Box-Behnken Design Based Statistical Modeling and Optimization of Cellulase-Assisted Microwave Extraction of Polysaccharide from Elaeagnus Angustifolia L.

Abstract

Cellulaseassisted microwave extraction of polysaccharides from Elaeagnus angustifolia L. was researched. The four influential parameters, extraction temperature, pH value, microwave extraction power and microwave time, were optimized using the Box-Behnken design (BBD) with a quadratic regression model built by using response surface methodology (RSM). As results, the extraction conditions have significant effects on extraction yield of polysaccharide, a quadratic model was found to fit for EAP yield, and the optimal conditions was determined as following: extraction temperature of 38°C,pH value of 5.0,microwave power of 509W and irradiation time of 60 s. A maximum yield of 19.57± 0.02% for EAP was achieved in verification experiment.

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“…RSM provides both the experimental design and the process optimisation based on the mathematical and statistical model. The Box-Behnken Design (BBD) and Central Composite Design (CCD) are the most common and suitable for the process optimisation [93][94][95][96][97][98]. However, BBD is more well known than CCD for the ability to predict and determine the optimal condition in the least number of experiments [99][100][101][102][103][104].…”