Brain microvascular endothelial cells derived from human induced pluripotent stem cells as in vitro model for assessing blood-brain barrier transferrin receptor-mediated transcytosis

Development of a three-dimensional blood-brain barrier network with opening capillary structures for drug transport screening assays

“…We thus sought to investigate if our model could reproduce this reduced paracellular transport in vitro . The TEER value measured in our 3D model was about 560 ​Ω x cm 2 , which is higher than the acellular fibrin gel which has a TEER of about 51 ​Ω x cm 2 ( Figure S5 ) and the previously reported TEER value of 2D HBEC monolayer of about 20 ​Ω x cm 2 [ 42 ]. Additionally, the paracellular marker Lucifer Yellow showed an apparent permeability coefficient value (P app ) and effective permeability coefficient value (P e ) of 8.51 ​× ​10 −7 ​cm/s and 4.17 ​× ​10 −6 ​cm/s respectively ( Fig.…”

“…HiPS-BMEC have attracted increasing attention in BBB modelling due to their ability to reproduce several BBB features, including physiologically relevant TEER, high expression of BMEC specific transporters and efflux pumps, and size-selective transport of molecules [ 13 , 63 , 64 ]. We recently reported hiPS-BMEC showed higher expression of some TJs and TfR, as well as a lower paracellular transport as compared to HBEC [ 42 ]. It could be interesting to know if hiPS-BMEC could form the same perfusable network with open structures than the one observed with HBEC in the fibrin gel.…”

Apicobasal transferrin receptor localization and trafficking in brain capillary endothelial cells

“…Noteworthy for the plethora of studies within TfR and the BBB, the majority have considered only apical TfR localization in order to accept the system as accessible for brain drug delivery, and convincingly reported TfR expression in BECs across numerous species and models [ 2 , 4 , 30 , 31 ]. Investigations of basal TfR expression, however, remain limited [ 32 ], despite previous studies suggesting TfR to be altered apically, intracellularly, and basally in BECs, dependent on the brain iron status [ 20 ].…”

P450 oxidoreductase regulates barrier maturation by mediating retinoic acid metabolism in a model of the human BBB

“…iBMECs are increasingly used to model various aspects of the BBB ( Motallebnejad et al., 2022 ; Ohshima et al., 2019 ; Park et al., 2019 ; Piantino et al., 2022 ; Rapier et al., 2022 ; Vatine et al., 2019 ). Their strength lies in their scalability and functional properties and the opportunity to model disorders in a personalized manner ( Lim et al., 2017 ; Vatine et al., 2017 ; Wu et al., 2021 ).…”