Brain and lung cryptococcoma and concurrent corynebacterium pseudotuberculosis infection in a goat: a case report

Luvizotto, Maria Cecília Rui; Carreira, Vinicius; Ferrari, Heitor F.; Ribeiro, Débora da Silva Freitas; Vallim, Marcelo A.; Vasco, Azevedo; Cardoso, Tereza Cristina

doi:10.1590/s1678-91992009000300015

Cited by 6 publications

(12 citation statements)

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“…Nevertheless, the disease has been reported in the dog [18,20,21], cat [18,22], horse [23], cow [19,24], sheep [25,26], and goat [27]. However, most of these cases [20-23, 26, 28] were characterized due to the typical histopathological features of the fungus, while mycological culture [18,25], immunohistochemistry [19,24], and PCR identification [27] were used to confirm the histopathological findings of cryptococcosis.…”

Section: Introductionmentioning

confidence: 99%

“…in domestic animals from Brazil by using molecular techniques was located [18]. Sequencing was not done during the molecular investigation of a goat diagnosed with concomitant cryptococcosis and Johne's disease [27], while fungal sequencing was not possible due to the prolonged fixation of the pathological sample in a case of cerebral cryptococcomas in a cow due to C. neoformans var. grubii [19].…”

Section: Introductionmentioning

confidence: 99%

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Cryptococcus gattii-Induced Infections in Dogs from Southern Brazil

et al. 2015

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Cryptococcus gattii-induced cryptococcosis is an emerging infectious disease of humans and animals worldwide, with rare descriptions of this infection in domestic animals from Brazil. This study presents the findings associated with C. gattii in dogs from Londrina, Paraná, Southern Brazil. Two dogs, a 3-year-old, female German shepherd and a 6-year-old, male Boxer, were evaluated by a combination of pathological, mycological, and molecular diagnostic techniques. Significant pathological alterations included cryptococcal lymphadenitis, meningoencephalitis, tonsillitis, and rhinitis with nasal cryptococcomas in the German shepherd dog, while cryptococcal lymphadenitis and pneumonia were observed in the Boxer; both dogs had pseudocystic cryptococcosis. The mucicarmine histochemical stain readily identified the intralesional cryptococcal budding organisms in all affected tissues. Mycological culture and isolation confirmed the yeasts as C. gattii due to positive reaction with the L-canavanine glycine bromothymol blue agar. A PCR assay using the internal transcribed spacers (ITS)1 and ITS2 primers, which target the ITS1 and 2 regions including the 5.8S rRNA gene, amplified the desired amplicons; direct sequencing confirmed the isolate as C. gattii. ITS nucleotide differentiation demonstrated that the isolate forms part of the ITS type 4 Cryptococcus organisms which corresponds to the C. gattii VGII molecular subtype or the RAPD type 2 Cryptococcus organisms. Collectively, these findings confirmed the participation of C. gattii in the etiopathogenesis of the lesions observed in these dogs and expanded the epidemiological niche of this important mycotic agent to include Southern Brazil. It is noteworthy to mention that previous epidemiological studies have suggested that C. gattii-induced cryptococcosis is more frequently diagnosed in Northern relative to Southern Brazil, so these findings might suggest an expansion of the distribution of this agent within continental Brazil.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryptococcus gattii-Induced Infections in Dogs from Southern Brazil

et al. 2015

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“…The epidemiology of cryptococcosis depends of the fungal species involved; although, it is known that C. neoformans causes disease mainly in immunosuppressed individuals (RODRIGUES et al, 1999). Cryptococcosis in goats is occasionally reported in association with other agents, such as Mycobacterium bovis (GUTIÉRREZ & MARÍN, 1999) and Corynebacterium pseudotuberculosis (LUVIZOTTO et al, 2009). However, consistent lesions with those caused by these agents were not observed in this case.…”

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confidence: 57%

“…In goats, lungs appear to be the main organ affected by the fungus (BARÓ et al, 1998). Although, there are few studies confirming this (GUTIÉRREZ & MARÍN, 1999;LUVIZOTTO et al, 2009). Hence, the aim of this study was to describe the clinical, pathological, and molecular aspects of granulomatous pneumonia by Cryptococcus neoformans in a goat in the Southern region of Brazil.…”

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confidence: 93%

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Pneumonia by Cryptococcus neoformans in a goat in the Southern region of Brazil

et al. 2018

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This report describes the clinical, pathological, and molecular aspects of a pneumonia by Cryptococcus neoformans in a goat in the Southern region of Brazil. A goat with a history of prolonged transportation presented dyspnea, nasal discharge and cough, and was subjected to necropsy, histopathology, and mycological evaluation. Grossly, cranio-ventral pulmonary consolidation was observed, characterized by firm and grayish areas interspersed with dark red foci. Histopathology of the lungs showed areas of parenchymal necrosis, containing blastoconidia with a slightly basophilic central cell, surrounded by an unstained capsule. It was associated with moderate granulomatous inflammatory infiltrate and peripheral fibrosis. The capsule and fungus cell exhibited marked Alcian Blue and periodic acid-Schiff staining, respectively. Diagnosis of fungal pneumonia by C. neoformans was based on clinical, pathological, and molecular findings.

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