Branched peptide with three histidines for the promotion of Cu^II binding in a wide pH range – complementary potentiometric, spectroscopic and electrochemical studies

Abstract

Potentiometric, spectroscopic (UV-Vis, CD and EPR), mass spectrometric and electrochemical data indicate that in monomeric Cu II -3H complexes the metal is bound with higher affinity compared to its structural domains indicating that the effect of 3D branching should be taken as an important factor for future studies on Cu II peptide constructs.

Tuning the coordination properties of multi-histidine peptides by using a tripodal scaffold: solution chemical study and catechol oxidase mimicking

“…26 Copper(II) complexes of some histidine-containing branched peptides have also been studied. 27,28 Surprisingly, oligonuclear complexes were not detected in spite of the high number of available donor sites.…”

“…Only a few reports are available in the literature describing metal ion interactions with tripodal peptides, [17][18][19][20][21][22][23][24][25][26][27][28] most of them are related to sulphur-containing ligands. [17][18][19][20][21] A series of tricarboxylic acids have been used 22 …”

“…Several tripodal peptides with different platforms have been designed as receptors of either organic molecules 16 or metal ions. [17][18][19][20][21][22][23][24][25][26][27][28][29] These ligands have several advantages in addition to the pre-organized structure and enhanced chelate effect. Using different amino acids, it is relatively easy to fine-tune the metal ion binding ability.…”

Armed by Asp? C-terminal carboxylate in a Dap-branched peptide and consequences in the binding of CuII and electrocatalytic water oxidation

“…SWV settings were: 0.15 mM Cu-2GD, 100 mM NaClO 4 electrolyte, 25 C, pulse width 20 ms (f ¼ 25 Hz), step potential 0.4 mV, SW pulse height 25 mV. The raw current curves (net current, i net ¼ forward minus reverse current, i for À i rev ) were baseline corrected 33,52,56 to obtain the curves plotted in Fig. 5.…”

“…The working electrode was carefully rinsed, polished, ultrasonicated and rinsed again before use. 52 The cell was kept under argon throughout the measurements, the O 2 level was checked with a uorescent O 2 probe (Ocean Optics NeoFox). SWV settings were: 0.15 mM Cu-2GD, 100 mM NaClO 4 electrolyte, 25 C, pulse width 20 ms (f ¼ 25 Hz), step potential 0.4 mV, SW pulse height 25 mV.…”