BRAF p.Val600Glu (V600E) mutation detection in thyroid fine needle aspiration cell block samples: a feasibility study

Utility of the BRAF p.V600E immunoperoxidase stain in FNA direct smears and cell block preparations from patients with thyroid carcinoma

“…Although the translation of ancillary studies between surgical tissue (formalin‐fixed, paraffin‐embedded) and cytologic preparations may appear insignificant, the preanalytic factors associated with a variety of cytologic substrates (eg, smears vs cell blocks vs liquid‐based cytology, each using different fixatives, preservatives, and stains) require rigorous validation of the testing methodology before implementation in a clinical laboratory that impacts patient management decisions . A study by Leslie et al found that detection of the BRAF mutation in thyroid FNA cell block samples (Bethesda categories of indeterminate and malignant) by combined molecular and IPOX methods demonstrated a sensitivity of 71.4% and a specificity of 100% . In addition, a study by Zimmermann et al demonstrated that IPOX for BRAF p.V600E in PTC cell block sections was highly sensitive and specific (93.8% and 93.8%, respectively) .…”

Predicting Extrathyroidal Extension in Patients With Papillary Thyroid Microcarcinoma According to a BRAF Mutation

“…Therefore, despite the several repetition in our study, our article can be valuable in that we categorize the preoperative feature and showed numerical cutoff value for helping decision making of the surgical extent. Second, a BRAF mutation preoperative analysis using fine needle aspiration cytology can be incorrect [25]. Thus, a false positive or negative BRAF mutation result could lead to an improper application.…”

Diagnostic utility of immunohistochemical analysis and Epstein–Barr virus‐encoded small RNAs in situ hybridisation of cell block sections obtained using fine‐needle aspiration in nasopharyngeal carcinoma with lymph node metastasis

“…1 Over the past few years, several studies have confirmed the diagnostic utility of FNAB in cancer, and therefore, it is now widely used in clinical practice. [2][3][4][5][6][7][8][9][10][11] Owing to innovative developments in FNA technology, we can now collect more cells to prepare cell blocks (CB) that can be used for cell smears and immunohistochemistry (IHC) staining analysis, which compensates for some of the shortcomings of simple smears in organisational structure and cell morphology. IHC analysis represents an important technique to improve the accuracy of FNAB diagnoses.…”

“…IHC analysis represents an important technique to improve the accuracy of FNAB diagnoses. 4,5,8,12,13 In this study, we aimed to explore the feasibility of the combination of IHC analysis and Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) in situ hybridisation of CB sections prepared via FNA for diagnosing metastatic lymph nodes from nasopharyngeal carcinoma (NPC) and comparing this method with simple cell smears and cell smears combined with CBs prepared via FNAB.…”

“…Owing to innovative developments in FNA technology, we can now collect more cells to prepare cell blocks (CB) that can be used for cell smears and immunohistochemistry (IHC) staining analysis, which compensates for some of the shortcomings of simple smears in organisational structure and cell morphology. IHC analysis represents an important technique to improve the accuracy of FNAB diagnoses . In this study, we aimed to explore the feasibility of the combination of IHC analysis and Epstein–Barr virus (EBV)‐encoded small RNAs (EBERs) in situ hybridisation of CB sections prepared via FNA for diagnosing metastatic lymph nodes from nasopharyngeal carcinoma (NPC) and comparing this method with simple cell smears and cell smears combined with CBs prepared via FNAB.…”