous content of phytohormones have been identified and quantified by LC/MS. The obtained results point to a clear role of ECS in the upregulation of auxin content (indole-3-acetic acid, IAA) and downregulation of salicylic, jasmonic, and abscisic acid levels. These data confirm that under optimal conditions, ECS in tested concentrations of 0.25 µM and 1 µM might promote growth in soybeans by inducing auxin contents. Benzoic acid (a precursor of salicylic acid (SA)), but not SA itself, has also been highly accumulated under ECS treatment, which indicates an activation of the adaptation strategies of cell metabolism to possible environmental challenges.

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