Brain Fatty Acid-binding Protein and ω-3/ω-6 Fatty Acids

Mita, Raja; Beaulieu, Michaël; Field, Catherine J.; Godbout, Roseline

doi:10.1074/jbc.m110.170076

Cited by 93 publications

(64 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FABP5 RT-PCR products could be generated in low levels from U251 cell RNA, in the presence or absence of RA treatment, but the FABP5 protein was undetectable (Figure 3A and 3B). These results are supported by a previous study [24]. DNA sequencing confirmed that the 281 bp RT-PCR product was amplified with FABP5 primers and was specifically generated from FABP5 transcripts, which encompassed Exon 1 to Exon 3 (data not shown).…”

Section: Resultssupporting

confidence: 90%

CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid

Xia

et al. 2015

Oncotarget

View full text Add to dashboard Cite

Glioblastomas respond differently to all-trans retinoic acid (RA) for unknown reasons. Because CRABP-II and FABP5 mediate RA intracellular signaling respectively and lead to distinct biological consequences, their expression patterns in different grades of astrocytomas and the glioblastoma cells lines LN18, LN428 and U251 were examined to identify potential correlations with RA sensitivities. The response of glioblastoma cells to RA, decitabine or the FABP5 competitive inhibitor, BMS309403, was analyzed. CRABP-II and FABP5 were expressed to varying degrees by the 84-astrocytoma cases examined. Treatment of LN428, U251 and LN18 cells with RA failed to suppress their growth; however, U251 proliferation was inhibited by decitabine. The combination of decitabine and RA suppressed the growth of all three cell lines and induced significant apoptosis of LN428 and U251 cells. Both CRABP-II and FABP5 were transcribed in the three cell lines but FABP5 proteins were undetectable in U251 cells. The ratio of CRABP-II to FABP5 was not altered after RA, decitabine or RA and decitabine treatment and the resistance of cells to RA was not reversed by BMS309403 treatment. In conclusion, CRABP-II and FABP5 expression patterns are neither related to the tumor grades nor correlated with RA sensitivity. Additional molecular factors may be present that determines the sensitivity of glioblastoma cells to RA. Dicitabine may improve the sensitivity of glioblastoma cells to RA, however, its underlying mechanism and its in vivo feasibility need to be investigated.

show abstract

Section: Resultssupporting

confidence: 90%

CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid

Xia

et al. 2015

Oncotarget

View full text Add to dashboard Cite

show abstract

“…The mRNA of Fabp7, mainly detected in brain, was included in our study because this member of the Fabp family was occasionally detected in the zebrafish (Danio rerio) testis (Liu et al 2003) and because it is involved in the transport of the 22-carbon PUFA-like docosahexaenoic acid (Balendiran et al 2000, Mita et al 2010. This fatty acid, like docosapentaenoic acid, abounds in mouse testis lipids (Furland et al 2003), where it is highly concentrated in membrane GPLs and TAGs, of germ cells and RB (present results).…”

Section: Discussionmentioning

confidence: 99%

Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases, and perilipin 2

Oresti¹,

García-López²,

Aveldaño³

et al. 2013

Reproduction

View full text Add to dashboard Cite

Male germ cell differentiation entails the synthesis and remodeling of membrane polar lipids and the formation of triacylglycerols (TAGs). This requires fatty acid-binding proteins (FABPs) for intracellular fatty acid traffic, a diacylglycerol acyltransferase (DGAT) to catalyze the final step of TAG biosynthesis, and a TAG storage mode. We examined the expression of genes encoding five members of the FABP family and two DGAT proteins, as well as the lipid droplet protein perilipin 2 (PLIN2), during mouse testis development and in specific cells from seminiferous epithelium. Fabp5 expression was distinctive of Sertoli cells and consequently was higher in prepubertal than in adult testis. The expression of Fabp3 increased in testis during postnatal development, associated with the functional differentiation of interstitial cells, but was low in germ cells. Fabp9, together with Fabp12, was prominently expressed in the latter. Their transcripts increased from spermatocytes to spermatids and, interestingly, were highest in spermatid-derived residual bodies (RB). Both Sertoli and germ cells, which produce neutral lipids and store them in lipid droplets, expressed Plin2. Yet, while Dgat1 was detected in Sertoli cells, Dgat2 accumulated in germ cells with a similar pattern of expression as Fabp9. These results correlated with polyunsaturated fatty acid-rich TAG levels also increasing with mouse germ cell differentiation highest in RB, connecting DGAT2 with the biosynthesis of such TAGs. The age-and germ cell type-associated increases in Fabp9, Dgat2, and Plin2 levels are thus functionally related in the last stages of germ cell differentiation.

show abstract

“…However, the mechanisms by which DHA can directly affect the invasive phenotype of cancer cells remain unclear. AA has promigratory and tumour growth properties 96 , which can be antagonized by DHA 97,98 . It has been suggested that the relative levels of DHA and AA in the tumour environment might have a profound impact on tumour growth properties.…”

Section: Molecular Mechanisms Of Actionmentioning

confidence: 99%

The effects of PEDF on cancer biology: mechanisms of action and therapeutic potential

2013

View full text Add to dashboard Cite

The potent actions of pigment epithelium-derived factor (PEDF) on tumour-associated cells, and its extracellular localization and secretion, stimulated research on this multifunctional serpin. Such studies have identified several PEDF receptors and downstream signalling pathways. Known cellular PEDF responses have expanded from the initial discovery that PEDF induces retinoblastoma cell differentiation to its anti-angiogenic, antitumorigenic and antimetastatic properties. Although the diversity of PEDF activities seems to be complex, they are consistent with the varied mechanisms that regulate this multimodal factor. If PEDF is to be used for cancer management, a deeper appreciation of its many functions and mechanisms of action is needed.

show abstract

Brain Fatty Acid-binding Protein and ω-3/ω-6 Fatty Acids

Cited by 93 publications

References 56 publications

CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid

CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid

Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases, and perilipin 2

The effects of PEDF on cancer biology: mechanisms of action and therapeutic potential

Contact Info

Product

Resources

About