Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production

Pelletier, Georges; Budar, Françoise

doi:10.1007/978-1-4939-2389-2_9

Cited by 11 publications

(7 citation statements)

References 77 publications

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“…Protoplasts from a normal B. napus line were fused with protoplasts from a CMS (Ogura radish cytoplasm) B. napus, and protoplasts from a normal B. oleracea line were fused with protoplasts from a CMS (Ogura radish cytoplasm) B. oleracea, in order to select cybrids carrying only Brassica chloroplasts that grew normally. These improved CMS lines are known as Ogu-INRA and are widely used to produce hybrids in Brassicaceae (Pelletier and Budar, 2015). Similar advances have been achieved by Indian breeders who developed and characterized several Ogu-CMS lines in cauliflower and cabbage (Dey et al, 2017a;Bathia et al, 2015;Parkash et al, 2015).…”

Section: Brassicaceasmentioning

confidence: 86%

Genotypic Diversity in 291 Maize Lines From Cimmyt and Phenotypic Characterization in Southern Cordoba, Argentina

Rossi

Ruiz

Renzo

et al. 2019

BAG

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CIMMYT maize inbred lines (CMLs) are freely distributed to breeding programs around the world. Better information on phenotypic and genotypic diversity may provide guidance to breeders on how to use more efficiently the CMLs in their breeding programs. In this study a group of 291 CIMMYT maize inbred lines, was phenotyped by nine agro-morphological traits in south Córdoba, Argentina and genotyped using 18,082 SNPs. Based on the geographic information and the environmental adaptation, 291 CMLs were classified into eight subgroups. Anthesis-silking interval (IAE) was the trait with higher phenotypic diversity. A 40% of maize inbred lines, with IAE less than five days, show a good adaptation to growing conditions in south Córdoba, Argentina. The low phenotypic variation explained by environmental adaptation subgroups indicates that population structure is only a minor factor contributing to phenotypic diversity in this panel. Principal component analysis (ACP) allowed us to obtain phenotypic and genotypic orderings. Generalized procrustes analysis (APG) indicated a 60% consensus between both data type from the total panel of maize lines. In each environmental adaptation subgroup, the APG consensus was higher. This result, which might indicate linkage disequilibrium between SNPs markers and the genes controlling these agro-morphological traits, is promising and could be used as an initial tool in the identification of Quantitative Trait Loci (QTL). Information on genetic diversity, population structure and phenotypic diversity in local environments will help maize breeders to better understand how to use the current CIMMYT maize inbred lines group. Key words: broad-sense heritability, multivariate analysis, SNPs, agro-morphological traits.

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Section: Brassicaceasmentioning

confidence: 86%

Genotypic Diversity in 291 Maize Lines From Cimmyt and Phenotypic Characterization in Southern Cordoba, Argentina

Rossi

Ruiz

Renzo

et al. 2019

BAG

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“…). These improved CMS lines are known as Ogu‐INRA and are widely used to produce hybrids in Brassicaceae (Pelletier and Budar , Kaminski et al. ).…”

Section: Pollination Control Methods In Different Vegetable Cropsmentioning

confidence: 99%

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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Production of hybrid varieties of vegetable crops is currently a desired breeding goal, due to their remarkable agronomic performance and to the possibility of intellectual property protection. However, efficient hybrid production requires a careful pollination control to guarantee the hybrid nature of F 1 seed. Several technologies ranging from manual emasculation to genetic transformation are used to inhibit pollen production in mother plants. In this review, we examine the principles underlying strategies like genetically determined systems (genic male sterility, cytoplasmic-genic male sterility, self-incompatibility) and other methods (manual emasculation, chemical-hybridizing agents) in different species, considering the benefits and drawbacks of their adoption. Finally, we present the current state of the art for vegetable hybrid seed production.

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“…Ces résultats rejoignent en partie les observations de Mesquida et al (1991) et de Pierre et al (1999) réalisées sur les volumes de nectar sécrétés par des lignées pures MF et MS avec la mesure du taux de sécrétion apparent. Cette différence de vitesse de sécrétion peut s'expliquer en partie par la mise en place du système de stérilité mâle cytoplasmique : lors de la conception de ce système, les premières lignées MS obtenues en combinant le génome nucléaire du colza avec le cytoplasme du radis Raphanus sativus ont eu pour effet d'altérer le développement des nectaires (Pelletier et Budar, 2015).…”

Section: La Lignée Ms Sécrète Deux Fois Moins Vite Que La Lignée F1unclassified

Mesurer la sécrétion nectarifère : exemple d'une lignée hybride F1 et de son parent mâle stérile chez le colza d'hiver (Brassica napusL.)

Chabert¹,

Lemoine²,

Fronteau³

et al. 2017

OCL

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Reçu le 5 octobre 2017 -Accepté le 7 novembre 2017Résumé -Au cours de l'histoire évolutive des plantes à fleurs, l'apparition des nectaires floraux a permis de substituer le pollen par du nectar pour attirer les animaux pollinisateurs, permettant de diminuer les coûts de la pollinisation animale liés à la consommation du pollen. Dans les productions de semence hybride des cultures entomophiles, connaître le niveau de sécrétion nectarifère des lignées en présence, mâle fertile (MF) et mâle stérile (MS), est important si l'on souhaite maximiser les transferts de pollen entre les deux. Dans cet article, nous faisons tout d'abord une revue des méthodes qui existent pour mesurer la sécrétion nectarifère, puis retenons celle qui mesure un taux de sécrétion brut, qui permet d'exprimer une vitesse de sécrétion, pour l'utiliser sur deux lignées de colza d'hiver (Brassica napus L.), la variété hybride F1 Exocet MF et son parent MS. Nous montrons que la sécrétion nectarifère du colza est constante sur un intervalle de temps de 6-8 heures durant les heures du jour, que cette sécrétion admet une température optimale se situant entre 20°C et 30°C, et qu'elle est autour de deux fois moindre chez le parent MS par rapport à la lignée F1. Ces résultats permettent de proposer une méthode de mesure rigoureuse pour comparer la sécrétion nectarifère entre lignées ou variétés. Nous concluons sur les principales autres variables dont il faudrait tenir compte pour notamment pouvoir estimer la quantité totale de nectar sécrétée par une surface donnée de culture.Mots clés : méthode / sécrétion nectarifère / colza / semence hybride / température Abstract -Measuring nectar secretion: the example of an F1 hybrid and its male sterile parent in winter oilseed rape (Brassica napus L.). During the evolutionary history of flowering plants, the appearance of floral nectaries allowed the replacement of pollen by nectar to attract pollinators, allowing lower costs involved in animal pollination by reducing the consumption of pollen. In the hybrid seed productions of entomophilous crops, knowing the levels of nectar secretion of the different lines, the male fertile (MF) and the male sterile (MS) ones, is important to maximize pollen transfers between them. In this study, we start with a review of current methods used to measure nectar secretion, and choose the one that provides a gross secretion rate in order to use it on two winter oilseed rape (Brassica napus L.) lines, the hybrid F1 'Exocet' and its MS parent. We show that oilseed rape has a gross nectar secretion rate that is constant over a period of 6-8 hours during daylight hours, that it has a thermal optimum included between 20°C and 30°C, and that the parental MS line secretes about half as much as the hybrid F1 one. These results enable us to propose a rigorous method to compare nectar secretions between lines and varieties. We conclude with the main other variables that should be taken into account to estimate the total amount of nectar produced by a given area of crop.

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Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production

Cited by 11 publications

References 77 publications

Genotypic Diversity in 291 Maize Lines From Cimmyt and Phenotypic Characterization in Southern Cordoba, Argentina

Genotypic Diversity in 291 Maize Lines From Cimmyt and Phenotypic Characterization in Southern Cordoba, Argentina

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Mesurer la sécrétion nectarifère : exemple d'une lignée hybride F1 et de son parent mâle stérile chez le colza d'hiver (Brassica napusL.)

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