Bradyrhizobium arachidis sp. nov., isolated from effective nodules of Arachis hypogaea grown in China

Wang, Rui; Chang, Yue; Zheng, Wen; Zhang, Dan; Zhang, Xiao Xia; Sui, Xin; Wang, En Tao; Hu, Jia Qi; Zhang, Li Ya; Chen, Wen Xin

doi:10.1016/j.syapm.2012.10.009

Cited by 84 publications

(45 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S2 in the supplemental material) means that the symbiotic genes were maintained mainly by vertical transmission and rarely by lateral transfer in the E. fordii-associated rhizobia, in agreement with previous reports (5,46,47). The fact that rhizobia isolated from other legumes grown in the same region as E. fordii, such as peanut plants (48) and soybean (47), harbored diverse symbiotic genes differing from those of the E. fordii rhizobia also supports the above estimations.…”

Section: Discussionsupporting

confidence: 90%

Genetic Diversity and Evolution of Bradyrhizobium Populations Nodulating Erythrophleum fordii, an Evergreen Tree Indigenous to the Southern Subtropical Region of China

Yao

Wang

Lü

et al. 2014

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

The nodulation of Erythrophleum fordii has been recorded recently, but its microsymbionts have never been studied. To investigate the diversity and biogeography of rhizobia associated with this leguminous evergreen tree, root nodules were collected from the southern subtropical region of China. A total of 166 bacterial isolates were obtained from the nodules and characterized. In a PCR-based restriction fragment length polymorphism (RFLP) analysis of ribosomal intergenic sequences, the isolates were classified into 22 types within the genus Bradyrhizobium. Sequence analysis of 16S rRNA, ribosomal intergenic spacer (IGS), and the housekeeping genes recA and glnII classified the isolates into four groups: the Bradyrhizobium elkanii and Bradyrhizobium pachyrhizi groups, comprising the dominant symbionts, Bradyrhizobium yuanmingense, and an unclassified group comprising the minor symbionts. The nodC and nifH phylogenetic trees defined five or six lineages among the isolates, which was largely consistent with the definition of genomic species. The phylogenetic results and evolutionary analysis demonstrated that mutation and vertical transmission of genes were the principal processes for the divergent evolution of Bradyrhizobium species associated with E. fordii, while lateral transfer and recombination of housekeeping and symbiotic genes were rare. The distribution of the dominant rhizobial populations was affected by soil pH and effective phosphorus. This is the first report to characterize E. fordii rhizobia.

show abstract

Section: Discussionsupporting

confidence: 90%

Genetic Diversity and Evolution of Bradyrhizobium Populations Nodulating Erythrophleum fordii, an Evergreen Tree Indigenous to the Southern Subtropical Region of China

Yao

Wang

Lü

et al. 2014

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Consistent with a previous report (Wang et al , 2013), two major groups of species of the genus Bradyrhizobium were evident in phylogenetic trees of 16S rRNA gene sequences: one represented by Bradyrhizobium japonicum and the other by Bradyrhizobium elkanii (Fig. S2).…”

supporting

confidence: 91%

Bradyrhizobium ottawaense sp. nov., a symbiotic nitrogen fixing bacterium from root nodules of soybeans in Canada

Cloutier

Tambong

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Sixteen strains of symbiotic bacteria from root nodules of Glycine max grown in Ottawa, Canada, were previously characterized and placed in a novel group within the genus Bradyrhizobium. To verify their taxonomic status, these strains were further characterized using a polyphasic approach. All strains possessed identical 16S rRNA gene sequences that were 99.79 % similar to the closest relative, Bradyrhizobium liaoningense LMG 18230T. Phylogenetic analysis of concatenated atpD, glnII, recA, gyrB, rpoB and dnaK genes divided the 16 strains into three multilocus sequence types that were placed in a highly supported lineage distinct from named species of the genus Bradyrhizobium consistent with results of DNA–DNA hybridization. Based on analysis of symbiosis gene sequences (nodC and nifH), all novel strains were placed in a phylogenetic group with five species of the genus Bradyrhizobium that nodulate soybeans. The combination of phenotypic characteristics from several tests including carbon and nitrogen source utilization and antibiotic resistance could be used to differentiate representative strains from recognized species of the genus Bradyrhizobium. Novel strain OO99T elicits effective nodules on Glycine max, Glycine soja and Macroptilium atropurpureum, partially effective nodules on Desmodium canadense and Vigna unguiculata, and ineffective nodules on Amphicarpaea bracteata and Phaseolus vulgaris. Based on the data presented, we conclude that our strains represent a novel species for which the name Bradyrhizobium ottawaense sp. nov. is proposed, with OO99T ( = LMG 26739T = HAMBI 3284T) as the type strain. The DNA G+C content is 62.6 mol%.

show abstract

“…The subcommittee earlier suggested that the word rhizobium (plural rhizobia) can be used as a common name for legumenodulating, nitrogen-fixing bacteria, irrespective of genus (Lindström and Martinez-Romero, 2005, Int J Syst Evol Microbiol 55, 1383 DOI 10.1099/ijs.0.63744-0), and further distinction of alpha-rhizobia and beta-rhizobia for rhizobia affiliated Bradyrhizobium cytisi CTAW11 Cytisus villosus [35] Bradyrhizobium huanghuaihaiense HAMBI 3180 Glycine max [36] Bradyrhizobium oligotrophicum ATCC 43045 Rice paddy soil [37] Bradyrhizobium daqingense CCBAU 15774 Glycine max [38] Bradyrhizobium diazoefficiens USDA 110 Glycine max [39] Bradyrhizobium arachidis CCBAU 051107 Arachis hypogaea [40] Aminobacter Aminobacter anthyllidis STM4645 Anthyllis vulneraria [41] de Lajudie and Martinez-Romero, Int J Syst Evol Microbiol 2017;67:516-520…”

Section: Minute 8 Legume-nodulating Bacterial Species Outside Rhizobiummentioning

confidence: 99%

International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Agrobacterium and Rhizobium Minutes of the meeting, 7 September 2014, Tenerife, Spain

Lajudie

Martı́nez-Romero

2017

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Bradyrhizobium arachidis sp. nov., isolated from effective nodules of Arachis hypogaea grown in China

Cited by 84 publications

References 25 publications

Genetic Diversity and Evolution of Bradyrhizobium Populations Nodulating Erythrophleum fordii, an Evergreen Tree Indigenous to the Southern Subtropical Region of China

Genetic Diversity and Evolution of Bradyrhizobium Populations Nodulating Erythrophleum fordii, an Evergreen Tree Indigenous to the Southern Subtropical Region of China

Bradyrhizobium ottawaense sp. nov., a symbiotic nitrogen fixing bacterium from root nodules of soybeans in Canada

International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Agrobacterium and Rhizobium Minutes of the meeting, 7 September 2014, Tenerife, Spain

Contact Info

Product

Resources

About