Bradyrhizobia associated with Laburnum anagyroides, an exotic legume grown in Poland

Abstract

We isolated 18 rhizobial strains from root nodules of a leguminous shrub Laburnum anagyroides (common laburnum) grown in Southeast Poland as an exotic plant. With the use of BOX-PCR fingerprinting, the isolates were clustered into 2 main groups and one separate lineage, which was congruent with the ITS-RFLP results. The phylogenetic trees constructed based on 16S rRNA and combined atpD, dnaK, glnA, and recA gene sequence data separated the representative strains into three evolutionary lineages within the Bradyrhizobium jicamae supergroup, with Bradyrhizobium algeriense and Bradyrhizobium valentinum as the closest relatives. The nodA and nifH gene phylogenies proved that the L. anagyroides symbionts carry a symbiotic gene variant known as Clade IV, representing the symbiovar retamae. Phenotypic characteristics of the isolates and reference strains are also reported. Our study of the rhizobia nodulating L. anagyroides growing in Poland complements earlier few findings on the symbiotic associations of this Genisteae species.

Structure of root nodules in <i>Laburnum anagyroides</i> Medik.

“…However, none of the strains fully effective with the native genistean species and lupine were able to induce any nodules in L. anagyroides in this study. It was not possible to identify the filtrate-contained rhizobia that nodulated L. anagyroides -albeit ineffectively -in this study, but in a different analysis of strains isolated from L. anagyroides in Poland, rhizobia compatible with the species were discovered (Sajnaga & Jach, 2020). Among 18 effective strains, three evolutionary lineages were determined that belonged to the Bradyrhizobium jicamae supergroup, symbiovar retamae, with Bradyrhizobium algeriense and Bradyrhizobium valentinum as the closest relatives.…”

“…However, in an earlier study conducted within the geographical range of L. anagyroides, its Spanish microsymbionts were identified as Bradyrhizobium canariense (Ruiz-Díez et al, 2009). Significantly, five of rhizobial strains obtained by Sajnaga and Jach (2020) were isolated from L. anagyroides grown in a botanical garden, i.e., in a place where numerous foreign species are cultivated for a long time, and therefore the soil microbiome is, most probably, much more modified and richer than typically. All strains were isolated from locations in Lublin and Sandomierz, which differ in soils and weather conditions from Central Poland.…”

Genotypic Variation of Endophytic Nitrogen-Fixing Activity and Bacterial Flora in Rice Stem Based on Sugar Content

“…Bootstrap values of ≥50% are indicated at the respective nodes. Reference type strains are indicated with superscript “†.” The backgrounds of the reference strains are colored according to the sugar (sucrose and/or glucose) utilization capacity of those bacteria based on the data from (1) Poindexter ( 2015 ); (2) Hamzah et al ( 2006 ); (3) Felföldi et al ( 2019 ); (4) Sajnaga and Jach ( 2020 ); (5) Kuykendall et al ( 2015 ); (6) Sheu et al ( 2020 ); (7) Xie and Yokota ( 2006 ); (8) Raj et al ( 2012 ); (9) Weon et al ( 2008 ); (10) Kennedy ( 2015 ); (11) Anandham et al ( 2009 ); (12), Noar and Buckley ( 2009 ); (13) Imhoff ( 2015a ); (14) Hardoim et al ( 2013 ); (15) Grimont and Grimont ( 2015 ); (16) Lee et al ( 2020 ); (17) Normand and Benson ( 2015 ); (18) Stackebrandt and Kandler ( 1979 ); (19) Holt et al ( 1988 ); (20), Rainey et al ( 2015 ); (21) Janssen ( 2015 ); (22) Okamura et al ( 2011 ); (23) Thrash and Coates ( 2015 ). The black-and-white shading in the columns on the right-hand of the 16S-OTU name indicates the mean relative abundance of the 16S-OTUs in each rice genotype ( n = 2 for Nipponbare and CG14; n = 3 for agpl1 and Leafstar).…”

“…Bootstrap values of ≥50% are indicated at their respective nodes. Reference type strains are indicated with superscript “†.” The backgrounds of the reference strains are colored according to the sugar (sucrose and/or glucose) utilization capacity of those bacteria based on the data from (1) Lee et al ( 2013 ); (2) Staley ( 1984 ); (3) Kuykendall et al ( 2015 ); (4) Dall'Agnol et al ( 2013 ); (5) Baldani et al ( 2015 ); (6) Hungria et al ( 1993 ); (7) Kuykendall ( 2015 ); (8) Sajnaga and Jach ( 2020 ); (9) Ahnia et al ( 2018 ); (10) Reis et al ( 2004 ); (11) Chen et al ( 2006 ); (12) Coenye et al ( 2001 ); (13) Wolterink et al ( 2005 ); (14) Weon et al ( 2008 ); (15) Qiu et al ( 2017 ); (16) Hardoim et al ( 2013 ); (17) Xu et al ( 2020 ); (18) Ueki et al ( 2006 ); (19) Olsen ( 2015 ); (20) Imhoff ( 2015b ); (21) Diaz-Barrera et al ( 2016 ); (22) Bae et al ( 2006 ); (23) Norris et al ( 2015 ); and (24) Patrick and McDowell ( 2015 ). The black-and-white shading in the columns on the right-hand of the NifH-OTU name indicates the mean relative abundance of the NifH-OTUs in each rice genotype ( n = 2 for Nipponbare; n = 3 for agpl1 , Leafstar, and CG14).…”

“…Ma-8, B. diazoefficiens USDA 110 † , and Bradyrhizobium sp. MN-S) used sucrose, glucose, and fructose (Hameed et al, 2004;Dall'Agnol et al, 2013;Sajnaga and Jach, 2020) (Figures 6, 8). Paraburkholderia mimosarum PAS44 † (97-99% identity with NifH-OTUI10-13) can also metabolize glucose and fructose (Chen et al, 2006) (Figure 8).…”