Brain-derived neurotrophic factor (BDNF) is expressed in buffalo (Bubalus bubalis) ovarian follicles and promotes oocyte maturation and early embryonic development

Zhao, Xin; Du, Fuyou; Liu, Xiaolin; Ruan, Qiurong; Wu, Zhulian; Lei, Chuan; Deng, Yanfei; Luo, Chen; Jiang, Jianrong; Shi, Dongquan; Lu, Fenghua

doi:10.1016/j.theriogenology.2019.02.020

Cited by 34 publications

(25 citation statements)

References 42 publications

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“…IVF was based on procedures reported in one previous study (Zhao et al, 2019 a , 2019 b ). Oocytes at the MII stage were washed twice in modified Tyrode's medium, about 15 oocytes were transferred into droplets made by 25 μ L modified Tyrode's medium covered with mineral oil.…”

Section: Methodsmentioning

confidence: 99%

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Histone Demethylase KDM4D Could Improve the Developmental Competence of Buffalo (Bubalus Bubalis) Somatic Cell Nuclear Transfer (SCNT) Embryos

Feng

Zhao

et al. 2021

Microsc Microanal

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Somatic cell nuclear transfer (SCNT) holds vast potential in agriculture. However, its applications are still limited by its low efficiency. Histone 3 lysine 9 trimethylation (H3K9me3) was identified as an epigenetic barrier for this. Histone demethylase KDM4D could regulate the level of H3K9me3. However, its effects on buffalo SCNT embryos are still unclear. Thus, we performed this study to explore the effects and underlying mechanism of KDM4D on buffalo SCNT embryos. The results revealed that compared with the IVF embryos, the expression level of KDM4D in SCNT embryos was significantly lower at 8- and 16-cell stage, while the level of H3K9me3 in SCNT embryos was significantly higher at 2-cell, 8-cell, and blastocyst stage. Microinjection of KDM4D mRNA could promote the developmental ability of buffalo SCNT embryos. Furthermore, the expression level of ZGA-related genes such as ZSCAN5B, SNAI1, eIF-3a, and TRC at the 8-cell stage was significantly increased. Meanwhile, the pluripotency-related genes like POU5F1, SOX2, and NANOG were also significantly promoted at the blastocyst stage. The results were reversed after KDM4D was inhibited. Altogether, these results revealed that KDM4D could correct the H3K9me3 level, increase the expression level of ZGA and pluripotency-related genes, and finally, promote the developmental competence of buffalo SCNT embryos.

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Section: Methodsmentioning

confidence: 99%

“…In vitro culture (IVC) was based on procedures reported in one previous study (Zhao et al, 2019 a , 2019 b ). The SCNT embryos and IVF embryos were co-cultured with granulosa cell monolayer in a droplet of CM (15 embryos/25 μ L) overlaid with mineral oil at 38.5°C under a humidified atmosphere of 5% CO 2 in air at 38.5°C.…”

Section: Methodsmentioning

confidence: 99%

Histone Demethylase KDM4D Could Improve the Developmental Competence of Buffalo (Bubalus Bubalis) Somatic Cell Nuclear Transfer (SCNT) Embryos

Feng

Zhao

et al. 2021

Microsc Microanal

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“…Recent studies have shown that natriuretic peptide type C (CNP) secreted by GCs improved developmental capacity of oocytes from mice and sheep (Rouhollahi Varnosfaderani, Hajian, Jafarpour, Ghazvini Zadegan, & Nasr‐Esfahani, 2020; Zhang, Su, Sugiura, Xia, & Eppig, 2010). Brain‐derived neurotrophic factor (BDNF) also secreted by GCs promoted maturation rate and subsequent embryonic formation of buffalo oocytes (Zhao et al., 2019). Insulin‐like growth factor‐I (IGF‐I) synthesized by GCs also plays an important role in maturation and subsequent developmental competence of porcine oocytes (Oberlender et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

Theca cell‐conditioned medium added to in vitro maturation enhances embryo developmental competence of buffalo (Bubalus bubalis) oocytes after parthenogenic activation

Zhang

Deng

Chen

et al. 2020

Reprod Domestic Animals

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In vitro maturation (IVM) of mammalian oocytes has been a basic and key technology in embryo engineering, which also includes somatic cell nuclear transfer and the production of transgenic animals. An important aspect of embryo engineering technology is acquiring high-quality oocytes. Although IVM technology is established and has seen great progress, the efficiency of IVM technology is still lower than

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“…Oocytes in follicles are tightly surrounded by cumulus cells and cannot directly obtain signals and nutrition from the microenvironment of the ovary. Therefore, cellular interaction between oocytes and somatic cells plays an indispensable role in oocyte growth and maturation and folliculogenesis [24][25][26]. One study showed that mice with disrupted GJs failed to undergo oocyte meiotic maturation and were infertile [17].…”

Section: Discussionmentioning

confidence: 99%

Involvement of GJA1 and Gap Junctional Intercellular Communication between Cumulus Cells and Oocytes from Women with PCOS

Liu

Kong

Zhang

et al. 2020

BioMed Research International

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Polycystic ovary syndrome (PCOS) is a common female endocrine system disease that affects 17.8% of women of reproductive age and leads to infertility, obesity, glucose metabolic disorders, cardiovascular disease, and body-mind problems. However, the etiology of PCOS remains unclear. Follicular growth is disrupted as a result of ovarian hyperandrogenism and distorted intraovarian paracrine signaling in women with PCOS. Microcommunication between oocytes and cumulus cells plays a critical role in folliculogenesis. Gap junction alpha 1 (GJA1) plays a crucial role in the developing follicles by forming communication channels between cumulus cells and oocytes, but this has not yet been reported in women with PCOS. Therefore, we aimed to study the role of GJA1 in the microcommunication between oocytes and cumulus cells in women with PCOS. In our study, cumulus cell-oocyte complexes (COCs) from women were isolated via ultrasound-guided vaginal puncture, and oocytes were selected from COCs and categorized based on 3 oocyte maturation stages. Then, RT-qPCR and immunofluorescence analysis were performed to detect both the gene expression and protein of GJA1 in oocytes from women with and without PCOS. There was no statistically significant difference in age and BMI (body mass index), but patients with PCOS had a higher ratio of basic LH/FSH (luteinizing hormone/follicle-stimulating hormone), androstenedione, and total ovarian volume. The qRT-PCR results showed higher gene expression of GJA1 in oocytes without PCOS at the germinal vesicle (GV) stage compared with that of oocytes from women with PCOS. Immunofluorescence analysis showed that the expression level of GJA1 in oocytes from women with PCOS was very weak compared with that of oocytes from women without PCOS. In conclusion, GJA1 may play a critical role in the development of oogenesis arrest in women with PCOS throughout the oogenesis processes, including oogenesis and oocyte maturation.

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Brain-derived neurotrophic factor (BDNF) is expressed in buffalo (Bubalus bubalis) ovarian follicles and promotes oocyte maturation and early embryonic development

Cited by 34 publications

References 42 publications

Histone Demethylase KDM4D Could Improve the Developmental Competence of Buffalo (Bubalus Bubalis) Somatic Cell Nuclear Transfer (SCNT) Embryos

Histone Demethylase KDM4D Could Improve the Developmental Competence of Buffalo (Bubalus Bubalis) Somatic Cell Nuclear Transfer (SCNT) Embryos

Theca cell‐conditioned medium added to in vitro maturation enhances embryo developmental competence of buffalo (Bubalus bubalis) oocytes after parthenogenic activation

Involvement of GJA1 and Gap Junctional Intercellular Communication between Cumulus Cells and Oocytes from Women with PCOS

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