Brain-wide monosynaptic connectivity mapping with ROInet-seq
Zhige Lin,
Osnat Ophir,
Thorsten Trimbuch
et al.
Abstract:Viral projection tracing strategies help establish regional connectomes of mammalian brains. Monosynaptic connectivity tracing with G-deleted rabies virus (RV) establishes retrograde synaptic connectivity, but cannot distinguish networks at cell resolution. We developed ROInet-seq, an accessible spatial method using barcoded LambdaG rabies virus, amenable to readout by RNA-sequencing. We optimized library complexity and uniformity, such that detection of specific barcodes can reliably distinguish multiple infe… Show more
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