Bract suppression regulated by the miR156/529-SPLs-NL1-PLA1 module is required for the transition from vegetative to reproductive branching in rice

Wang, Lei; Ming, Luchang; Liao, Keyan; Xia, Chen; Sun, Shengyuan; Chang, Yu Sun; Wang, Hongkai; Fu, Debao; Xu, Conghao; Wang, Zhengji; Xu, Li; Xie, Weibo; Ouyang, Yidan; Zhang, Qinglu; Li, Xianghua; Xiao, Jinghua

doi:10.1016/j.molp.2021.04.013

Cited by 41 publications

(31 citation statements)

References 95 publications

(152 reference statements)

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“…Previous research has demonstrated that OsSPL14 is a target gene of miR156 and miR529 (Wang et al., 2015, 2021; Xie et al., 2006). In this study, enhanced miR529/miR156 expression also led to a bushy phenotype caused by the accelerated outgrowth of axillary buds in the transgenic plants (Figure S2).…”

Section: Resultsmentioning

confidence: 99%

“…OsSPL14 , also known as IDEAL PLANT ARCHITECTURE1 ( IPA1 ) or WEALTHY FARMER'S PANICLE ( WFP ), is a plant‐specific transcription factor encoding gene targeted by miR156 and miR529 in rice (Chen et al., 2015; Jiao et al., 2010; Miura et al., 2010; Wang et al., 2015, 2021; Xie et al., 2006; Yan et al., 2021). A number of studies have demonstrated that miR156/miR529 and OsSPL14 participate in various processes of rice, such as seedling growth (He et al., 2021; Miao et al., 2019), disease resistance (Liu et al., 2019; Wang et al., 2018b), drought tolerance (Zhu et al., 2022), bract outgrowth (Wang et al., 2021), root elongation (Sun et al., 2019, 2021), and architecture foundation (Duan et al., 2019a; Gou et al., 2017; Jiao et al., 2010; Liu et al., 2015b; Miura et al., 2010; Wang et al., 2015, 2021; Wang et al., 2017b; Wang et al., 2017a; Zhang et al., 2017). Recently, OsSPL14 was found positively regulated by CIRCADIAN CLOCK ASSOCIATED1 (OsCCA1), which integrates the sugar response and the SL pathway to balance tiller bud and panicle development (Wang et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

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OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine‐tuning auxin transport in rice

Liu

et al. 2022

The Plant Journal

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SUMMARY As a multigenic trait, rice tillering can optimize plant architecture for the maximum agronomic yield. SQUAMOSA PROMOTER BINDING PROTEIN‐LIKE14 (OsSPL14) has been demonstrated to be necessary and sufficient to inhibit rice branching, but the underlying mechanism remains largely unclear. Here, we demonstrated that OsSPL14, which is cleaved by miR529 and miR156, inhibits tillering by fine‐tuning auxin transport in rice. RNA interference of OsSPL14 or miR529 and miR156 overexpression significantly increased the tiller number, whereas OsSPL14 overexpression decreased the tiller number. Histological analysis revealed that the OsSPL14‐overexpressing line had normal initiation of axillary buds but inhibited outgrowth of tillers. Moreover, OsSPL14 was found to be responsive to indole‐acetic acid and 1‐naphthylphthalamic acid, and RNA interference of OsSPL14 reduced polar auxin transport and increased 1‐naphthylphthalamic acid sensitivity of rice plants. Further analysis revealed that OsSPL14 directly binds to the promoter of PIN‐FORMED 1b (OsPIN1b) and PIN‐LIKE6b (PILS6b) to regulate their expression positively. OsPIN1b and PILS6b were highly expressed in axillary buds and proved involved in bud outgrowth. Loss of function of OsPIN1b or PILS6b increased the tiller number of rice. Taken together, our findings suggested that OsSPL14 could control axillary bud outgrowth and tiller number by activating the expression of OsPIN1b and PILS6b to fine‐tune auxin transport in rice.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine‐tuning auxin transport in rice

Liu

et al. 2022

The Plant Journal

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“…As shown in Figure 3 E, some TE-derived tissue-specific ACRs were located at the upstream of subsets of functional tissue-specific genes. For example, a MULE -derived ACR was located in the promoter of a hydroquinone glucosyltransferase encoding gene ( LOC_Os03g44180 ), preferentially expressed in root (RT); a hAT transposon related ACR was located near the TSS of NL1 with higher expression levels in young panicle (YP), which is a GATA type transcription factor related to the panicle development [ 38 ]; a Gypsy retrotransposon-derived ACR was located in the upstream region of OsBISAMT1 , a gene encoding S-adenosyl-L-methionine related to defense responses, which was specially expressed in young leaf (YL) [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Transposon-Derived Accessible Chromatin Regions in Rice (Oryza Sativa)

Zhang

2022

IJMS

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Growing evidence indicates that transposons or transposable elements (TEs)-derived accessible chromatin regions (ACRs) play essential roles in multiple biological processes by interacting with trans-acting factors. However, the function of TE-derived ACRs in the regulation of gene expression in the rice genome has not been well characterized. In this study, we examined the chromatin dynamics in six types of rice tissues and found that ~8% of ACRs were derived from TEs and exhibited distinct levels of accessibility and conservation as compared to those without TEs. TEs exhibited a TE subtype-dependent impact on ACR formation, which can be mediated by changes in the underlying DNA methylation levels. Moreover, we found that tissue-specific TE-derived ACRs might function in the tissue development through the modulation of nearby gene expression. Interestingly, many genes in domestication sweeps were found to overlap with TE-derived ACRs, suggesting their potential functions in the rice domestication. In addition, we found that the expression divergence of 1070 duplicate gene pairs were associated with TE-derived ACRs and had distinct distributions of TEs and ACRs around the transcription start sites (TSSs), which may experience different selection pressures. Thus, our study provides some insights into the biological implications of TE-derived ACRs in the rice genome. Our results imply that these ACRs are likely involved in the regulation of tissue development, rice domestication and functional divergence of duplicated genes.

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“…Regulatory modules consisting of miRNAs and target genes have been reported in response to disease resistance and abiotic stresses in plants. Some common targeting OsSPL genes exist in miR156/529 that have important roles in regulating inflorescence structure and seed yield in rice [ 58 ]. For instance, panicle architecture and grain size were controlled by miR529a through altering the expression of all five target genes OsSPL2 , OsSPL7 , OsSPL14 , OsSPL16 , OsSPL17 and OsSPL18 [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

OsSPLs Regulate Male Fertility in Response to Different Temperatures by Flavonoid Biosynthesis and Tapetum PCD in PTGMS Rice

Sun

Wang

et al. 2022

IJMS

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Photoperiod and thermo-sensitive genic male sterile (PTGMS) rice is an important resource for two line hybrid rice production. The SQUAMOSA–promoter binding, such as the (SPL) gene family, encode the plant specific transcription factors that regulate development and defense responses in plants. However, the reports about SPLs participating in male fertility regulation are limited. Here, we identified 19 OsSPL family members and investigated their involvement in the fertility regulation of the PTGMS rice lines, PA2364S and PA2864S, with different fertility transition temperatures. The results demonstrated that OsSPL2, OsSPL4, OsSPL16 and OsSPL17 affect male fertility in response to temperature changes through the MiR156-SPL module. WGCNA (weighted gene co-expression network analysis) revealed that CHI and APX1 were co-expressed with OsSPL17. Targeted metabolite and flavonoid biosynthetic gene expression analysis revealed that OsSPL17 regulates the expression of flavonoid biosynthesis genes CHI, and the up regulation of flavanones (eriodictvol and naringenin) and flavones (apigenin and luteolin) content contributed to plant fertility. Meanwhile, OsSPL17 negatively regulates APX1 to affect APX (ascorbate peroxidase) activity, thereby regulating ROS (reactive oxygen species) content in the tapetum, controlling the PCD (programmed cell death) process and regulating male fertility in rice. Overall, this report highlights the potential role of OsSPL for the regulation of male fertility in rice and provides a new insight for the further understanding of fertility molecular mechanisms in PTGMS rice.

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Bract suppression regulated by the miR156/529-SPLs-NL1-PLA1 module is required for the transition from vegetative to reproductive branching in rice

Cited by 41 publications

References 95 publications

OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine‐tuning auxin transport in rice

OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine‐tuning auxin transport in rice

Characterization of Transposon-Derived Accessible Chromatin Regions in Rice (Oryza Sativa)

OsSPLs Regulate Male Fertility in Response to Different Temperatures by Flavonoid Biosynthesis and Tapetum PCD in PTGMS Rice

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