Brain distribution pharmacokinetics and integrated pharmacokinetics of Panax Notoginsenoside R1, Ginsenosides Rg1, Rb1, Re and Rd in rats after intranasal administration of Panax Notoginseng Saponins assessed by UPLC/MS/MS

Guo, Qing-li; Li, Pengyue; Wang, Zhen; Cheng, Ye; Wu, Huichao; Yang, Bing; Du, Shouying; Lu, Yang

doi:10.1016/j.jchromb.2014.08.034

Cited by 45 publications

(26 citation statements)

References 18 publications

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“…Owing to the significant pharmacological activities between the two groups of the active components from SM and PN, it is necessary to develop a more efficient method for simultaneous detection of phenolic acids and triterpene saponins which are as the main active biomarkers in Dantonic pill. However, until now, only in vitro and in vivo quantitative methods for detecting either phenolic acids or triterpene saponins have been reported exhaustively (Deng, Wang, Meng, Hu, & Yao, ; Guo et al, ; Li et al, , ; Liu, Li, Li, Wang, & Xue, ; Liu et al, ; Song, Zhang, Xu, Hang, & Jia, ; Wu, Liu, Bai, Lu, & Du, ; Wu, Yee, Jiang, & Cai, ; Zhao et al ., ). So far several methods have been reported for analysis of multiple constituents in rat following oral administration of Compound Danshen preparation (Lv et al, ; Wei et al, ), while they focused on characterization analysis of origin chemicals and metabolites.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by LC–MS/MS and its application

Yang

et al. 2017

Biomedical Chromatography

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Dantonic pill, consisting of Salviae miltiorrhize, Panax notoginseng and Borneol, is a widely used compound Chinese medicine for preventing and treating ischemic cardiovascular diseases in China. In the present study, an original and sensitive method for simultaneous determination of tanshinol (i.e. danshensu), protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by liquid chromatography-tandem mass spectrometry operated in positive/negative ion switching mode was established and validated. The lower limits of quantification for tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 were 5, 0.5, 1, 0.5, 0.5 and 2 ng/mL, respectively. All of the calibration curves showed good linearity over the investigated concentration range (r> 0.99). Validation results demonstrated that the above compounds were accurately, precisely and robustly quantified in rat plasma. The method was successfully applied to characterize the pharmacokinetic profiles of all six compounds in rats following a single oral administration of Dantonic pill.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by LC–MS/MS and its application

Yang

et al. 2017

Biomedical Chromatography

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“…It is well known that multi-active constituents from traditional Chinese medicine are responsible for efficacy and toxicity together, so clarifying the holistic pharmacokinetic profile of traditional Chinese medicine was a tremendous challenge. Therefore, the integrated pharmacokinetics was carried out to describe the overall pharmacokinetics of traditional Chinese medicine to some extent [23]. An area under the curve (AUC)-weighting approach on same type of components from one medicine has been proposed to obtain integrated pharmacokinetic properties, and this approach has been successfully applied and the integrated pharmacokinetic parameters were well correlated with the pharmacodynamic data [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic study of Gastrodia elata in rats

et al. 2015

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The pharmacokinetics of parishin, gastrodin, Gastrodia elata extract and Rhizoma Gastrodiae capsule was investigated by intragastric and/or intravenous administration to rats. Parishin was metabolized into nine metabolites after intravenous administration, and the area under the curve (AUC0-∞) of parishin and its metabolites (except parishin G and parishin E) increased nonlinearly from 72.5 to 220 mg/kg. When combining regression equation with the AUC0-∞ and dose of gastrodin injection, the percent conversion of parishin to gastrodin was obtained as 50 %. Based on multi-active metabolites of parishin in vivo, integrated pharmacokinetic mode was established. It is notable that each metabolite from parishin shares the similar metabolic process at three dosages of parishin and the bioavailability of parishin was approximately 14 %. The integrated pharmacokinetic mode was successfully applied to evaluate the holistic pharmacokinetics of gastrodin injection, G. elata extract and Rhizoma Gastrodiae capsule. The results showed that the holistic pharmacokinetics of gastrodin injection and G. elata extract was closed to that of gastrodin, but for parishin and Rhizoma Gastrodiae capsule, integrated pharmacokinetic parameters were more suitable to evaluate its holistic pharmacokinetics. Graphical abstract Pharmacokinetic study of Gastrodia elata in rats.

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“…Rc has exhibited effects on protection of central nervous system [7,8], prevention of diabetes [9], anti-in ammatory [10], antioxidation [11] and anti-adipogenesis [12]. In addition, Rc can be absorbed into blood and brain tissue rapidly after administration [13]. Researches have shown that ginsenosides are effective in treating cerebral I/R and other nervous system diseases [14].…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rc Protects Neurons From Oxygen-glucose Deprivation Injuries and Its Mechanistic Investigation via a Network Pharmacology-based Analysis

Huang

Chen

Zheng

et al. 2020

Preprint

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Background: Ginsenoside Rc (Rc) is one of the major active components of Panax ginseng Meyer. Studies have shown that Rc has remarkable effect in protection of nervous system. However, the potential molecular mechanism of its neuroprotective effect remains unclear. Our study aim to investigate the neuroprotective effect of Rc on neuron damage and explore the potential mechanism on its regulation of TNF-α and DRP-1.Methods: Oxygen-glucose deprivation reperfusion (OGD/R) cell neuron damage modle was induced by Na2S2O4 and EBSS solution. After preventive administration, cell viability and cell toxicity were detected to evaluate the putative neuroprotective properties of Rc. Network pharmacology and molecular docking simulation studies were performed to predict the potential targets and pharmacological mechanism. Furthermore, the prediction was validated via western blot assay and specific antagonist. Results: In OGD/R injured cells, Rc significantly improved cell viability (Rc middle dose vs. OGD/R model: 67.3±2.33% vs. 55.7±1.14%, P<0.05) and obviously decreased cell toxicity (Rc middle dose vs. OGD/R model: 147±39.7% vs. 232±29.4%, P<0.01). Analysis of network pharmacology and molecular docking indicated that the key targets of Rc are TNF-α and DRP-1. Subsequently molecular biological studies showed a significant increase on expression of TNF-α and DRP-1 in model group. Conversely, administration of Rc reversed the alteration significantly and presented a dose dependence. By adding antagonist, we validated that Rc had an indirect regulation on TNF-α and DRP-1. Conclusions: Rc possess protective properties against OGD-induced neuron damage by regulating the expression of TNF-α and DRP-1.

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Brain distribution pharmacokinetics and integrated pharmacokinetics of Panax Notoginsenoside R1, Ginsenosides Rg1, Rb1, Re and Rd in rats after intranasal administration of Panax Notoginseng Saponins assessed by UPLC/MS/MS

Cited by 45 publications

References 18 publications

Simultaneous determination of tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by LC–MS/MS and its application

Simultaneous determination of tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by LC–MS/MS and its application

Pharmacokinetic study of Gastrodia elata in rats

Ginsenoside Rc Protects Neurons From Oxygen-glucose Deprivation Injuries and Its Mechanistic Investigation via a Network Pharmacology-based Analysis

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