Brain Reaction to Experimental Injury After Hypothermia

Rosomoff, Hubert L.; Clasen, Raymond A.; Hartstock, Robert; Bebin, José

doi:10.1001/archneur.1965.00470040003001

Cited by 33 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Temperature has also shown to be a critical variable in TBI and acute inflammation studies, with hypothermia affording cerebral protection and reducing neutrophil migration Rosomoff et al, 1965;. In our study, we maintained all subjects at normothermia during FPI or sham procedures (measured by rectal thermometer).…”

Section: Discussionmentioning

confidence: 99%

P-Selectin Blockade Following Fluid-Percussion Injury: Behavioral and Immunochemical Sequelae

Grady

Cody²,

Maris³

et al. 1999

Journal of Neurotrauma

View full text Add to dashboard Cite

Traumatic brain injury (TBI) can cause polymorphonuclear leukocyte (PMN) migration into brain parenchyma, mediating various cytodestructive mechanisms. We examined the effect of blocking leukocyte/endothelial cell adhesion molecules (CAMs) on the anatomic and behavioral sequelae in lateral fluid-percussion injury in rats. Monoclonal antibodies (MAb) directed against a functional (PB1.3) or nonfunctional (PNB1.6) epitope on endothelial P-selectin were used as treatments. Subjects were tested in the Morris water maze (MWM) at 7 and 14 days postinjury then immunohistochemistry was performed using antibodies that recognize ChAT, GFAP and OX-42. A second set of animals underwent myeloperoxidase (MPO) assay in the brain parenchyma and a third set was used to examine neutrophil migration using the MAb RP-3. Time in quadrant, but not escape latency or proximity improved with PB1.3 (p < 0.05). Similarly, PB1.3 reduced MPO levels after injury (p < 0.05), in the ipsilateral cortex. No significant difference occurred in neutrophil counts in cortex, corpus callosum, hippocampus, and thalamus between injured only rats and injured rats treated with PB1.3. Quantitative analysis of cholinergic cells in the medial septum showed a protective effect by PB1.3. Densitometry readings of GFAP and OX-42 immunolabeling revealed no discernible differences between the treated and untreated injured rats. Qualitatively, there was no difference in microglia or astrocyte response to treatment. Treatment with P-selectin blockade in brain-injured rats may reduce PMN migration into brain, help preserve cholinergic immunolabeling of medial septal nucleus neurons, and may alleviate mnemonic deficits.

show abstract

Section: Discussionmentioning

confidence: 99%

P-Selectin Blockade Following Fluid-Percussion Injury: Behavioral and Immunochemical Sequelae

Grady

Cody²,

Maris³

et al. 1999

Journal of Neurotrauma

View full text Add to dashboard Cite

show abstract

“…In models of focal ischemia, strategies that deplete neutrophils or prevent adhesion to endothelial cells reduced histologie damage and improved functional outcome (Shiga et al, 1991;Matsuo et al, 1994;Zhang et al, 1995;Connelly et al, 1996). Rosomoff et al (1965) noted neutrophil accumulation in brain early after freeze injury and suggested that hypothermia mitigated secondary injury from inflammation in this model.…”

mentioning

confidence: 90%

The Effect of Brain Temperature on Acute Inflammation after Traumatic Brain Injury in Rats

Whalen

Carlos²,

Clark³

et al. 1997

Journal of Neurotrauma

View full text Add to dashboard Cite

The effect of varying brain temperature on neutrophil accumulation in brain and the expression of E-selectin and intercellular adhesion molecule-1 (ICAM-1) on cerebrovascular endothelium after controlled cortical impact (CCI) was studied in rats. Sprague Dawley rats were anesthetized and subjected to CCI to the left parietal cortex. Ten minutes after CCI, brain temperature was modulated and maintained at 32 degrees C, 37 degrees C, or 39 degrees C (n = 8 per group) for 4 h. Rats were then decapitated and immunohistochemistry on brain sections was performed using monoclonal antibodies (MoAb) that recognize neutrophils (RP-3), ICAM-1 (TM-8, Athena Neurosciences), or MoAb that react with E-selectin (La-Roche). Each of these markers was quantified in 100 x fields. Neutrophil accumulation was also quantified with myeloperoxidase (MPO) assay. Absolute neutrophil count (ANC) was measured in blood samples before and 1 h and 4 h after CCI. Neutrophil accumulation in injured brain was decreased in rats maintained at 32 degrees C vs 39 degrees C (4-fold difference as assessed by immunohistochemistry, p < 0.05; 8-fold difference as assessed by MPO assay, p < 0.05). Peripheral blood ANC was not affected by temperature. E-selectin was induced on cerebrovascular endothelium after CCI (p < 0.05), but was only decreased modestly at 32 degrees C versus 39 degrees C (p = 0.11). ICAM-1 was not upregulated on cerebrovascular endothelium at this early time following CCI. Neutrophil accumulation is directly dependent on brain temperature during the initial 4 h after CCI. This appears to be mediated by mechanisms other than effects of temperature on E-selectin or ICAM-1 expression or systemic ANC.

show abstract

“…Treatment includes steroids, aspirin, and other anti-inflammatories to inhibit pain and neurologic deficit. Focal physical forms of therapy, such as the applying of ice, may reverse pathologic changes, and thus limit or avert tissue reaction to injury [18]. Vigorous activation of the musculoskeletal system can lead to changes in central nervous system chemistry, eg, enkephalin release, and favorably affect the endogenous pain control system.…”

Section: Physical Medicine and Rehabilitationmentioning

confidence: 99%

“…Nonetheless, scientific rationale exists for some modalities. Ice application with temperature lowering is known to decrease nerve conduction to the point of anesthesia, and inflammatory reaction is contained with a reduction of chronic changes [18]. To be effective, the area must be packed in ice for longer than 30 minutes.…”

Section: Management Regimensmentioning

confidence: 99%

“…These chemical reactions are inhibited by steroids, aspirin, and related products, effects that then provide a rationale for pharmacologic treatment [17]. However, even more interesting is the reversal of these same phenomena by focal physical forms of therapy such as application of ice, which, parenthetically, has been well established as a method for limiting or averting tissue reaction to injury [18]. Vigorous activation of the musculoskeletal system also limits the reaction to injury, because enkephalins are released at two to three times the baseline levels.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Surgery for the herniated lumbar disk with nerve root entrapment: Are alternative treatments to surgical intervention effective?

Rosomoff

1998

Current Review of Pain

View full text Add to dashboard Cite

Brain Reaction to Experimental Injury After Hypothermia

Cited by 33 publications

References 13 publications

P-Selectin Blockade Following Fluid-Percussion Injury: Behavioral and Immunochemical Sequelae

P-Selectin Blockade Following Fluid-Percussion Injury: Behavioral and Immunochemical Sequelae

The Effect of Brain Temperature on Acute Inflammation after Traumatic Brain Injury in Rats

Surgery for the herniated lumbar disk with nerve root entrapment: Are alternative treatments to surgical intervention effective?

Contact Info

Product

Resources

About