BPDE, the Migration and Invasion of Human Trophoblast Cells, and Occurrence of Miscarriage in Humans: Roles of a Novel
            <i>lncRNA-HZ09</i>

Dai, Mengyuan; Huang, Weijian; Huang, Xinying; Ma, Chenglong; Wang, Rong; Tian, Peng; Chen, Weina; Zhang, Ying; Mi, Chenyang; Zhang, Huidong

doi:10.1289/ehp10477

Cited by 24 publications

(25 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells with log-growth period were seeded into 24-well plates. The actinomycin D (5 mg/ml) [ 13 ] was added after cell starvation for 24 h. Cells were harvested at 0 h, 0.5 h, 1 h, 4 h, 8 h and 12 h in the 37℃ 5% CO 2 cell incubator and RNA was extracted. Then lncRNA stability was determined by RT-qPCR assay.…”

Section: Methodsmentioning

confidence: 99%

LncRNA-BC069792 suppresses tumor progression by targeting KCNQ4 in breast cancer

et al. 2023

View full text Add to dashboard Cite

Background Breast cancer is the most common malignant tumor that threatens women's health. Attention has been paid on the study of long- non-coding RNA (lncRNA) in breast cancer. However, the specific mechanism remains not clear. Methods In this study, we explored the role of lncRNA BC069792 in breast cancer. In vitro and in vivo functional experiments were carried out in cell culture and mouse models. High-throughput next-generation sequencing technology and real-time fluorescence quantitative PCR technology were used to evaluate differentially expressed genes and mRNA expression, Western blot and immunohistochemical staining were used to detect protein expression. RNA immunoprecipitation assay and dual-luciferase activity assay were used to evaluate the competing endogenous RNAs (ceRNA), and rescue and mutation experiments were used for verification. Results We found that lncRNA BC069792 was expressed at a low level in breast cancer tissues, and significantly decreased in breast cancer with high pathological grade, lymph node metastasis and high Ki-67 index groups. Moreover, BC069792 inhibited the proliferation, invasion and metastasis of breast cancer cells in vitro and in vivo. Mechanically, BC069792 acts as a molecular sponge to adsorb hsa-miR-658 and hsa-miR-4739, to up-regulate the protein expression of Potassium Voltage-Gated Channel Q4 (KCNQ4), inhibits the activities of JAK2 and p-AKT, and plays a role in inhibiting breast cancer growth. Conclusions LncRNA BC069792 plays the role of tumor suppressor gene in breast cancer and is a new diagnostic index and therapeutic target in breast cancer.

show abstract

Section: Methodsmentioning

confidence: 99%

LncRNA-BC069792 suppresses tumor progression by targeting KCNQ4 in breast cancer

et al. 2023

View full text Add to dashboard Cite

show abstract

“… 1 Now, experimental research in Environmental Health Perspectives sheds light on how one ubiquitous pollutant, benzo(a)pyrene [B(a)P], might elevate risk for recurrent miscarriage. 5 …”

mentioning

confidence: 99%

“…A by-product of incomplete combustion, B(a)P is a polyaromatic hydrocarbon (PAH) found in tobacco and wood smoke, car exhaust, and grilled foods, among other sources. 6 According to the new study, 5 maternal exposure to B(a)P increased the expression of certain genes and proteins that may interfere with the attachment of a very early stage embryo—called a blastocyst—to the uterine wall. The research was led by Huidong Zhang, a professor at the Eighth Affiliated Hospital, Sun Yat-sen University, in Shenzhen, China.…”

mentioning

confidence: 99%

“… 7 – 9 During the new study, Zhang and colleagues focused specifically on developmental effects of the ultimate metabolite of B(a)P: benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE). 5 Previous research by this team and others showed that BPDE inhibits the normal biology of cells called trophoblasts, which form the outer layer of the blastocyst and give rise to the placenta. 10 , 11 For the developing embryo to establish a blood supply, trophoblasts must first migrate to and then invade the endometrial lining of the uterus.…”

mentioning

confidence: 99%

“…Zhang and colleagues focused specifically on how BPDE disrupts the activity of long noncoding RNAs (lncRNAs), 5 which have key roles in trophoblast functioning. In an earlier cell study, the team identified 22 lncRNAs that are more active in human trophoblasts after BPDE exposure.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Failure to Connect: Experimental Evidence that Benzo(a)pyrene Impedes Embryonic Implantation

Schmidt¹

2023

Environ Health Perspect

View full text Add to dashboard Cite

Defective Homologous Recombination Repair By Up‐Regulating Lnc‐HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage

Chen,

Mi,

Zhang

et al. 2024

Advanced Science

Self Cite

View full text Add to dashboard Cite

Human trophoblast cells are crucial for healthy pregnancy. However, whether the defective homologous recombination (HR) repair of dsDNA break (DSB) in trophoblast cells may induce miscarriage is completely unknown. Moreover, the abundance of BRCA1 (a crucial protein for HR repair), its recruitment to DSB foci, and its epigenetic regulatory mechanisms, are also fully unexplored. In this work, it is identified that a novel lnc‐HZ10, which is highly experssed in villous tissues of recurrent miscarriage (RM) vs their healthy control group, suppresses HR repair of DSB in trophoblast cell. Lnc‐HZ10 and AhR (aryl hydrocarbon receptor) form a positive feedback loop. AhR acts as a transcription factor to promote lnc‐HZ10 transcription. Meanwhile, lnc‐HZ10 also increases AhR levels by suppressing its CUL4B‐mediated ubiquitination degradation. Subsequently, AhR suppresses BRCA1 transcription; and lnc‐HZ10 (mainly 1‐447 nt) interacts with γ‐H2AX; and thus, impairs its interactions with BRCA1. BPDE exposure may trigger this loop to suppress HR repair in trophoblast cells, possibly inducing miscarriage. Knockdown of murine Ahr efficiently recovers HR repair in placental tissues and alleviates miscarriage in a mouse miscarriage model. Therefore, it is suggested that AhR/lnc‐HZ10/BRCA1 axis may be a promising target for alleviation of unexplained miscarriage.

show abstract

BPDE, the Migration and Invasion of Human Trophoblast Cells, and Occurrence of Miscarriage in Humans: Roles of a Novel lncRNA-HZ09

Cited by 24 publications

References 64 publications

LncRNA-BC069792 suppresses tumor progression by targeting KCNQ4 in breast cancer

LncRNA-BC069792 suppresses tumor progression by targeting KCNQ4 in breast cancer

Failure to Connect: Experimental Evidence that Benzo(a)pyrene Impedes Embryonic Implantation

Defective Homologous Recombination Repair By Up‐Regulating Lnc‐HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage

Contact Info

Product

Resources

About