Brain derived neurotrophic factor expression and DNA methylation in response to subchronic valproic acid and/or aldosterone treatment

Buzgoova, K.; Graban, Jan; Balagova, Lucia; Hlavacova, Natasa; Ježová, Daniela

doi:10.3325/cmj.2019.60.71

Cited by 9 publications

(7 citation statements)

References 33 publications

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“…In an animal model of SCI and under conditions of cellular excito- toxicity, VPA functions via HDAC inhibition, which increases cell viability, enhances BDNF expression, prevents the activation of NF-kB, and inhibits ER stress (Scheuing et al, 2014). However, there are some studies describing an actual decrease in the gene expression levels of BDNF in the brain after VPA administration (Umka et al, 2010;Buzgoova et al, 2019). It has been well-documented that VPA performs an important role in the treatment of CNS damage, via the induction of neuronal differentiation and the enhancement of neural growth (Kim et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Investigating the synergic effects of valproic acid and crocin on BDNF and GDNF expression in epidermal neural crest stem cells

Baharvand¹,

Nabiuni²,

Tahmaseb³

et al. 2020

Acta Neurobiologiae Experimentalis

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Following nerve tissue damage, various events, such as inflammatory responses, microglial activation, endoplasmic reticulum stress, and apoptosis, can occur, which all lead to cell death, prevent axonal growth, and cause axonal circumvolution. So far, several researchers have tended to adopt strategies to reduce the harmful conditions associated with neurological disorders, and stem-cell-based therapy is one of those promising strategies. Epidermal neural crest stem cells (EPI-NCSCs) are a type of stem cell that has widely been employed for the treatment of various neurological disorders. It has been suggested that these stem cells perform their supportive actions primarily through the release of different neurotrophic factors. Hence, in this study, the neuroprotective impacts of valproic acid (VPA) and crocin were evaluated on the mRNA expression levels of brain-derived neurotrophic factor (BDNF) and glial-cell-derived neurotrophic factor (GDNF) in EPI-NCSCs. In this research, we isolated EPI-NCSCs from the hair follicles of the rat whisker pad. Then, the cells were treated with different concentrations of VPA and crocin for 72 h.Subsequently, an MTT assay was performed to define the suitable concentrations of drugs. Finally, real-time PCR was performed to evaluate the mRNA expression levels of BDNF and GDNF in these stem cells. The results of the MTT assay showed that the treatment of EPI-NCSCs with 1 mM VPA and 1.5 mM crocin, and the co-treatment with 1 mM VPA and 500 µM crocin, led to the survival and proliferation of these stem cells. Moreover, the real-time PCR results revealed that both VPA and crocin, both individually and in combination, can significantly increase the expression levels of BDNF and GDNF in EPI-NCSCs. According to the findings of this study, both VPA and crocin, alone and in combination, are potential candidates for enhancing the capacity of EPI-NCSCs to differentiate into neural lineages. Therefore, the co-treatment of EPI-NCSCs with these drugs can be employed for the treatment of various neurological disorders, such as spinal cord injury.

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Section: Discussionmentioning

confidence: 99%

Investigating the synergic effects of valproic acid and crocin on BDNF and GDNF expression in epidermal neural crest stem cells

Baharvand¹,

Nabiuni²,

Tahmaseb³

et al. 2020

Acta Neurobiologiae Experimentalis

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“…In conclusion, the present study provides evidence on antidepressant effects of valproic acid using a classical behavioral approach for testing the efficacy of antidepressant drug in animal models. It may be related to the epigenetic modulations induced by the same dose of valproic acid described recently (Buzgoova et al 2019).…”

Section: Discussionmentioning

confidence: 73%

Antidepressant effects of valproic acid in an animal model of depression

Buzgoova

Balagova

Hlavacova

et al. 2019

European Pharmaceutical Journal

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Valproic acid, beside its anticonvulsant action, is widely used as a mood stabilizer in the therapy of bipolar disorder. The potential antidepressant action of valproic acid has not been sufficiently characterized so far. The aim of the present study was to evaluate the antidepressant effect of valproic acid in an aldosterone model of depression. Subchronic treatment with valproic acid resulted in a reduction of the time spent in immobility in the forced swim test. In conclusion, the present study provides evidence on antidepressant effects of valproic acid using a classical behavioral approach for testing the efficacy of antidepressant drug in animal models.

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“…In the case of small intestine, its content was removed before tissue homogenisation. Total RNA extraction, transcription of mRNA into cDNA as well as gene expression quantification was performed as described previously (Buzgoova et al, 2019). Primer BLAST NCBI software was used to design primers specific for the studied genes as well as reference genes (Tab.…”

Section: Ace2 Mrna and Protein Quantificationmentioning

confidence: 99%

Food Enrichment with Glycyrrhiza Glabra Extract Suppresses ACE2 mRNA and Protein Expression in Rats – Possible Implications for COVID-19

Ježová

Karailiev

Karailievova

et al. 2021

Preprint

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Angiotensin converting enzyme 2 (ACE2) is a key entry point of SARS-CoV-2 virus known to induce COVID-19. We have recently outlined the concept to reduce ACE2 expression by the administration of glycyrrhizin, a component of Glycyrrhiza glabra extract, via its inhibitory activity on 11beta hydroxysteroid dehydrogenase type 2 (11betaHSD2) and resulting activation of mineralocorticoid receptor (MR). We hypothesized that in organs, such as the intestine, which co-express 11betaHSD2, MR and ACE2, the expression of ACE2 would be suppressed. We studied organ tissues from an experiment originally designed to address the effects of Glycyrrhiza glabra extract on the stress response. Male Sprague Dawley rats were left undisturbed or exposed to chronic mild stress for five weeks. For the last two weeks, animals continued with a placebo diet or received a diet containing extract of Glycyrrhiza glabra root at a dose of 150 mg/kg of body weight/day. Quantitative PCR measurements showed a significant decrease in gene expression of ACE2 in the small intestine of rats fed with diet containing Glycyrrhiza glabra extract. This effect was independent of the stress condition and failed to be observed in non-target tissues, namely the heart and the brain cortex. In the small intestine we confirmed the reduction of ACE2 also at the protein level. Present findings provide first evidence to support the hypothesis that Glycyrrhiza glabra extract may reduce an entry point of SARS-CoV-2. Whether this phenomenon, when confirmed in additional studies, is linked to the susceptibility of cells to the virus requires further studies.

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Brain derived neurotrophic factor expression and DNA methylation in response to subchronic valproic acid and/or aldosterone treatment

Cited by 9 publications

References 33 publications

Investigating the synergic effects of valproic acid and crocin on BDNF and GDNF expression in epidermal neural crest stem cells

Investigating the synergic effects of valproic acid and crocin on BDNF and GDNF expression in epidermal neural crest stem cells

Antidepressant effects of valproic acid in an animal model of depression

Food Enrichment with Glycyrrhiza Glabra Extract Suppresses ACE2 mRNA and Protein Expression in Rats – Possible Implications for COVID-19

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