Brain-derived Neurotrophic Factor Regulates Satellite Cell Differentiation and Skeltal Muscle Regeneration

Clow, Charlene; Jasmin, Bernard J.

doi:10.1091/mbc.e10-02-0154

Cited by 133 publications

(130 citation statements)

References 44 publications

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“…RNA samples were quantified using the Gene Quant II spectrophotometer (GE Healthcare). Reverse transcription (RT) reactions were performed as previously described (Clow and Jasmin, 2010).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of Multiple Exon 1 Variants in Mammalian HuD mRNA and Neuron-Specific Transcriptional Control via Neurogenin 2

2012

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The RBP (RNA-binding protein) and Hu/ELAV family member HuD regulates mRNA metabolism of genes directly or indirectly involved in neuronal differentiation, learning and memory, and several neurological diseases. Given the important functions of HuD in a variety of processes, we set out to determine the mechanisms that promote HuD mRNA expression in neurons using a mouse model. Through several complementary approaches, we determined that the abundance of HuD mRNA is predominantly under transcriptional control in developing neurons. Bioinformatic and 5ЈRACE (rapid amplification of cDNA ends) analyses of the 5Ј genomic flanking region identified eight conserved HuD leader exons (E1s), two of which are novel. Expression of all E1 variants was determined in mouse embryonic (E14.5) and adult brains. Sequential deletion of the 5Ј regulatory region upstream of the predominantly expressed E1c variant revealed a well conserved 400 bp DNA region that contains five E-boxes and is capable of directing HuD expression specifically in neurons. Using EMSA (electrophoretic mobility shift assay), ChIP (chromatin immunoprecipitation), and 5Ј regulatory region deletion and mutation analysis, we found that two of these E-boxes are targets of Neurogenin 2 (Ngn2) and that this mechanism is important for HuD mRNA induction. Together, our findings reveal that transcriptional regulation of HuD involves the use of alternate leader exons and Ngn2 mediates neuron-specific mRNA expression. To our knowledge, this is the first study to identify molecular events that positively regulate HuD mRNA expression.

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“…RNA samples were quantified using the Gene Quant II spectrophotometer (GE Healthcare). Reverse transcription (RT) reactions were performed as previously described (Clow and Jasmin, 2010).…”

Section: Methodsmentioning

confidence: 99%

“…Annealing temperature and PCR cycle numbers within linear range of amplification were first determined for each primer set (Table 1). Real-time quantitative PCR was performed as previously described (Clow and Jasmin, 2010). Annealing temperatures for all primer sets were 60°C.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Multiple Exon 1 Variants in Mammalian HuD mRNA and Neuron-Specific Transcriptional Control via Neurogenin 2

2012

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“…In cultured primary myoblasts, the expression of BDNF is decreased dramatically during myogenic differentiation; in concordance, a reduction in endogenous BDNF leads to early myogenic differentiation, while addition of exogenous BDNF can reverse this phenotype (358). In vivo, BDNF Ϫ/Ϫ satellite cells are decreased and hence compromise muscle regeneration (102). These observations suggest that the function of BDNF in adult skeletal muscle is to maintain satellite cells in their quiescent state and prevent their myogenic differentiation (358).…”

Section: Local Milieumentioning

confidence: 98%

Satellite Cells and the Muscle Stem Cell Niche

Yin

Price

Rudnicki

2013

Physiological Reviews

1,662

1,808

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LYin H, Price F, Rudnicki MA. Satellite Cells and the Muscle Stem Cell Niche. Physiol Rev 93: 23-67, 2013; doi:10.1152/physrev.00043.2011.-Adult skeletal muscle in mammals is a stable tissue under normal circumstances but has remarkable ability to repair after injury. Skeletal muscle regeneration is a highly orchestrated process involving the activation of various cellular and molecular responses. As skeletal muscle stem cells, satellite cells play an indispensible role in this process. The self-renewing proliferation of satellite cells not only maintains the stem cell population but also provides numerous myogenic cells, which proliferate, differentiate, fuse, and lead to new myofiber formation and reconstitution of a functional contractile apparatus. The complex behavior of satellite cells during skeletal muscle regeneration is tightly regulated through the dynamic interplay between intrinsic factors within satellite cells and extrinsic factors constituting the muscle stem cell niche/microenvironment. For the last half century, the advance of molecular biology, cell biology, and genetics has greatly improved our understanding of skeletal muscle biology. Here, we review some recent advances, with focuses on functions of satellite cells and their niche during the process of skeletal muscle regeneration.

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“…To date, it has been generally known that neurotrophins play a role in regulating several factors including neuronal survival, plasticity, growth, and death. However, accumulating evidence suggests that neurotrophins influence the development, function, and regeneration of skeletal muscle [17]. It is suggested that NGF is involved in muscle regeneration among various neurotrophins in particular.…”

Section: Resultsmentioning

confidence: 99%

Pulsed Electromagnetic Fields to Influence Pain and Muscle Healing Following Muscle Injury in Rats

Koo¹,

Na²,

Yong³

2015

Journal of Magnetics

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Muscle contusion has a negative effect on muscle function. Although several studies showed that pain control and muscle recovery is facilitated by pulsed electromagnetic fields (PEMF), there has not been much research regarding the specific effects of PEMF on them. The aim of the present study is to investigate effects of PEMF on pain and muscle recovery following extensor digitorum longus (EDL) contusion injury through measuring the expression of the c-fos proto-oncogene and nerve growth factor (NGF). Significantly reduced c-fos expression in the spinal cord was shown in PEMF groups compared with control (CON) groups. There was no significant difference between PEMF1 and CON1, but significantly increased NGF expression was shown in PEMF3 and PEMF5 compared with in CON groups, where the numbers in the group names are the days from contusion. In conclusion, PEMF could be used to not only reduce pain in muscle injuries by down-regulating cfos expression in the spinal cord, but it could also influence muscle healing through increasing NGF expression in the injured muscle.

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Brain-derived Neurotrophic Factor Regulates Satellite Cell Differentiation and Skeltal Muscle Regeneration

Abstract: In this study, muscle-specific BDNF knockout animals were generated and compared with BDNF−/− knockouts. Our findings show that muscle-derived BDNF plays an important role in 1) regulating satellite cell proliferation and differentiation and 2) early regeneration after muscle injury.

Cited by 133 publications

References 44 publications

Characterization of Multiple Exon 1 Variants in Mammalian HuD mRNA and Neuron-Specific Transcriptional Control via Neurogenin 2

Characterization of Multiple Exon 1 Variants in Mammalian HuD mRNA and Neuron-Specific Transcriptional Control via Neurogenin 2

Satellite Cells and the Muscle Stem Cell Niche

Pulsed Electromagnetic Fields to Influence Pain and Muscle Healing Following Muscle Injury in Rats

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