Brain derived neurotrophic factor mediates accelerated recovery of regenerative electrical stimulation in an animal model of stress urinary incontinence

Balog, Brian; Deng, Kangli; Askew, Tessa; Kuang, Mei; Hanzlicek, Brett; Damaser, Margot S.

doi:10.1016/j.expneurol.2021.113781

Cited by 6 publications

(5 citation statements)

References 31 publications

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“…Furthermore, BDNF has been shown to be important to PN recovery since inhibiting the BDNF regenerative pathway delays functional recovery of the PN motor branch, suggesting that the decreased BDNF could result in impaired PN motor branch regeneration (Balog et al, 2020). Electrical stimulation of the PN has been shown to accelerate recovery of the continence mechanism via a BDNF-mediated mechanism (Jiang et al, 2018;Balog et al, 2021). The results from this study with that of the studies mentioned above, support the hypothesis that upregulation of BDNF is key for recovery from a combinatorial neuromuscular injury, in childbirth and in other neuromuscular injures.…”

Section: Discussionmentioning

confidence: 99%

“…In vivo functional tests, including LPP, EUS EMG, and PNSBP, were performed as described previously (Deng et al, 2019;Balog et al, 2021). Under 2% isoflurane anesthesia, a PE-50 polyethylene catheter was inserted into the rat bladder dome and a purse-string suture was used to fix the catheter before closing the abdominal wall.…”

Section: Functional Testing: Urinary Function and Pudendal Nerve Sens...mentioning

confidence: 99%

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Restoring Functional Behaviors After Traumatic Peripheral Nerve Injuries

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Section: Discussionmentioning

confidence: 99%

Section: Functional Testing: Urinary Function and Pudendal Nerve Sens...mentioning

confidence: 99%

Restoring Functional Behaviors After Traumatic Peripheral Nerve Injuries

2024

Frontiers Research Topics

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“…Furthermore, BDNF has been shown to be important to PN recovery since inhibiting the BDNF regenerative pathway delays functional recovery of the PN motor branch, suggesting that the decreased BDNF could result in impaired PN motor branch regeneration (Balog et al, 2020 ). Electrical stimulation of the PN has been shown to accelerate recovery of the continence mechanism via a BDNF-mediated mechanism (Jiang et al, 2018 ; Balog et al, 2021 ). The results from this study with that of the studies mentioned above, support the hypothesis that upregulation of BDNF is key for recovery from a combinatorial neuromuscular injury, in childbirth and in other neuromuscular injures.…”

Section: Discussionmentioning

confidence: 99%

“…In vivo functional tests, including LPP, EUS EMG, and PNSBP, were performed as described previously (Deng et al, 2019 ; Balog et al, 2021 ). Under 2% isoflurane anesthesia, a PE-50 polyethylene catheter was inserted into the rat bladder dome and a purse-string suture was used to fix the catheter before closing the abdominal wall.…”

Section: Methodsmentioning

confidence: 99%

Brain-Derived Neurotrophic Factor Is an Important Therapeutic Factor in Mesenchymal Stem Cell Secretions for Treatment of Traumatic Peripheral Pelvic Injuries

Yuan

Balog

Lin

et al. 2022

Front. Cell. Neurosci.

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Traumatic neuromuscular injury to the pudendal nerve and urethra during childbirth does not regenerate well and contributes to stress urinary incontinence in women. Mesenchymal stem cells (MSCs) can improve neuroregeneration via their secretions, or secretome, which includes brain-derived neurotrophic factor (BDNF). In this study, we investigated whether BDNF is a key factor in the secretome of MSCs for the facilitation of functional recovery following a dual simulated childbirth injury. BDNF knockdown (KD) MSCs were created using an anti-BDNF shRNA lentivirus vector. A scrambled sequence was used as a transduction control (scrambled). Cells were cultured for 24 h before media was concentrated 50x to create concentrated conditioned media (CCM) containing MSC secretome. CCM of unmanipulated MSCs was screened for high BDNF expression (high BDNF CCM). Concentrated control media (CM) was created by concentrating media not conditioned by cells. Female Sprague-Dawley rats underwent bilateral pudendal nerve crush and vaginal distension (Injury) or sham injury. One hour and 1 week after injury, sham injured rats received CM, and injured rats received CM, high BDNF CCM, KD CCM, or scrambled CCM (300 μl intraperitoneally). Three weeks after injury, rats underwent leak point pressure (LPP) and pudendal nerve sensory branch potential (PNSBP) recordings. The urethra and pudendal nerve were harvested for anatomical assessment. ANOVA followed by the Student-Newman-Keuls test determined significant differences between groups (p < 0.05). BDNF KD CCM had significantly decreased BDNF concentration compared to scrambled CCM, while the concentration in high BDNF CCM was significantly increased. LPP was significantly decreased in CM and KD CCM treated animals compared to sham injury, but not with scrambled or high BDNF CCM. PNSBP firing rate showed a significant decrease with CM treatment compared to sham injury. Neuromuscular junctions in the urethral sphincter in KD CCM, scrambled CCM, and high BDNF CCM were healthier than CM treated rats. While anatomical and nerve function tests demonstrate regeneration of the pudendal nerve with any CCM treatment, LPP results suggest it takes longer to recover continence with reduced BDNF in CCM. BDNF in MSC CCM is an important factor for the acceleration of recovery from a dual nerve and muscle injury.

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“…It is evident that postpartum depression and postpartum SUI share some pathological factors. Several clinical trials have shown a link between brain-derived neurotrophic factor (BDNF) and SUI [54]; and observations in animal models suggest that increased levels of BDNF in Serum contribute to the resurrection of the external nerves of the urethral sphincter, and that BDNF is essential for normal sphincter function [55,56]. BDNF decreases significantly during pregnancy and even keep low levels in the 2-3 months after delivery [57].…”

Section: Changes In Central and Peripheral Neurotransmittersmentioning

confidence: 99%

Research Progress of Postpartum Stress Urinary Incontinence

Jia

2023

TNS

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Postpartum stress urinary incontinence (SUI) is one of the gynecological disorders that plague postpartum women, which severely affects womens daily work and life and may even cause anxiety and depression among women while decreasing their quality of life. In recent years, conservative treatment methods have been explored and applied extensively in postpartum SUI rehabilitation. Experimental data suggest that providing specific treatments for specific groups can greatly improve the efficiency of treatment and that classifying different types of treatments can also effectively promote the advancement of related diagnostic methods. This article systematically reviews the literature on the pathological mechanisms, and conservative treatment of postpartum urinary incontinence (UI), and discusses the optimal treatment methods for different etiologies, providing some insights for the rehabilitation and management of patients with postpartum SUI.

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Brain derived neurotrophic factor mediates accelerated recovery of regenerative electrical stimulation in an animal model of stress urinary incontinence

Cited by 6 publications

References 31 publications

Restoring Functional Behaviors After Traumatic Peripheral Nerve Injuries

Restoring Functional Behaviors After Traumatic Peripheral Nerve Injuries

Brain-Derived Neurotrophic Factor Is an Important Therapeutic Factor in Mesenchymal Stem Cell Secretions for Treatment of Traumatic Peripheral Pelvic Injuries

Research Progress of Postpartum Stress Urinary Incontinence

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