Bovine Vibriosis in Two Herds of Beef Cattle in Northern Queensland

Donaldson, Lloyd E.; Clark, B. L.

doi:10.1111/j.1751-0813.1970.tb09174.x

Cited by 8 publications

(9 citation statements)

References 3 publications

(4 reference statements)

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“…Screening with known pathogenicity island (PAI) genes .......... 143 3.2. Screening of type IV secretion system (T4S) genes B6 (Donaldson and Clark, 1970), 642-21 (Newsam and Peterson, 1964) and ATCC19438 in PBS. Table 1.…”

Section: Fields Of Research (For) Classificationmentioning

confidence: 99%

“…Table 1. Characterisation of catalase-positive Campylobacter strains (Véron and Châtelain, 1973) 28 (Donaldson and Clark, 1970), 642-21 (Newsam and Peterson, 1964) Table 22. New D-mycarose 3-C-methyltransferase real-time assay primers and probe sequences.…”

Section: Fields Of Research (For) Classificationmentioning

confidence: 99%

“…Correlation of cell and CFU counts with D-mycarose 3-C-methyltransferase, parA and ISCfe1 real-time Ct scores obtained from C. fetus subsp. venerealis reference strains B6 (Donaldson and Clark, 1970), 642-21 (Newsam and Peterson, 1964) (BonDurant, 2005;Clark, 1971). BGC can cause important economic impact to beef exporting countries while international guidelines require a "certification of disease free status" to export both bulls and semen (BonDurant, 2005;Campero, 2000;McCool et al, 1988b;OIE, 2012b;Swai et al, 2005).…”

Section: Fields Of Research (For) Classificationmentioning

confidence: 99%

“…cultures were sourced from BGC cases (Donaldson and Clark, 1970;Newsam and Peterson, 1964), from Biosecurity Queensland (DAFF), from the American Type Culture Collection (ATCC), and from the National Collection of Type Cultures (NCTC, Table 7). …”

Section: Reference Culturesmentioning

confidence: 99%

“…venerealis reference strains B6 (Donaldson and Clark, 1970) and 642-21 (Newsam and Peterson, 1964) and ATCC19438 (Table 7) were diluted 1:10 in formalin to decrease cell motility. Bacterial cell counts were performed in duplicates using an improved Neubauer haemocytometer.…”

Section: Sensitivity Comparisonmentioning

confidence: 99%

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Molecular diagnostic protocols for bovine genital campylobacteriosis using comparative genomics and virulence studies

Indjein¹

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Bovine genital campylobacteriosis is difficult to identify due to problems with the isolation of its causative agent Campylobacter fetus subspecies venerealis. To optimise isolation methods, three selective culture and three transport and enrichment media (TEMs) were evaluated using a pure C. fetus subsp. venerealis culture, with and without simulated field contamination by Pseudomonas aeruginosa. For optimal C. fetus subsp. venerealis isolation and growth, TEMs should be returned from the field within 48h of collection and plated within 4h onto Campylobacter selective agar plates incubated for 48-72h at 37°C under microaerophilic conditions. Longer transport or TEM incubation resulted in overgrowth of P. aeruginosa limiting C. fetus subsp. venerealis recovery.In order to establish an isolate collection, the parA real-time PCR assay was used to screen bull preputial secretions prior to culturing positive samples. A more sensitive real-time PCR assay was designed from the ISCfe1 insertion element and used to assess additional preputial samples, compared to the parA real-time assay. A proportion of real-time negative samples was also cultured. Fourteen cultures were obtained from either ISCfe1 (8/43; 18.6%) or parA (6/51; 11.8%) negative samples. ISCfe1 and parA sequences were obtained from isolates not confirmed by conventional methods suggesting that both parA and ISCfe1 may be found in organisms other than C. fetus subsp. venerealis. Molecular testing of cultures (n=84) confirmed the presence of these sequences in isolates identified as other Campylobacter spp. or Arcobacter-like organisms, while they were not always detected in all biochemically confirmed C. fetus subsp. venerealis isolates. The lack of specificity of all methods highlights the need for more reliable C. fetus subsp. venerealis-specific gene targets.The draft genomes of two C. fetus subsp. venerealis biovars aligned to C. fetus subsp. fetus genome provided 26 unique C. fetus subsp. venerealis regions. In order to identify new diagnostic targets, 13 putative conventional and one real-time PCR assays designed from contigs common to both C. fetus subsp. venerealis biovars were developed to screen the Campylobacter-like culture collection (n=84). Isolates biochemically identified as organisms other than C. fetus subsp.venerealis were positive on the new real-time and six conventional PCR assays, indicating that they were not suitable subspecies-specific markers. Seven assays detected isolates phenotyped as C. fetus subsp. venerealis except for two aerotolerant isolates recognized as C. fetus subsp. venerealis by iii other published methods. These seven gene targets could be further investigated for real-time PCR assay development as in silico BLAST analysis confirmed their conservation in the genomes of five of our C. fetus subsp. venerealis isolates. However, further bioinformatics analysis revealed that they were found in several C. fetus subsp. fetus strains, underlining that a pan-genomics study including multiple C. fetus genomes is ne...

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Section: Fields Of Research (For) Classificationmentioning

confidence: 99%