Brain stem slice conditioned medium contains endogenous BDNF and GDNF that affect neural crest boundary cap cells in co-culture

Cochlear nucleus whole mount explants promote the differentiation of neuronal stem cells from the cochlear nucleus in co-culture experiments

“…The level of this factor was slightly lower compared to the level found in the supernatant of auditory brainstem slices. However the composition of the basal medium was different in these studies (Kaiser et al, 2014). Interestingly, at 2 days in the culture without added whole-mounts and NSCs the level of BDNF was significantly higher compared to the other groups.…”

“…This was presumably caused by the degradation of the growth factors in the medium. Previous studies also showed low levels of BDNF in serum free medium after 8 days in culture (Kaiser et al, 2014). Levels of NT-3 were also analysed in all investigated groups and were found not to be different from the basal media (Fig.…”

“…Several transplantation and co-culture studies of exogenous stem cells have been performed in the auditory brainstem displaying good integration of the cells (GlavaskiJoksimovic et al, 2008;Jiao et al, 2011;Kaiser et al, 2014;Novozhilova et al, 2013). However since the cochlear nucleus is located near the fourth ventricle, neurosurgical approaches are difficult and only performed in the rare case of vestibular schwannoma surgery and/or auditory brainstem implantation (Matthies et al, 2013).…”

Evaluation of established human iPSC-derived neurons to model neurodegenerative diseases

“…In order to improve maturation properties, we cultured the neurons in iCell Ò medium conditioned by rat C6 glioma cells up to 35 days. It has been shown that conditioned medium can improve the properties of cells in a relatively short time frame (Unsicker et al, 1984;Rushton et al, 2013;Kaiser et al, 2014). These results provide further evidence into how secreted glial cell factors can lead to improved maturation of induced pluripotent stem cell-derived neurons.…”

“…A thorough characterization by microarray, immunocytochemistry and functional calcium imaging is performed. As several studies have shown that conditioned medium can improve neuronal differentiation and maturation (Unsicker et al, 1984;Liu et al, 2013;Kaiser et al, 2014), cell culture medium was conditioned with rat glioma cell medium, in order to improve the functional and maturation properties of the cells. Last, the potential for gene overexpression is assessed using a viral approach.…”