Brassicasterol from Edible Aquacultural Hippocampus abdominalis Exerts an Anti-Cancer Effect by Dual-Targeting AKT and AR Signaling in Prostate Cancer

Xu, Yinzhu; Ryu, Soo-In; Lee, You-Kyung; Lee, Hyo‐Jeong

doi:10.3390/biomedicines8090370

Cited by 11 publications

(12 citation statements)

References 47 publications

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“…AKT and ERK pathways are the common pathways involving tumorigenesis and targeting these pathways are proven to be effective against several cancers [ 23 , 24 ]. In addition, previous study has shown that brassicasterol inhibited prostate cancer growth by targeting AKT pathway [ 19 ]. To investigate if brassicasterol suppressed the growth of HBV + HCC through downregulation of one or both pathways, western blot was performed.…”

Section: Resultsmentioning

confidence: 99%

Brassicasterol inhibits hepatitis B virus-associated hepatocellular carcinoma development via suppression of AKT signaling pathway

Zeng

Pang

et al. 2023

Infect Agents Cancer

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Background Hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) does not respond well to current treatment options like sorafenib, and there is an urgent need for developing therapeutical strategies for HBV + HCC. Brassicasterol has previously shown anti-cancer and anti-viral activities, however, its value against HBV + HCC remains to be explored. Methods The inhibitory effect of brassicasterol and sorafenib was evaluated on HBV + HCC cell lines and xenograft mouse model. The cytotoxicity of brassicasterol on normal liver cells were measured by LDH assay. AKT agonist was used to identify the targeted signaling pathway by brassicasterol. Results Brassicasterol induced HBV + HCC cell death in a both dose-dependent and time-dependent manner, and such inhibition was more potent than sorafenib. Brassicasterol did not show apparent cytotoxicity to normal liver cells. Xenograft mouse model further confirmed the inhibitory effect of brassicasterol on the growth of HBV + HCC. Furthermore, signaling pathway analysis showed that brassicasterol-treated HBV + HCC cells had decreased level of phosphor-AKT expression while the addition of AKT agonist could counteract the inhibitory effect of brassicasterol on HCC, indicating that brassicasterol suppressed AKT pathway to exhibit anti-cancer activity in HBV + HCC cells. In addition, brassicasterol showed similar levels of inhibition on HBV− and HBV + HCC cells. Conclusion Brassicasterol possesses anti-cancer activity against HCC through the downregulation of AKT pathway and such activity is independent of HBV infection.

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Section: Resultsmentioning

confidence: 99%

Brassicasterol inhibits hepatitis B virus-associated hepatocellular carcinoma development via suppression of AKT signaling pathway

Zeng

Pang

et al. 2023

Infect Agents Cancer

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“…The expression of PARP was inhibited by brassicasterol treatment, during which induced cleaved caspase-3 expression was noted. Brassicasterol inhibits cell migration in LNCaP cells, as well [ 125 ]. Furthermore, it inhibited n-butyl-(4-hydroxybutyl) nitrosamine (BHBN) and SS-induced carcinogenesis.…”

Section: Health Benefits and Biomedical Applications Of Phytosterolsmentioning

confidence: 99%

Phytosterols in Seaweeds: An Overview on Biosynthesis to Biomedical Applications

Sohn

Rathinapriya

Balaji

et al. 2021

IJMS

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Seaweed extracts are considered effective therapeutic alternatives to synthetic anticancer, antioxidant, and antimicrobial agents, owing to their availability, low cost, greater efficacy, eco-friendliness, and non-toxic nature. Since the bioactive constituents of seaweed, in particular, phytosterols, possess plenty of medicinal benefits over other conventional pharmaceutical agents, they have been extensively evaluated for many years. Fortunately, recent advances in phytosterol-based research have begun to unravel the evidence concerning these important processes and to endow the field with the understanding and identification of the potential contributions of seaweed-steroidal molecules that can be used as chemotherapeutic drugs. Despite the myriad of research interests in phytosterols, there is an immense need to fill the void with an up-to-date literature survey elucidating their biosynthesis, pharmacological effects, and other biomedical applications. Hence, in the present review, we summarize studies dealing with several types of seaweed to provide a comprehensive overview of the structural determination of several phytosterol molecules, their properties, biosynthetic pathways, and mechanisms of action, along with their health benefits, which could significantly contribute to the development of novel drugs and functional foods.

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“…In the present investigation, the brassicasterol, 24-methylene-cholesterol, 22dehydrocholesterol, and isofucosterol were recorded as the dominant NCSs. Brassicasterolrich lipids from edible Hippocampus abdominalis have shown cytotoxicity against LNCaP human prostate cancer cells [50]. Lipid fractions rich in 22-dehydrocholesterol, brassicasterol and other marine animal specific sterols from the Persian Gulf sponge, Axinella sinoxea, have also shown cytotoxicity against human lymphoblastic leukemia (MOLT-4), human breast adenocarcinoma (MCF-7), and human colorectal adenocarcinoma (HT-29) cells.…”

Section: Anticancer Potential Of Lipids Extracted From Shellfish Speciesmentioning

confidence: 99%

Anticancer Potential of Lipophilic Constituents of Eleven Shellfish Species Commonly Consumed in Korea

Shin

Song

et al. 2021

Antioxidants

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The present study was aimed to investigate the composition and contents and the major lipophilic compounds, including the sterols, fatty acids, and tocols of shellfish species. Moreover, to explore the antitumor activity of these lipophilic constituents, their cytotoxicity potentials were determined against five different human cancer cells, including colon carcinoma (HCT116), epithelial melanoma (A2058), glioblastoma multiforme (T98G), lung carcinoma (A549), and adenocarcinoma (HeLa). The results show a significant variation in the contents and composition of lipophilic constituents among the studied species. The highest omega-3 (n-3) polyunsaturated fatty acids (PUFAs) were recorded from arrow squid and pacific oysters, accounting for 53.2% and 53.0% of their total fatty acids, respectively. However, the highest cholesterol content was also recorded in arrow squid (154.4 mg/100 g; 92.6% of total sterols). In contrast, in the Japanese littleneck, Yesso scallop, and common orient clam, cholesterol was just 17.1%, 18.3%, and 18.9% of total sterols, respectively, making them the richest source of non-cholesterol sterols (NCS). Lipids extracted from shellfish species showed ABTS+•- and DPPH•-scavenging activities. In the cytotoxicity analysis, lipids extracted from the Argentine red shrimp showed the highest cytotoxicity against glioblastoma multiforme T98G cells, with an IC50 value of 12.3 µg/mL. The composition and cytotoxicity data reported herein may help explore the nutritional and anticancer potentials of shellfish species.

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Brassicasterol from Edible Aquacultural Hippocampus abdominalis Exerts an Anti-Cancer Effect by Dual-Targeting AKT and AR Signaling in Prostate Cancer

Cited by 11 publications

References 47 publications

Brassicasterol inhibits hepatitis B virus-associated hepatocellular carcinoma development via suppression of AKT signaling pathway

Brassicasterol inhibits hepatitis B virus-associated hepatocellular carcinoma development via suppression of AKT signaling pathway

Phytosterols in Seaweeds: An Overview on Biosynthesis to Biomedical Applications

Anticancer Potential of Lipophilic Constituents of Eleven Shellfish Species Commonly Consumed in Korea

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