Brachyury in the gastrula of basal vertebrates

Bruce, Ashley E.E.; Winklbauer, Rudolf

doi:10.1016/j.mod.2020.103625

Cited by 15 publications

(15 citation statements)

References 189 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The significant downregulation of tbxt in most embryos argues for the second possibility ( Fig. 4 S-U), which suffices to explain the observed problems of tissue remodeling, axial elongation and notochord morphogenesis, since Tbxt is a well-known upstream regulator of mesoderm specification and non-canonical Wnt pathways required for CE ( Bruce and Winklbauer, 2020 ; Schulte-Merker and Smith, 1995 ; Tada and Smith, 2000 ).…”

Section: Discussionmentioning

confidence: 93%

Rab7 is required for mesoderm patterning and gastrulation in Xenopus

2021

View full text Add to dashboard Cite

Early embryogenesis requires tightly controlled temporal and spatial coordination of cellular behavior and signaling. Modulations are achieved at multiple levels, from cellular transcription to tissue-scale behavior. Intracellularly, the endolysosomal system emerges as an important regulator at different levels – but in vivo studies are rare. In the frog Xenopus, little is known about developmental roles of endosomal regulators, or their potential involvement in signaling, especially true for late endosomes. Here, we chose to analyze a hypothesized role of Rab7 in this context, a small GTPase known for its role as a late endosomal regulator. First, rab7 showed strong maternal expression. Following localized zygotic transcript enrichment in the mesodermal ring and neural plate, it was found in tailbud stage neural ectoderm, notochord, pronephros, eyes and neural crest tissues. Inhibition resulted in strong axis defects caused by a requirement of rab7 for mesodermal patterning and correct gastrulation movements. To test a potential involvement in growth factor signaling, we analyzed early Wnt-dependent processes in the mesoderm. Our results suggest a selective requirement for ligand-induced Wnt activation, implicating a context-dependent role of Rab7.

show abstract

Section: Discussionmentioning

confidence: 93%

Rab7 is required for mesoderm patterning and gastrulation in Xenopus

2021

View full text Add to dashboard Cite

show abstract

“…IHC is another efficient method to visually reveal the location and abundance of the candidate proteins in three germ layers in situ. As a transcription factor, T has a role in the formation and organization of mesoderm and is regarded as a traditional mesoderm marker 19,54,55 . Two other candidate genes, Fn1 and Cdh2 , which express at relatively high abundances and are located mainly at the membrane, were selected to further validate their location in three germ layers on embryo cryosections with limited cell numbers.…”

Section: Resultsmentioning

confidence: 99%

“…As a transcription factor, T has a role in the formation and organization of mesoderm and is regarded as a traditional mesoderm marker. 19 , 54 , 55 Two other candidate genes, Fn1 and Cdh2 , which express at relatively high abundances and are located mainly at the membrane, were selected to further validate their location in three germ layers on embryo cryosections with limited cell numbers. As shown in Figure 3D , in the middle of the E7.5 intraembryonic region, T, was specifically expressed at the cell nucleus of the primitive streak cells; FN1 was shown to express on the membrane outlining the mesoderm that separated it from the ectoderm and the endoderm, and CDH2 is specifically expressed on the membrane of the mesoderm (Figure 3D ).…”

Section: Resultsmentioning

confidence: 99%

Spatial transcriptomic profiling to identify mesoderm progenitors with precision genomic screening and functional confirmation

et al. 2022

View full text Add to dashboard Cite

Objectives: Mesoderm, derived from a new layer between epiblast and hypoblast during gastrulation, can differentiate into various tissues, including muscles, bones, kidneys, blood, and the urogenital system. However, systematic elucidation of mesoderm characteristics and specific markers remains a challenge. This study aims to screen and identify candidate genes important for mesoderm development.Materials and Methods: Cells originating from the three germ layers were obtained by laser capture microdissection, followed by microcellular RNA sequencing. Mesoderm-specific differentially expressed genes (DEGs) were identified by using a combination of three bioinformatics pipelines. Candidate mesoderm-specific genes expression were verified by real-time quantitative polymerase chain reaction analysis and immunohistochemistry. Functional analyses were verified by ESCs-EBs differentiation and colony-forming units (CFUs) assay.Results: A total of 1962 differentially expressed mesoderm genes were found, out of which 50 were candidate mesoderm-specific DEGs which mainly participate in somite development, formation of the primary germ layer, segmentation, mesoderm development, and pattern specification process by GO analysis. Representative genes Cdh2, Cdh11, Jag1, T, Fn-1, and Pcdh7 were specifically expressed in mesoderm among the three germ layers. Pcdh7 as membrane-associated gene has hematopoietic-relevant functions identified by ESCs-EBs differentiation and CFUs assay.Conclusions: Spatial transcriptomic profiling with multi-method analysis and confirmation revealed candidate mesoderm progenitors. This approach appears to be efficient and reliable and can be extended to screen and validate candidate genes in various cellular systems.Guanghui Liu and Guanheng Yang contributed equally to this study.

show abstract

“…The significant down-regulation of tbxt in most embryos might argue for the second possibility ( Fig. 3P-R), which could explain all observed problems of tissue remodeling, CE and notochord morphogenesis -as Tbxt is a well-known upstream regulator of mesoderm specification and non-canonical Wnt pathways required for CE (Bruce and Winklbauer, 2020;Schulte-Merker and Smith, 1995;Tada and Smith, 2000).…”

Section: Rab7 Is Required For Dorsal Mesoderm Specification and Tissumentioning

confidence: 99%

Rab7 mediates Wnt-dependent mesoderm patterning and gastrulation inXenopus

Kreis

Wielath

Vick

2020

Preprint

View full text Add to dashboard Cite

Early embryogenesis requires tightly controlled temporal and spatial activity of growth factors. Modulation is achieved at multiple levels, from cellular transcription to tissue-scale propagation, concerning activation, maintenance, or termination of signaling. Intracellularly, the endolysosomal system emerged as important factor for such regulations. Endocytosis and early endosomes mainly orchestrate activation and transduction of signals, while late endosomes are thought to mediate lysosome-based termination. Only few reports attributed activating roles to late endosomes, mainly for Wnt pathway activation. In Xenopus, few is known about developmental roles of endosomal regulators, or their function for signaling, especially true for late endosomes. Therefore, we chose to analyze a hypothesized positive role of Rab7 in this context, a small GTPase mainly known for its role as a late endosomal regulator. We analyzed if Rab7 participates in early activation of growth factor signaling, focusing on Wnt signaling. We found rab7 expressed very localized throughout development, and inhibition resulted in strong gastrulation defects. Concomitantly, Rab7 was strictly required for both, exogenous activation and endogenous Wnt-mediated patterning of the mesoderm.

show abstract

Brachyury in the gastrula of basal vertebrates

Cited by 15 publications

References 189 publications

Rab7 is required for mesoderm patterning and gastrulation in Xenopus

Rab7 is required for mesoderm patterning and gastrulation in Xenopus

Spatial transcriptomic profiling to identify mesoderm progenitors with precision genomic screening and functional confirmation

Rab7 mediates Wnt-dependent mesoderm patterning and gastrulation inXenopus

Contact Info

Product

Resources

About