Brassica napus miR169 regulates BnaNF-YA in salinity, drought and ABA responses

Wang, Ji; Zhou, Zhaosheng; Qing, Tao; Chen, Xiuping; Shui, Chen; Ren, Xuyang; Yu, Li; Liang, Mingxiang

doi:10.1016/j.envexpbot.2022.104882

Cited by 13 publications

(7 citation statements)

References 63 publications

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“…), rape ( Brassica napus ), and potato ( Solanum tuberosum ; Yang et al, 2016 ) crops. For example, in response to drought and ABA treatment, Bna-miR169n targets BnaNF-YA9 in Brassica napus ( Wang et al, 2022 ). PtmiR169o targets the PtNF-YA6 gene to regulate drought tolerance in poplar ( Jiao et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng

Fan

Sun²,

Zhuo³

et al. 2022

Front. Plant Sci.

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Agropyron mongolicum (A. mongolicum) is an excellent gramineous forage with extreme drought tolerance, which lives in arid and semiarid desert areas. However, the mechanism that underlies the response of microRNAs (miRNAs) and their targets in A. mongolicum to drought stress is not well understood. In this study, we analyzed the transcriptome, small RNAome (specifically the miRNAome) and degradome to generate a comprehensive resource that focused on identifying key regulatory miRNA-target circuits under drought stress. The most extended transcript in each collection is known as the UniGene, and a total of 41,792 UniGenes and 1,104 miRNAs were identified, and 99 differentially expressed miRNAs negatively regulated 1,474 differentially expressed target genes. Among them, eight miRNAs were unique to A. mongolicum, and there were 36 target genes. A weighted gene co-expression network analysis identified five hub genes. The miRNAs of five hub genes were screened with an integration analysis of the degradome and sRNAs, such as osa-miR444a-3p.2-MADS47, bdi-miR408-5p_1ss19TA-CCX1, tae-miR9774_L-2R-1_1ss11GT-carC, ata-miR169a-3p-PAO2, and bdi-miR528-p3_2ss15TG20CA-HOX24. The functional annotations revealed that they were involved in mediating the brassinosteroid signal pathway, transporting and exchanging sodium and potassium ions and regulating the oxidation–reduction process, hydrolase activity, plant response to water deprivation, abscisic acid (ABA) and the ABA-activated signaling pathway to regulate drought stress. Five hub genes were discovered, which could play central roles in the regulation of drought-responsive genes. These results show that the combined analysis of miRNA, the transcriptome and degradation group provides a useful platform to investigate the molecular mechanism of drought resistance in A. mongolicum and could provide new insights into the genetic engineering of Poaceae crops in the future.

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Section: Discussionmentioning

confidence: 99%

Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng

Fan

Sun²,

Zhuo³

et al. 2022

Front. Plant Sci.

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“…NFYA , targeted by miR169, has been validated in maize, soybean, and oilseed rape. In these species, miR169 expression are repressed by various stresses such as salt and drought; whereas the opposite pattern is observed for NFYA genes ( Luan et al., 2014 ; Yu et al., 2019 ; Wang et al., 2022 ). Our present study validated that HcNFYA1 is a true target of miR169b in H. caspica by RLM-RCE technology ( Figure 1A ).…”

Section: Discussionmentioning

confidence: 90%

“…In plants, regulatory modules composed of miRNAs and their target genes play important roles in physiological and biochemical processes ( Song et al., 2019 ; Samad et al., 2017 ; Pagano et al, 2021 ). miR169 is one of the largest miRNA families in plants, and its target genes mainly encode the transcription factor nuclear factor Y-A (NFYA) ( Luan et al., 2014 ; Chaves-Sanjuan et al., 2021 ; Wang et al., 2022 ). NFYA proteins form trimers with NFYB/C and then bind to CCAAT elements in downstream gene promoters to influence gene expression ( Laloum et al., 2013 ; Zanetti et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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The miR169b/NFYA1 module from the halophyte Halostachys caspica endows salt and drought tolerance in Arabidopsis through multi-pathways

Zeng²,

Zhang³

et al. 2023

Front. Plant Sci.

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Salt and drought are the major abiotic stress factors plaguing plant growth, development and crop yields. Certain abiotic-stress tolerant plants have developed special mechanisms for adapting to adverse environments in the long process of evolution. Elucidating the molecular mechanisms by which they can exert resistance to abiotic stresses is beneficial for breeding new cultivars to guide agricultural production. Halostachys caspica, a perennial halophyte belonging to Halostachys in Amaranthaceae, is extremely tolerant to harsh environments, which is commonly grown in the saline-alkali arid desert area of Northwest, China. However, the molecular mechanism of stress tolerance is unclear. Nuclear Factor Y-A (NFYA) is a transcription factor that regulates the expression of downstream genes in plant response to adverse environments. It has also been reported that some members of the NFYA family are the main targets of miR169 in plants. In this study, we mainly focused on exploring the functions and preliminary mechanism of the miR169b/NFYA1 module from H. caspica to abiotic stress. The main results showed that RLM-RACE technology validated that HcNFYA1 was targeted by HcmiR169b, qRT-PCR revealed that HcmiR169b was repressed and HcNFYA1 was induced in the H. caspica branches under various abiotic stress as well ABA treatment and Arabidopsis stable transformation platform with molecular methods was applied to elucidate that the HcmiR169b/HcNFYA1 module conferred the salt and drought tolerance to plants by enhancing ABA synthesis and ABA signal transduction pathways, maintaining ROS homeostasis and the stability of cell membrane. HcNFYA1 is expected to be a candidate gene to improve plant resistance to salt and drought stresses.

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“…The miR169 family members have significant roles in plant abiotic and biotic stress responses and are involved in various aspects of plant development, including root architecture and nodule formation, by targeting Nuclear Factor‐YA ( NF‐YA ) gene family members (Aslam et al., 2020; Li et al., 2017, 2021; Ni et al., 2013; Sorin et al., 2014; Wang et al., 2022; Zhang, 2015; Zhao et al., 2011). There are four mature miR169 isoforms in the model plant A. thaliana which are encoded by 14 MIR169 genomic loci (Li et al., 2010), while there are eight miR169 family members annotated from 18 precursor loci in tomato (Rao et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

A conserved HSF:miR169:NF‐YA loop involved in tomato and Arabidopsis heat stress tolerance

et al. 2022

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Heat stress transcription factors (HSFs) and microRNAs (miRNAs) regulate different stress and developmental networks in plants. Regulatory feedback mechanisms are at the basis of these networks. Here, we report that plants improve their heat stress tolerance through HSF-mediated transcriptional regulation of MIR169 and post-transcriptional regulation of Nuclear Factor-YA (NF-YA) transcription factors. We show that HSFs recognize tomato (Solanum lycopersicum) and Arabidopsis MIR169 promoters using yeast one-hybrid/ chromatin immunoprecipitation-quantitative PCR. Silencing tomato HSFs using virus-induced gene silencing (VIGS) reduced Sly-MIR169 levels and enhanced Sly-NF-YA9/A10 target expression. Further, Sly-NF-YA9/A10 VIGS knockdown tomato plants and Arabidopsis plants overexpressing At-MIR169d or At-nf-ya2 mutants showed a link with increased heat tolerance. In contrast, Arabidopsis plants overexpressing At-NF-YA2 and those expressing a non-cleavable At-NF-YA2 form (miR169d-resistant At-NF-YA2) as well as plants in which At-miR169d regulation is inhibited (miR169d mimic plants) were more sensitive to heat stress, highlighting NF-YA as a negative regulator of heat tolerance. Furthermore, post-transcriptional cleavage of NF-YA by elevated miR169 levels resulted in alleviation of the repression of the heat stress effector HSFA7 in tomato and Arabidopsis, revealing a retroactive control of HSFs by the miR169:NF-YA node. Hence, a regulatory feedback loop involving HSFs, miR169s and NF-YAs plays a critical role in the regulation of the heat stress response in tomato and Arabidopsis plants.

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Brassica napus miR169 regulates BnaNF-YA in salinity, drought and ABA responses

Cited by 13 publications

References 63 publications

Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng

Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng

The miR169b/NFYA1 module from the halophyte Halostachys caspica endows salt and drought tolerance in Arabidopsis through multi-pathways

A conserved HSF:miR169:NF‐YA loop involved in tomato and Arabidopsis heat stress tolerance

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