Bovine umbilical vein endothelial cells conditioned medium prevents TMT-induced neurotoxicity mediated by the upregulation of brain-derived neurotropic factors on the human neuroblastoma SH-SY5Y cells

Abstract

Background: Neurodegenerative diseases are characterized by damage to the central nervous system through the progressive decrease in the structure and function of neurons, followed by neuronal death. Current therapy for these diseases is still limited. Bovine umbilical vein endothelial cells conditioned medium (BUVEC-CM) is a medium harvested from culture of bovine umbilical vein endothelial cells (BUVEC). This study aimed to evaluate the capability of BUVEC-CM to prevent neurotoxicity induced by trimethyltin (TMT) on human neuroblastoma SH-SY5Y cells. Methods: The tests carried out were a viability test with MTT and CCK-8 assay, scratch wound assay to determine cell migration, Hoechst 33342 and AO/PI staining to detect apoptosis, ELISA test to measure the concentration of BDNF, and RT-qPCR to determine the expression of CD68, caspase-7 and caspase-9, and DCFH-DA staining to detect reactive oxygen species (ROS). Results: BUVEC-CM maintained the viability of cells, increase cell migration, prevent cell apoptosis, increase BDNF levels, decrease the expression of CD68, as well as the caspase-7 and -9 genes, and suppress ROS generation. Conclusions: BUVEC-CM can protect from neurodegeneration in SH-SY5Y cells through upregulation of BDNF and downregulation of CD68, caspase-7 and caspase-9.