Brain-targeting gene delivery and cellular internalization mechanisms for modified rabies virus glycoprotein RVG29 nanoparticles

Liu, Yang; Huang, Rongqin; Han, Liang; Ke, Weilun; Shao, Kun; Ye, Liya; Lou, Jinning; Jiang, Chen

doi:10.1016/j.biomaterials.2009.02.051

Cited by 223 publications

(155 citation statements)

References 34 publications

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“…The authors then showed the ability of the RVG peptide to noninvasively confer protection via the intravenous delivery of a siRNA targeted against fatal flaviviral encephalitis, wherein the peptide-delivered construct mediated 80% survival compared with controls. Based on the knowledge that the RVG motif could mediate the crossing of the BBB, Liu et al conjugated the RVG peptide to PEGylated PAMAM dendrimers that were subsequently self-assembled with plasmid DNA encoding create PEG/PAMAM/DNA NPs [125]. Upon intravenous injection, the NPs concentrated in the mouse brain within 80 min ( FiguRe 6C).…”

Section: Neuropeptidementioning

confidence: 99%

“…A thorough characterization of the resulting in vitro and in vivo toxicities is clearly necessary for each newly generated NP-peptide assembly. Over time, studies have evolved from those wherein cells are incubated with rather high NP-peptide concentrations (e.g., 1 µM QDs decorated with mito chondrial-signal peptides [115]) with little regard to cellular toxicity to those in which the minimal amount of NP-peptide conjugate Reproduced with permission from [116,125] © American Chemical Society.…”

Section: Toxicity Of Peptide-delivered Npsmentioning

confidence: 99%

“…High drug-loading capacity and controllable cargo release [73,91,98,125] plasma membrane-derived vesicles. This delivery approach has been used for the labeling of numerous cell lines with a variety of NP materials including nanodiamonds [9], QDs [10] and Au-NPs [11].…”

Section: Cellular Delivery Of Nanoparticlesmentioning

confidence: 99%

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Peptides for Specific Intracellular Delivery and Targeting of Nanoparticles: Implications for Developing nanoparticle-mediated Drug Delivery

Delehanty¹,

Boeneman

Bradburne

et al. 2010

Therapeutic Delivery

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The use of peptides to mediate the delivery and uptake of nanoparticle (NP) materials by mammalian cells has grown significantly over the past 10 years. This area of research has important implications for the development of new therapeutic materials and for the emerging field of NP-mediated drug delivery. In this review, we highlight recent advances in the delivery of various NPs by some of the more commonly employed cellular delivery peptides and discuss important related factors such as NP-peptide bioconjugation, uptake efficiency, intracellular fate and toxicity. We also highlight various demonstrations of therapeutic applications of NP-peptide conjugates where appropriate. The paper concludes with a brief forward-looking perspective discussing what can be expected as this field develops in the coming years.

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Section: Neuropeptidementioning

confidence: 99%

Section: Toxicity Of Peptide-delivered Npsmentioning

confidence: 99%

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Peptides for Specific Intracellular Delivery and Targeting of Nanoparticles: Implications for Developing nanoparticle-mediated Drug Delivery

Delehanty¹,

Boeneman

Bradburne

et al. 2010

Therapeutic Delivery

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show abstract

“…Like most drugs, nanocarriers show low permeability at the blood-brain barrier. Thus, targeting ligands, such as transferrin, angiopep peptide, 4 apolipoprotein, 6 rabies virus glycoprotein peptide, 7 and phage display peptide, 8 have been used to modify the surface of nanocarriers to increase the efficiency of uptake by the brain. However, there remain issues of uptake efficiency, especially in the brain parenchyma.…”

Section: Introductionmentioning

confidence: 99%

Intracellular delivery mechanism and brain delivery kinetics of biodegradable cationic bovine serum albumin-conjugated polymersomes

Pang¹,

Gao²,

Shen³

et al. 2012

IJN

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Background: A novel brain drug delivery system using cationic bovine serum albumin (CBSA)-conjugated biodegradable polymersomes (CBSA-PO) was prepared, and its intracellular delivery mechanism and brain delivery kinetics were evaluated. Methods and results: Biodegradable poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) was used to prepare the polymersomes, and thiolated CBSA was conjugated with the surface of the polymersome. Transmission electron microscopy and dynamic light scattering showed that the CBSA-PO had a round and vesicle-like shape, with a mean diameter of around 100 nm. Coupling of CBSA with polymersomes was confirmed by X-ray photoelectron spectroscopy. Uptake of CBSA-PO by bEnd.3 cells was significantly higher than that of unconjugated polymersomes, but was inhibited by low temperature, free CBSA, and poly-L-lysine, indicating that endocytosis was energy-driven and absorptive-mediated. Cell viability assays confirmed the good safety profile of biodegradable CBSA-PO. Pharmacokinetic results demonstrated that the polymersomes had long circulation times, and CBSA conjugation on the polymersomes significantly increased the blood-brain barrier permeability surface area product by 3.6-fold and the percentage of injected dose per gram brain (% ID/g brain) by 2.1-fold. Capillary depletion experiments showed that CBSA-PO was distributed into the brain parenchyma in a time-dependent manner, with few polymersomes detected, indicating that conjugation of polymersomes with CBSA significantly improved their transcytosis across the brain-blood barrier. Conclusion: These results suggest that CBSA-PO is a promising drug brain delivery carrier with low toxicity.

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“…The same situation occurred with mesenchymal stem cells transfected with CP-pDNA nanoparticles/propidium iodide added with colchicine (lower panels in Figure 6). Colchicine is a widely used endocytosis inhibitor 43,44 that can effectively inhibit endocytosis in a cell membrane, as shown by the significantly reduced red fluorescence observed in the lower panels of Figure 6. The increasing intensive red fluorescence in the CP-pDNA nanoparticle group indicated that an increasing amount of plasmid DNA was transported into the cytoplasm.…”

mentioning

confidence: 99%

Encapsulation of plasmid DNA in calcium phosphate nanoparticles: stem cell uptake and gene transfer efficiency

Cao¹,

Deng²,

Yuan³

et al. 2011

IJN

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Background The purpose of this study was to develop calcium phosphate nanocomposite particles encapsulating plasmid DNA (CP-pDNA) nanoparticles as a nonviral vector for gene delivery. Methods CP-pDNA nanoparticles employing plasmid transforming growth factor beta 1 (TGF-β1) were prepared and characterized. The transfection efficiency and cell viability of the CP-pDNA nanoparticles were evaluated in mesenchymal stem cells, which were identified by immunofluorescence staining. Cytotoxicity of plasmid TGF-β1 and calcium phosphate to mesenchymal stem cells were evaluated by MTT assay. Results The integrity of TGF-β1 encapsulated in the CP-pDNA nanoparticles was maintained. The well dispersed CP-pDNA nanoparticles exhibited an ultralow particle size (20–50 nm) and significantly lower cytotoxicity than Lipofectamine™ 2000. Immunofluorescence staining revealed that the cultured cells in this study were probably mesenchymal stem cells. The cellular uptake and transfection efficiency of the CP-pDNA nanoparticles into the mesenchymal stem cells were higher than that of needle-like calcium phosphate nanoparticles and a standard calcium phosphate transfection kit. Furthermore, live cell imaging and confocal laser microscopy vividly showed the transportation process of the CP-pDNA nanoparticles in mesenchymal stem cells. The results of a cytotoxicity assay found that both plasmid TGF-β1 and calcium phosphate were not toxic to mesenchymal stem cells. Conclusion CP-pDNA nanoparticles can be developed into an effective alternative as a nonviral gene delivery system that is highly efficient and has low cytotoxicity.

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Brain-targeting gene delivery and cellular internalization mechanisms for modified rabies virus glycoprotein RVG29 nanoparticles

Cited by 223 publications

References 34 publications

Peptides for Specific Intracellular Delivery and Targeting of Nanoparticles: Implications for Developing nanoparticle-mediated Drug Delivery

Peptides for Specific Intracellular Delivery and Targeting of Nanoparticles: Implications for Developing nanoparticle-mediated Drug Delivery

Intracellular delivery mechanism and brain delivery kinetics of biodegradable cationic bovine serum albumin-conjugated polymersomes

Encapsulation of plasmid DNA in calcium phosphate nanoparticles: stem cell uptake and gene transfer efficiency

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