2016
DOI: 10.1038/srep25510
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Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust

Abstract: Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most destructive diseases and can cause severe yield losses in many regions of the world. Because of the large size and complexity of wheat genome, it is difficult to study the molecular mechanism of interaction between wheat and PST. Brachypodium distachyon has become a model system for temperate grasses’ functional genomics research. The phenotypic evaluation showed that the response of Brachypodium distachyon to PST was no… Show more

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Cited by 23 publications
(14 citation statements)
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“…Although there are over 25 000 T-DNA lines, only 31% of the genes (about 8000) were reportedly tagged (Hsia et al, 2017). Additionally, a separate T-DNA collection of 7000 B. distachyon lines was developed to identify mutants that compromise nonhost resistance against wheat stripe rust pathogen (An et al, 2016). Here we report that the tobacco retrotransposon Tnt1 can transpose in B. distachyon during somatic embryogenesis and can be used as a mutagen to achieve saturation or near-saturation mutagenesis.…”
Section: Discussionmentioning
confidence: 99%
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“…Although there are over 25 000 T-DNA lines, only 31% of the genes (about 8000) were reportedly tagged (Hsia et al, 2017). Additionally, a separate T-DNA collection of 7000 B. distachyon lines was developed to identify mutants that compromise nonhost resistance against wheat stripe rust pathogen (An et al, 2016). Here we report that the tobacco retrotransposon Tnt1 can transpose in B. distachyon during somatic embryogenesis and can be used as a mutagen to achieve saturation or near-saturation mutagenesis.…”
Section: Discussionmentioning
confidence: 99%
“…Insertion mutagenesis based on T-DNA is also popularly used in plants such as Arabidopsis (Alonso et al, 2003), rice (Jeon et al, 2000) and, more recently, B. distachyon (An et al, 2016;Hsia et al, 2017). A total of 21 165 B. distachyon T-DNA lines were sequenced as part of the Joint Genome Institute sequencing efforts, bringing the total of unique insertion sites to 26 112 (Hsia et al, 2017).…”
Section: Introductionmentioning
confidence: 99%
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“…This region contains about 40 annotated genes, and there is no previously annotated host or non-host resistance gene in the interval. Recently, three genes involved in Brachypodium NHR to Pst were identified by flanking sequence isolation from mutant (An et al, 2016). Among them, Bradi5g17540 encodes a BAP28 domain containing protein, Bradi5g17540 encodes a MYB transcription factor and Bradi5g11590 encodes a lipoxygenase.…”
Section: Discussionmentioning
confidence: 99%
“…There are different ways for selection and screening of transgenic calli by using reporter genes like uidA or gfp (An et al 2016). Also, both HPT (hygromicin B resistance) and BAR genes (Basta®/ Bialaphos resistance) can be used as selectable markers (Thole and Vain 2012;Steinwand et al 2013;Bragg et al 2012Bragg et al , 2015.…”
Section: Introductionmentioning
confidence: 99%