Brain fatty acid binding protein exhibits non-preferential and mutation-resistant binding towards fatty acids

Bodnariuc, Iulia; Lenz, Stefan A. P.; Renaud-Young, Margaret; Shandro, Tanille M.; Ishida, Hiroaki; Vogel, Hans J.; MacCallum, Justin L.

doi:10.1101/2021.09.23.461245

Cited by 3 publications

(2 citation statements)

References 47 publications

(87 reference statements)

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“…With the network-based ingredient screening model, we highlighted four major ingredients (GABA, stearic acid, tetradecanoic acid, and coumestrol) of ZSSG that might be able to regulate sleep quality. Taking stearic acid as an example, experimental research has demonstrated that this ingredient binds to FBAP7 [ 50 ]. FBAP7 knock-out mice displayed an increase in wake during the light phase, indicating that FBAP7 is required for normal sleep inhibition [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Integrating animal experiments, mass spectrometry and network-based approach to reveal the sleep-improving effects of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Ren,

Wu,

Wang

et al. 2023

Chin Med

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Background Ziziphi Spinosae Semen (ZSS) is a plant widely used as medicine and food in Asian countries due to its numerous health benefits. γ-aminobutyric acid (GABA), a non-proteinaceous amino acid, is one of the major inhibitory neurotransmitters with a relaxant function. In this study, a system pharmacology approach was employed to assess the effects of a mixture composed of ZSS and GABA (ZSSG) on sleep improvement. Methods Mice were divided into five groups (n = 10) and received either no treatment, sodium pentobarbital, or sodium barbital with diazepam or ZSSG. The effects of ZSSG on sleep quality were evaluated in mice, and differential metabolites associated with sleep were identified among the control, ZSS, GABA, and ZSSG groups. Additionally, network-based ingredient-insomnia proximity analysis was applied to explore the major ingredients. Results ZSSG significantly improved sleep quality by decreasing sleep latency and prolonging sleep duration in sodium pentobarbital-induced sleeping mouse model (P < 0.05). ZSSG significantly enhanced the brain content of GABA in mice. Furthermore, ZSSG also significantly decreased sleep latency-induced by sodium barbital in mice (P < 0.05). Metabolic analysis revealed significant differences in 10 metabolites between ZSSG group and the groups administering ZSS or GABA. Lastly, using the network-based ingredient screening model, we discovered potential four active ingredients and three pairwise ingredient combinations with synergistic effect on insomnia from ZSSG among 85 ingredients identified by UPLC-Q/TOF–MS. Also, we have constructed an online computation platform. Conclusion Our data demonstrated that ZSSG improved the sleeping quality of mice and helped to balance metabolic disorders-associated with sleep disorders. Moreover, based on the network-based prediction method, the four potential active ingredients in ZSSG could serve as quality markers-associated with insomnia. The network-based framework may open up a new avenue for the discovery of active ingredients of herbal medicine for treating complex chronic diseases or symptoms, such as insomnia.

show abstract

Section: Discussionmentioning

confidence: 99%

Integrating animal experiments, mass spectrometry and network-based approach to reveal the sleep-improving effects of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Ren,

Wu,

Wang

et al. 2023

Chin Med

View full text Add to dashboard Cite

show abstract

“…With the network-based ingredient screening model, we highlighted four major ingredients (GABA, stearic acid, tetradecanoic acid, and coumestrol) of ZSSG that might be able to regulate sleep quality. Taking stearic acid as an example, experimental research has demonstrated that this ingredient binds to FBAP7 [44]. FBAP7 knock-out mice displayed an increase in wake during the light phase, indicating that FBAP7 is required for normal sleep inhibition [45].…”

Section: Discussionmentioning

confidence: 99%

Integrating animal experiment, mass spectrometry and network-based approach to reveal the improving sleep quality of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Ren

Wang

et al. 2022

Preprint

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Background Ziziphi Spinosae Semen (ZSS), which is widely consumed as a medicine and food dual-use plant in some regions of Asian countries, has various health benefits. γ-aminobutyric acid (GABA), a non-proteinaceous amino acid, is one of the major neurotransmitter inhibitors that has a relaxant function. In this study, a system pharmacology approach was proposed to evaluate the effects of a mixture composed by ZSS and GABA (ZSSG) on sleep improvement. Methods Mice were divided into 5 groups (n = 10) and received either no treatment or sodium pentobarbital or sodium barbital with Diaz or ZSSG. The effects of ZSSG on improving sleep quality were evaluated in mice. The major ingredients of ZSSG were analyzed by UPLC-Q/TOF-MS. Network-based ingredient-insomnia proximity was applied to explore its major ingredients. Results The results showed that ZSSG displayed significant effects on decreasing sleep latency and prolonging sleep duration in sodium pentobarbital-induced sleeping mouse model (P < 0.05). ZSSG significantly enhanced the brain content of GABA in mice. In addition, ZSSG also significantly decreased sleep latency-induced by sodium barbital in mice (P < 0.05). With the network-based ingredient screening model, four active ingredients and three pairwise ingredient combinations were discovered from ZSSG among 85 ingredients in ZSSG identified by UPLC-Q/TOF-MS. Conclusion These data demonstrated that ZSSG improved the sleeping quality of mice. The potential four active ingredients and three pairwise ingredient combinations could serve as quality markers associated with insomnia. The network-based framework may open up a new avenue for the discovery of active ingredients of herbal medicine for treating complex chronic diseases, such as insomnia.

show abstract

Integrating animal experiments, mass spectrometry and network-based approach to reveal the improving sleep quality of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Cui

Ren

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Ziziphi Spinosae Semen (ZSS) is a plant widely used as medicine and food in Asian countries due to its numerous health benefits. γ-aminobutyric acid (GABA), a non-proteinaceous amino acid, is one of the major inhibitory neurotransmitters with a relaxant function. In this study, a systems pharmacology approach was employed to assess the effects of a mixture composed of ZSS and GABA (ZSSG) on sleep improvement.Methods Mice were divided into five groups (n = 10) and received either no treatment, sodium pentobarbital, or sodium barbital with diazepam or ZSSG. The effects of ZSSG on sleep quality were evaluated in mice, and differential metabolites associated with sleep were identified among the control, ZSS, GABA, and ZSSG groups. Additionally, a network-based ingredient-insomnia proximity analysis was applied to explore the major ingredients.Results ZSSG significantly improved sleep quality by decreasing sleep latency and prolonging sleep duration in sodium pentobarbital-induced sleeping mouse model (P < 0.05). ZSSG significantly enhanced the brain content of GABA in mice. Furthermore, ZSSG also significantly decreased sleep latency-induced by sodium barbital in mice (P < 0.05). Metabolic analysis revealed significant differences in 10 metabolites between ZSSG group and the groups administering ZSS or GABA. Lastly, using the network-based ingredient screening model, we discovered potential four active ingredients and three pairwise ingredient combinations with synergistic effect for insomnia from ZSSG among 85 ingredients identified by UPLC-Q/TOF-MS. Also, we have constructed an online computation platform.Conclusion Our data demonstrated that ZSSG improved the sleeping quality of mice and help to balance metabolic disorders-associated with sleep disorders. Moreover, based on the network-based prediction method, the potential four active ingredients in ZSSG could serve as quality markers-associated with insomnia. The network-based framework may open up a new avenue for the discovery of active ingredients of herbal medicine for treating complex chronic diseases or symptom, such as insomnia.

show abstract

Brain fatty acid binding protein exhibits non-preferential and mutation-resistant binding towards fatty acids

Cited by 3 publications

References 47 publications

Integrating animal experiments, mass spectrometry and network-based approach to reveal the sleep-improving effects of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Integrating animal experiments, mass spectrometry and network-based approach to reveal the sleep-improving effects of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Integrating animal experiment, mass spectrometry and network-based approach to reveal the improving sleep quality of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Integrating animal experiments, mass spectrometry and network-based approach to reveal the improving sleep quality of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

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