Bovine Whole Blood Cells as a Biomarker Platform for Biological Toxicology: A Focus on Thiacloprid

Galdíková, Martina; Holečková, Beáta; Schwarzbacherová, Viera

doi:10.1007/978-3-031-07392-2_35

Cited by 1 publication

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“…These bind to the receptor of the neurotransmitter acetylcholine, thereby blocking it and consequently causing the increased stimulation and paralysis of the insect's nervous system. The properties of neonicotinoids, such as good water solubility and long persistence and accumulation in the environment, are associated with undesirable cytotoxic and genotoxic impacts on nontarget organisms [12].…”

Section: -Methylcytosine (5mcmentioning

confidence: 99%

Evaluation of GSTP1, GSTA4 and AChE Gene Methylation in Bovine Lymphocytes Cultured In Vitro with Miconazole Alone and in Combination with Mospilan 20SP

Halušková,

Holečková,

Schwarzbacherová

et al. 2023

Genes

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5-methylcytosine (5mC) is one of the most important epigenetic modifications. Its increased occurrence in regulatory sequences of genes, such as promoters and enhancers, is associated with the inhibition of their expression. Methylation patterns are not stable but are sensitive to factors such as the environment, diet, and age. In the present study, we investigated the effects of fungicide miconazole, both alone and in combination with the insecticide Mospilan 20SP, on the methylation status of bovine GSTP1, GSTA4, and AChE genes in bovine lymphocytes cultured in vitro. The methylation-specific PCR technique was used for the objectives of this study. We found that miconazole alone at concentrations of 1.25, 2.5, 5, 10, 25, and 50 µg/mL after 24 h exposure probably did not induce changes in methylation for all three genes analysed. The same results were found for the combination of pesticides at 24 h exposure and the following concentrations for each of them: 0.625, 1.25, 2.5, 5, and 12.5 µg/mL. Thus, we can conclude that the fungicide miconazole alone, as well as in combination with the insecticide Mospilan 20SP, was unlikely to cause changes to the methylation of bovine GSTP1, GSTA4, and AChE genes.

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Section: -Methylcytosine (5mcmentioning

confidence: 99%