BPH1, a novel substrate receptor of CRL3, plays a repressive role in ABA signal transduction

Woo, Og Geum; Kim, Soon Hee; Cho, Seok Keun; Kim, Sang Hoon; Lee, Han Nim; Chung, Taijoon; Yang, Seong Wook; Lee, Jae Hoon

doi:10.1007/s11103-018-0717-x

Cited by 11 publications

(8 citation statements)

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“…Stomatal aperture analysis was performed as previously described by Woo et al (2018). Leaves of 5‐week‐old plants were floated in buffer containing 10 mM MES‐KOH (pH 6.15), 50 mM KCl, and 10 mM CaCl 2 for 2.5 h in the light.…”

Section: Methodsmentioning

confidence: 99%

Loss‐of‐function of ARABIDOPSIS F‐BOX PROTEIN HYPERSENSITIVE TO ABA 1 enhances drought tolerance and delays germination

Kim

Song

Kim

et al. 2021

Physiologia Plantarum

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ABA is a phytohormone involved in diverse plant events such as seed germination and drought response. An F‐box protein functions as a substrate receptor of the SCF complex and is responsible for ubiquitination of target proteins, triggering their subsequent degradation mediated by ubiquitin proteasome system. Here, we have isolated a gene named ARABIDOPSIS F‐BOX PROTEIN HYPERSENSITIVE TO ABA 1 (AFA1) that was upregulated by ABA. AFA1 interacted with adaptor proteins of the SCF complex, implying its role as a substrate receptor of the complex. Its loss of function mutants, afa1 seedlings, exhibited ABA‐hypersensitivity, including delayed germination in the presence of ABA. Moreover, loss of AFA1 led to increased drought tolerance in adult plants. Microarray data with ABA treatments indicated that 129 and 219 genes were upregulated or downregulated, respectively, by more than three times in afa1 relative to the wild type. Among the upregulated genes in afa1, the expression of 28.7% was induced by more than three times in the presence of ABA, while only 9.3% was repressed to the same extent. These data indicate that AFA1 is involved in the downregulation of many ABA‐inducible genes, in accordance with the ABA‐hypersensitive phenotype of afa1. Epistasis analysis showed that AFA1 could play a role upstream of ABI4 and ABI5 in the ABA signaling for germination inhibition. Collectively, our findings suggest that AFA1 is a novel F‐box protein that negatively regulates ABA signaling.

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Section: Methodsmentioning

confidence: 99%

Loss‐of‐function of ARABIDOPSIS F‐BOX PROTEIN HYPERSENSITIVE TO ABA 1 enhances drought tolerance and delays germination

Kim

Song

Kim

et al. 2021

Physiologia Plantarum

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“…NRL3 has recently been identified to interact with CUL3A through its BTB domain as well, and was renamed BPH1 (BTB/POZ PROTEIN HYPERSENSITIVE TO ABA 1) due to the nature of the mutant phenotype in response to the plant hormone abscisic acid (ABA) (Woo et al, 2018). While no role in blue light signaling has yet been identified for NRL3/BPH1, Woo and colleagues (2018) speculate that the presence of the NPH3 domain in this gene family member may indeed imply the potential.…”

Section: Nph3 Along With Rootphototropism2 (Rpt2) Are Founding Members Of the Nph3-rpt2-like (Nrl) 33-member Protein Familymentioning

confidence: 99%

“…Furthermore, two members of this family: NRL31/NCH1 (NRL PROTEIN FOR CHLOROPLAST MOVEMENT 1)/ SR1IP1 (AtSR1 interaction protein 1) and NRL3/ BPH1 (BTB/POZ PROTEIN HYPERSENSITIVE TO ABA 1) have both been shown to interact with CUL3 through the BTB domain (Zhang et al, 2014;Woo et al, 2018).…”

Section: Chapter Characterization Of Phot1 Function In Mutants With Altered Phot1 Ubiquitination Introductionmentioning

confidence: 99%

“…(NRL PROTEIN FOR CHLOROPLAST MOVEMENT 1)/ SR1IP1 (AtSR1 interaction protein 1) have been shown to interact with CUL3 and act as a substrate adapter in an E3 ubiquitin ligase complex (Suetsugu et al, 2016;Zhang et al, 2014;Woo et al, 2018) with NRL31/NCH1/SR1IP1 functioning in chloroplast movement, which is another phot-mediated function (Suetsugu et al, 2016).…”

Section: Chapter 3 Characterizing An Allelic Series Of Mutants Within Nph3 To Identify Domain Function Introductionmentioning

confidence: 99%

“…Most interesting is the involvement of RPT2 and NRL31/NCH1 (NRL PROTEIN FOR CHLOROPLAST MOVEMENT 1)/ SR1IP1 (AtSR1 interaction protein 1) in phot1-mediated chloroplast accumulation (Suetsugu et al, 2016), as well as the fact that both these proteins interact with phot1 (Inada et al, 2004;Suetsugu et al, 2016). Furthermore, NRL31/NCH1/SR1IP1 as well as recently identified NRL3/ BPH1 (BTB/POZ PROTEIN HYPERSENSITIVE TO ABA 1), have been shown to interact with CUL3 to form E3 ubiquitin ligase complexes (Woo et al, 2018;Zhang et al, 2014…”

Section: Chapter 4 Conclusion and Future Directions Introductionmentioning

confidence: 99%

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Mechanisms of the phototropic pathway

Morrow¹

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Plants are sessile organisms and therefore are limited in their ability to gather resources. Therefore, they have evolved several mechanisms that aid them in their quest to gather light, water and nutrients. One such mechanism is phototropism, a plants ability to bend towards or away from a light source. This mechanism is mediated by the blue light photoreceptor phototropin (phot). Arabidopsis thaliana contains two phototropins, phot1 which is the primary photoreceptor under low intensity light and phot2, which acts redundantly with phot1 under high intensity light. The perception of blue light by the phototropins (phot1 and phot2) initiates signaling events that lead to a lateral redistribution of the plant hormone auxin; which ultimately results in differential growth and the bending response. In addition to phototropism, these proteins mediate several other growth and developmental responses such as leaf movement, chloroplast movement, and stomatal opening. A second protein which is critical to the phototropic response and interacts with phot1 is NONPHOTOTROPIC HYPOCOTYL3 (NPH3). In addition to phototropism, NPH3 has a role in phot1-mediated leaf movement. NPH3 has been shown to act as a substrate adapter in an E3 ubiquitin ligase complex with the protein CULLIN3 (CUL3). This CRL3NPH3 complex is responsible for ubiquitinating the phot1 photoreceptor in a blue light fashion. This ubiquitination has been shown to be necessary for the bending response, but it's role in receptor ubiquitination is still not fully understood. To better understand this component of phot1-mediated phototropism, we characterized an allelic series of NPH3 mutants to further understand the role of this substrate adapter in this mechanism. Additionally, we characterized several mutant plant lines containing a mutant phot1 protein in which critical ubiquitination sites were mutated in an effort to render the protein unable to be ubiquitinated to further understand phot1 ubiquitination.

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Identification of a conserved set of cytokinin-responsive genes expressed in the fruits of Prunus persica

et al. 2020

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BPH1, a novel substrate receptor of CRL3, plays a repressive role in ABA signal transduction

Cited by 11 publications

References 68 publications

Loss‐of‐function of ARABIDOPSIS F‐BOX PROTEIN HYPERSENSITIVE TO ABA 1 enhances drought tolerance and delays germination

Loss‐of‐function of ARABIDOPSIS F‐BOX PROTEIN HYPERSENSITIVE TO ABA 1 enhances drought tolerance and delays germination

Mechanisms of the phototropic pathway

Identification of a conserved set of cytokinin-responsive genes expressed in the fruits of Prunus persica

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