Bovine sponge amnion stimulates socket healing: A histological analysis

Faadhila, Tabita Imaniar; Valentina, Martha Nadila; Munadziroh, Elly; Nirwana, Intan; Soekartono, Helal; Surboyo, Meircurius Dwi Condro

doi:10.4103/japtr.japtr_128_20

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…Increasing vascular permeability increased endothelial cell proliferation, endothelial cell mitosis, controlled endothelial cell migration, created new blood vessel lumens and macrophage chemotaxis, and vasodilation, all of which are goals of new blood vessel development. 33,34 The increase in the number of blood vessels and fibroblasts occurred due to the synergistic effect of the active compounds in the purple leaves extract. It was known that purple leaves extract contains active compounds such as alkaloids, flavonoids, saponins, and tannins.…”

Section: Discussionmentioning

confidence: 99%

The role of purple leaves extract (Graptophyllum Pictum (L.) Griff) on the number of fibroblasts and blood vessels in the socket after tooth extraction

Kurniawati,

Kristanti,

Rahmat

et al. 2024

Dent. J.

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Background: Tooth extraction is the process of removing teeth from the alveolus. It will leave a mark on the socket and surrounding soft tissue. One of the cells that plays an important role in the wound healing process after tooth extraction is fibroblasts. When an injury occurs, some blood vessels are damaged; therefore, new blood vessels need to form. Purple leaves extract could be an alternative treatment for wound healing after tooth extraction as it contains flavonoids, saponins, alkaloids, steroids, and tannins. Purpose: The study aimed to analyze the role of purple leaves extract on the increase in fibroblasts and blood vessels in the socket after tooth extraction in Wistar rats. Methods: The method used was a laboratory experiment with a post-test-only control group design. The samples used were 24 rats divided into two groups: the control group, which was given aquadest, and the treatment group, which was given 1.5 mL of purple leaves extract with 10% concentration by sondage. Tissue preparations were used to count fibroblasts, and blood vessels were counted and observed on the 3rd, 5th, and 7th days. Results: Statistical tests showed a significant difference in the number of fibroblasts and blood vessels between the control and treatment groups on days 3, 5, and 7. Conclusion: Purple leaves extract could increase the number of fibroblasts and blood vessels in the tooth socket after tooth extraction of Wistar rats.

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Section: Discussionmentioning

confidence: 99%

The role of purple leaves extract (Graptophyllum Pictum (L.) Griff) on the number of fibroblasts and blood vessels in the socket after tooth extraction

Kurniawati,

Kristanti,

Rahmat

et al. 2024

Dent. J.

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“…TGF-β and FGF stimulate fibroblast activity. 35 All of these growth factors are highly essential during all phases of wound healing. However, since most of them are growth factors, if TGF-β is overexpressed it can lead to fibrosis and hypertrophic scar formation.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Application Bovine Amniotic Membrane on Osteoblasts, Osteocytes, and Collagen

Wibowo,

Octarina,

Munadziroh

et al. 2023

Padjadjaran J Dent

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Introduction: Tooth extraction can result in loss of alveolar bone. The application of biomaterials Bovine Amniotic Membrane (BAM) that containing growth factors and collagen is expected to play a role in socket preservation. This study aimed to observe the effect of BAM application on the growth of osteoblasts, osteocytes, and collagen in histological preparate of the alveolar bone socket of Sprague Dawley rats (SD). Methods: 16 male SD rats were used in this group. SD rats were extracted on the mandible central incisivus. Afterward, it was divided into two groups, specifically the control group (without treatment) and the treatment group (BAM application). After extraction, the treatment group was applied with BAM with a size of 1.5 mm x 10 mm in socket alveolar bone. On the 14th and 28th day, the rats were euthanized and the alveolar bone socket of mandibular tissue was taken and stained with Hematoxylin Eosin (HE) and Masson Trichrome (MT). Results: The results of all groups showed that the number of osteoblasts and collagen thickness was higher on day 14th compared to day 28th, while osteocytes continued to increase from day 14th to 28th. The thickness of collagen, osteoblasts, and osteocytes in groups with BAM application was higher than without application. Statistical results showed that there were significant differences between groups (p<0.05). Conclusion: The application of BAM can act as material preservation socket. This biomaterial can accelerate recovery by increasing collagen thickness, osteoblasts, and osteocytes.

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“…BAM can be found in bovine placentas, and shares a chemical composition resembling the human bone. 5,6 With its potential for tissue repair and regeneration, BAM accelerates re-epithelialization and wound healing. BAM exhibits osteoinductive properties and antibacterial effects due to antimicrobial peptides such as defensin, elafin, and SLPI (Secretory Leukocyte Protease Inhibitor).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and cytotoxic effects of fresh bovine amniotic membrane with hydroxyapatite (BAM-HA): a laboratory experiment

Octarina,

Berliana,

Kalangit

2024

Padjadjaran J Dent

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Introduction: Bacterial infections, particularly by Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and Porphyromonas gingivalis (P. gingivalis), can worsen alveolar bone resorption after tooth extraction. The capability of Bovine Amniotic Membrane-Hydroxyapatite (BAM-HA) biocomposite to reduce this resorption has been explored. However, before clinical use, cytotoxicity testing is imperative to ensure its biocompatibility. The aim of the study was to assess both the antibacterial effects and cytotoxicity of the BAM-HA biocomposite to ensure its suitability for clinical use biocompatibility of the BAM-HA biocomposite before its clinical application. Methods: The laboratory-based research involved testing BAM combined with HA powder in 4:1 and 4:2 ratios via freeze-drying and underwent antibacterial tests against A. actinomycetemcomitans and P. gingivalis, using the plate count method. Cytotoxicity tests were performed on HGF cells, including negative control, positive control, BAM-HA (4:1), and BAM-HA (4:2) groups, with statistical analysis conducted using One-Way ANOVA and Post Hoc Bonferroni and Tukey tests. Results: Antibacterial tests against A. actinomycetemcomitans revealed significant reduction in colony count with BAM-HA ratios 4:1 (129.0 ± 12.7 CFU/mL) and 4:2 (77.3 ± 15.5 CFU/mL) compared to the negative control (186.6 ± 27.5 CFU/mL). Similar reductions were observed for P. gingivalis, with BAM-HA ratios 4:1 (51.3 ± 6.6 CFU/mL) and 4:2 (3.1 ± 1.5 CFU/mL) compared to the negative control (117.3 ± 22.0 CFU/mL). Cytotoxicity tests showed no significant differences in HGF cell viability and IC50 values between the negative control and BAM-HA (4:1) or BAM-HA (4:2) groups. Conclusion: The BAM - HA biocomposite shows antibacterial effects against A. actinomycetemcomitans and P. gingivalis. Moreover, BAM - HA ratios of 4:1 and 4:2 do not induce cytotoxic effects on human gingival fibroblasts, suggesting potential biocompatibility for clinical applications.KeywordA. actinomycetemcomitans, antibacterial effects, BAM-HA biocomposite, cytotoxicity, P. gingivalis

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Bovine sponge amnion stimulates socket healing: A histological analysis

Cited by 5 publications

References 9 publications

The role of purple leaves extract (Graptophyllum Pictum (L.) Griff) on the number of fibroblasts and blood vessels in the socket after tooth extraction

The role of purple leaves extract (Graptophyllum Pictum (L.) Griff) on the number of fibroblasts and blood vessels in the socket after tooth extraction

The Effect of Application Bovine Amniotic Membrane on Osteoblasts, Osteocytes, and Collagen

Antibacterial and cytotoxic effects of fresh bovine amniotic membrane with hydroxyapatite (BAM-HA): a laboratory experiment

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