Brain-derived neurotrophic factor (BDNF) expression in normal and regenerating olfactory epithelium of Xenopus laevis

Frontera, Jimena Laura; Cervino, Ailen S.; Jungblut, Lucas D.; Paz, Dante A.

doi:10.1016/j.aanat.2014.10.010

Cited by 15 publications

(14 citation statements)

References 34 publications

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“…In this regard, BDNF presents a different expression distribution in the OE of X. laevis larvae subjected to ZnSO 4 or axotomy: while BDNF expression increases in some basal cells after ZnSO 4 treatment (Frontera et al, ), no changes in BDNF expression pattern are verified after axotomy (Fig. ).…”

Section: Discussionmentioning

confidence: 97%

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Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis

Cervino

Paz

Frontera

2017

Developmental Neurobiology

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The olfactory epithelium (OE) has the remarkable capability to constantly replace olfactory receptor neurons (ORNs) due to the presence of neural stem cells (NSCs). For this reason, the OE provides an excellent model to study neurogenesis and neuronal differentiation. In the present work, we induced neuronal degeneration in the OE of Xenopus laevis larvae by bilateral axotomy of the olfactory nerves. We found that axotomy induces specific- neuronal death through apoptosis between 24 and 48h post-injury. In concordance, there was a progressive decrease of the mature-ORN marker OMP until it was completely absent 72h post-injury. On the other hand, neurogenesis was evident 48h post-injury by an increase in the number of proliferating basal cells as well as NCAM-180- GAP-43+ immature neurons. Mature ORNs were replenished 21 days post-injury and the olfactory function was partially recovered, indicating that new ORNs were integrated into the olfactory bulb glomeruli. Throughout the regenerative process no changes in the expression pattern of the neurotrophin Brain Derivate Neurotrophic Factor were observed. Taken together, this work provides a sequential analysis of the neurodegenerative and subsequent regenerative processes that take place in the OE following axotomy. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1308-1320, 2017.

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Section: Discussionmentioning

confidence: 97%

“…To asses this, an immunohistochemical analysis was carried out in intact and axotomized larvae. The specificity of BDNF primary antibody has been previously verified (Frontera et al, 2014).…”

Section: Bdnf Expression In the Oe After Axotomymentioning

confidence: 99%

Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis

Cervino

Paz

Frontera

2017

Developmental Neurobiology

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“…One time injection of 50 μg BDNF could successfully improve the morphology and rescue the electrophysiological properties of injured optic nerve in rabbits. BDNF was also shown to enhance regeneration after injury of the cervical spinal cord (Gransee et al 2015), cavernous sinus nerve of the penis (Kim et al 2012), sciatic nerve (Dadon-Nachum et al 2012), optic nerve (Zhang et al 2015), and olfactory epithelium (Frontera et al 2015; Uranagase et al 2012) in animal models.…”

Section: Brain Derived Neurotrophic Factormentioning

confidence: 99%

Neurotrophic Factors and Their Potential Applications in Tissue Regeneration

Xiao¹,

2015

Arch. Immunol. Ther. Exp.

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Neurotrophic factors are growth factors that can nourish neurons and promote neuron survival and regeneration. They have been studied as potential drug candidates for treating neurodegenerative diseases. Since their identification, there are more and more evidences to indicate that neurotrophic factors are also expressed in non-neuronal tissues and regulate the survival, anti-inflammation, proliferation and differentiation in these tissues. This mini review summarizes the characteristics of the neurotrophic factors and their potential clinical applications in the regeneration of neuronal and non-neuronal tissues.

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“…Limb muscle fibers have been reported to express neurotrophins and their receptors not only in the developmental stages, but also during muscle transformation as well as in various physiological and pathological conditions . The presence of neurotrophins in several non‐neuronal cells suggests their wider role apart from the nervous system . The presence of neurotrophins in upper airway muscle fibers has not been investigated previously.…”

Section: Introductionmentioning

confidence: 99%

“…20 The presence of neurotrophins in several non-neuronal cells suggests their wider role apart from the nervous system. 21,22 The presence of neurotrophins in upper airway muscle fibers has not been investigated previously.…”

Section: Introductionmentioning

confidence: 99%

Neurotrophic factor BDNF is upregulated in soft palate muscles of snorers and sleep apnea patients

Shah

Forsgren

Holmlund

et al. 2018

Laryngoscope Investig Oto

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Objectives Neuromuscular injuries are suggested to contribute to upper airway collapse and swallowing dysfunction in patients with sleep apnea. Neurotrophins, a family of proteins involved in survival, development, and function of neurons, are reported to be upregulated in limb muscle fibers in response to overload and nerve damage. We aimed to investigate the expression of two important neurotrophins, brain‐derived neurotrophic factor (BDNF) and nerve growth factor (NGF), in muscle fibers of uvula from snorers and sleep apnea patients and to compare these findings with pharyngeal function. Methods Uvula muscle biopsies from 22 patients and 10 controls were analyzed for BDNF, NGF, and cytoskeletal protein desmin using immunohistochemistry. Pharyngeal swallowing function was assessed using videoradiography. Results BDNF, but not NGF, was significantly upregulated in a subpopulation of muscle fibers in snoring and sleep apnea patients. Two major immunoreaction patterns for BDNF were observed; a fine grainy point like BDNF staining was displayed in muscle fibers of both patients and controls (41 ± 23 vs. 25 ± 17%, respectively, P = .06), while an abnormal upregulated intense‐dotted or disorganized reaction was mainly observed in patients (8 ± 8 vs. 2 ± 2%, P = .02). The latter fibers, which often displayed an abnormal immunoreaction for desmin, were more frequent in patients with than without swallowing dysfunction (10 ± 8 vs. 3 ± 3%, P = .05). Conclusion BDNF is upregulated in the upper airway muscles of snorers and sleep apnea patients, and especially in patients with swallowing dysfunction. Upregulation of BDNF is suggested to be a response to denervation, reinnervation, and repair of injured muscle fibers. Our findings propose that damaged upper airway muscles might heal following treatment for snoring and sleep apnea. Level of Evidence NA

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Brain-derived neurotrophic factor (BDNF) expression in normal and regenerating olfactory epithelium of Xenopus laevis

Cited by 15 publications

References 34 publications

Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis

Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis

Neurotrophic Factors and Their Potential Applications in Tissue Regeneration

Neurotrophic factor BDNF is upregulated in soft palate muscles of snorers and sleep apnea patients

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