Bradykinin and cardiovascular system: estimation of half-life

Saameli, K; Eskes, T.K.A.B.

doi:10.1152/ajplegacy.1962.203.2.261

Cited by 72 publications

(28 citation statements)

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“…It was possible to apply bradykinin repeatedly at a dose of 100 pmol in intervals of 10 min showing that there is no development of tolerance to this dose. The effect only lasted for approximately 1 min, confirming the short halflife of bradykinin in blood, calculated to be less than 30s in man (Saameli & Eskes, 1962), in the dog (McCarthy et al, 1965) and in the cat (Ferreira & Vane, 1967b). An i.a.…”

Section: Agonistic and Antagonistic Propertiessupporting

confidence: 57%

Effects of the bradykinin antagonist B4310 on smooth muscles and blood pressure in the rat, and its enzymatic degradation

Griesbacher

Lembeck

Saria

1989

British J Pharmacology

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Austria1 Six competitive bradykinin (Bk) antagonists were tested for their agonistic properties on the rat uterus. Five of these peptides showed agonistic effects only at concentrations at least two orders of magnitude higher than those of bradykinin. 2 The antagonistic potency of Lys-Lys-3-Hyp-5,8-Thi-7-DPhe-Bk (B4310) in the rat uterus (pA2 = 7.24) and in the rat duodenum (pA2 = 7.31) was very similar to that determined in an earlier study for the antagonism of the bradykinin-induced stimulation of the trigeminal nerve in the rabbit iris sphincter muscle preparation (pA2 = 7.59). 3 The fall in mean arterial blood pressure induced by i.a. injections of bradykinin was greatly reduced during an i.a. infusion of B4310, but not 10min thereafter, which indicates a rapid inactivation of B4310 in,vivo. Bacitracin possibly interferes with the enzymatic cleavage of B4310 but seems to have no effect on the degradation of bradykinin. 4 An i.a. infusion of captopril greatly enhanced the potency of bradykinin in inducing a fall in arterial blood pressure, confirming the important role of angiotensin converting enzyme in the cleavage of bradykinin. However, the design of this experiment did not allow conclusions about the effect of captopril on the degradation of B43 10. 5 B4310 incubated with rat lung tissue disappeared from the incubation medium within a few minutes, i.e. as fast as bradykinin, which explains its short duration of action in vivo. Captopril partially inhibited the cleavage of both bradykinin and B4310. 6 The present results show that the bradykinin antagonists available at present are useful tools for the investigation of the biological role of bradykinin. However, the susceptibility to enzymatic degradation may limit their usefulness in animal experiments or in clinical studies.

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Section: Agonistic and Antagonistic Propertiessupporting

confidence: 57%

Effects of the bradykinin antagonist B4310 on smooth muscles and blood pressure in the rat, and its enzymatic degradation

Griesbacher

Lembeck

Saria

1989

British J Pharmacology

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“…The rise in kininogen levels during pregnancy reported by Periti & Gasparri (1966) (Saameli & Eskes, 1962).…”

Section: Discussionmentioning

confidence: 90%

“…It now seems likely that the activity is due, at least in part, to the prostaglandins (Karim, 1969), but the belief that polypeptides may contribute to it has led several workers to study the precursors of the plasma kinins (kininogens) in the blood in order to determine the extent to which they are mobilized in pregnancy and labour. Kininogen rather than kinin levels have been estimated because, until recently, it has not been possible to determine the free kinin levels in plasma with any degree of accuracy, since rapid enzyme action gives an extremely short half-life to the kinins, both in vivo and in vitro (Saameli & Eskes, 1962). Martinez, Carvalho & Diniz (1962) found that the kininogen content of plasma fell during labour but returned to prepartum values in the puerperium.…”

Section: Introductionmentioning

confidence: 99%

Plasma Kinin and Kininogen Levels in Women During Pregnancy and in Labour

Porter¹,

Shennan²,

Smith³

1972

Reproduction

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Summary. Kininogen and kinin levels in venous plasma were determined in groups of normal adult men and women and in pregnant women at different periods of gestation and in labour.Kininogen levels were found to be significantly higher in the pregnant women than in the male and female controls. A significant positive linear correlation was found between period of gestation and plasma kininogen level.Differences between the mean plasma kinin levels in groups of control adults and pregnant women were in most cases not significant.No significant linear correlation was found between kinin and kininogen levels in plasma in any of the groups studied and no definite trends were found in the kinin and kininogen levels at delivery.

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“…The half-life of synthetic bradykinin in the body is less than 05 min (Saameli & Eskes, 1962;McCarthy, Potter & Nicolaides, 1965), and kinin activity is easily lost during purification. The inadvertent production of kinins during sampling and the separation of plasma is an even greater hazard.…”

Section: Estimation Of Kinins In Man Discussionmentioning

confidence: 99%

Determination of plasma kinin and kininogen levels in man

Brocklehurst¹,

Zeitlin²

1967

The Journal of Physiology

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SUMMARY1. A method for the estimation of free plasma kinin and kininogen is described, which is suitable for samples of blood taken in hospital.2. The method permits the assay of very low levels of kinin and substantially eliminates errors due to the presence of amines and other interfering substances.3. In normal subjects the mean value of free kinin is 2-8 ng (bradykinin equivalent)/ml. plasma, and 6-1,ug kininogen/ml.4. In vasovagal fainting, carcinoid flush, and dumping syndrome, during the phase of peripheral vasodilatation, the free kinin exceeds 10 ng/ml., and is often in excess of 30 ng/ml.5. Sudden release of free kinin is accompanied by a fall in kininogen level.

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Bradykinin and cardiovascular system: estimation of half-life

Cited by 72 publications

References 0 publications

Effects of the bradykinin antagonist B4310 on smooth muscles and blood pressure in the rat, and its enzymatic degradation

Effects of the bradykinin antagonist B4310 on smooth muscles and blood pressure in the rat, and its enzymatic degradation

Plasma Kinin and Kininogen Levels in Women During Pregnancy and in Labour

Determination of plasma kinin and kininogen levels in man

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