Bovine sperm chromatin is not protected from the effects of ultrasmall gold nanoparticles

Zakhidov, S. T.; Pavlyuchenkova, S. M.; Samoylov, A. V.; Mudzhiri, N. M.; Маршак, Т. Л.; Rudoy, V. M.; Дементьева, О. В.; Zelenina, I. A.; Skuridin, S. G.; Yevdokimov, Yu. M.

doi:10.1134/s1062359013060149

Cited by 17 publications

(11 citation statements)

References 37 publications

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“…Similar effects could be detected in epidydimal sperms in adult rats after exposure to AgNPs [67], even if the exposure occurs during the prepubertal stage [70]. These effects are also evident in bull sperms [71] and are likely to be due to direct interaction with the sperm cells; since in bulls, AuNPs were shown to impair sperm functions and reduce sperm fertilizing ability, solely by interaction with the sperm surface membrane, and without cellular penetration [72].…”

Section: Controversies In the Use Of Nanotechnology In Reproduction: mentioning

confidence: 53%

Perspectives of nanotechnology in male fertility and sperm function

Falchi

Khalil

Hassan³

et al. 2018

International Journal of Veterinary Science and Medicine

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Recent advances in nanotechnology have tremendously expanded its possible applications in biomedicine. Although, the effects of nanoparticles (NPs) at cellular and tissue levels have not been fully understood, some of these biological effects might be employed in assisted reproduction to improve male fertility particularly by enhancing sperm cell quality either in vivo or in vitro. This review summarises the available literature regarding the potential applications of nanomaterials in farm animal reproduction, with a specific focus on the male gamete and on different strategies to improve breeding performances, transgenesis and targeted delivery of substances to a sperm cell. Antioxidant, antimicrobial properties and special surface binding ligand functionalization and their applications for sperm processing and cryopreservation have been reviewed. In addition, nanotoxicity and detrimental effects of NPs on sperm cells are also discussed due to the increasing concerns regarding the environmental impact of the expanding use of nanotechnologies on reproduction.

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Section: Controversies In the Use Of Nanotechnology In Reproduction: mentioning

confidence: 53%

Perspectives of nanotechnology in male fertility and sperm function

Falchi

Khalil

Hassan³

et al. 2018

International Journal of Veterinary Science and Medicine

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“…In the mouse, CeO 2 NPs induce DNA damage in both oocytes and spermatozoa affecting in vitro fertilization [ 14 ] whereas in the ovine species, supplementation of maturation media with CeO 2 NPs (44μg/mL) did not impair but enhanced fertilization and blastocyst rate and no adverse effects were observed in chromatin configuration of oocytes exposed to NPs [ 15 ]. On the other side chromatin damage has been reported in mice [ 40 ] and bull spermatozoa [ 41 ] exposed to silver NPs.…”

Section: Discussionmentioning

confidence: 99%

Effect of exposure to CeO2 nanoparticles on ram spermatozoa during storage at 4 °C for 96 hours

Falchi

Galleri

Dore

et al. 2018

Reprod Biol Endocrinol

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BackgroundCerium oxide nanoparticles (CeO2 NPs) are able to store and release oxygen, conferring them scavenger activity against oxidative stress. However, their effects in reproductive systems are not yet well understood. The aim of the study was to investigate the effects of exposure of refrigerated ram semen to CeO2 NPs for 96 h on the main structural and kinematic parameters of spermatozoa.MethodsThe ejaculates of 5 Sarda rams were collected, pooled and diluted in a soybean lecithin extender. Samples were exposed to increasing doses of CeO2 NPs (0, 44 and 220 μg/mL) and stored at 4 °C for 96 h. Analyses of kinematic parameters (computer assisted sperm analysis, CASA), integrity of membranes (PI/PSA staining), ROS production (H2DCFDA staining) and DNA damage (sperm chromatin structure assay with acridine orange, SCSA) were performed every 24 h (0, 24, 48, 72 and 96 h of incubation). The experiment was carried out in 6 replicates. Data were analysed by repeated measures ANOVA with Bonferroni’s as post hoc test. When the assumption of normality was not met (ROS), non-parametric Kruskal-Wallis rank test was carried out.ResultsExposure of ram spermatozoa to increasing doses of CeO2 NPs had a beneficial effect on the main motility parameters from 48 h of incubation onward. Velocity of sperm cells was enhanced in the groups exposed to CeO2 NPs compared to the control. Incubation with NPs had beneficial effects on the integrity of plasma membranes of spermatozoa, with higher percentage of damaged cells in the control group compared to the exposed ones. Production of ROS was not affected by exposure to NPs and its levels rose at 96 h of incubation. The integrity of DNA remained stable throughout the 96 h of storage regardless of co-incubation with NPs.ConclusionsWe reported beneficial effects of CeO2 NPs on kinematic and morphologic parameters of ram semen, such as motility and membrane integrity following 96 h of exposure. Furthermore, we also proved no genotoxic effects of CeO2 NPs. These effects could not be related to an antioxidant activity of CeO2 NPs, since ROS levels in exposed cells were similar to those of unexposed ones.

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“…115 Fourth, this work 115 has stimulated intensive studies of Russian scientists (see, e.g., Ref. 59) not only confirming the nano-Au effect on spermatogenesis but also demonstrating a diversity of possible mechanisms of nano-Au action on animals. This is also supported by studies.…”

Section: Iv7 Change In Absorption Spectrum Caused By the Effect Of mentioning

confidence: 73%

Physicochemical and nanotechnological approaches to the design of 'rigid' spatial structures of DNA

Yevdokimov¹,

Salyanov²,

Skuridin³

et al. 2015

Russ. Chem. Rev.

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In recent years, many studies have focused on the design of new spatial structures based on linear double-stranded (ds) DNA molecules considered as a functional polymer (see, e.g., 1, 2 ). However, DNA hydrogels rather than spatial structures are often used in practice. 3 Double-stranded DNA hydrogels can be obtained, in particular, through creation of artificial cross-links between ds-DNA molecules, 4 formation of complexes with polyvalent cations (Ca 2+ , Mg 2+ , Co 2+ , Ni 2+ , Fe 2+ , Al 3+ ), 5, 6 cryopolymerization 7 or exposure to UV light. The ds-DNA hydrogels are macroscopic assemblies of irregularly crosslinked and disorderly arranged ds-DNA molecules. In hydrogels, ds-DNA molecules retain many of their inherent Yu M Yevdokimov Doctor of Chemical Sciences, Professor, Chief Researcher of the IMB RAS. Telephone: (7-499) 135 97 20, e-mail: yevdokim@eimb.ru V I Salyanov Candidate of Chemical Sciences, Senior Researcher of the same institute. Telephone: (7-499) 135 97 20, e-mail: vsalyanov@yandex.ru S G Skuridin Doctor of Biological Sciences, Senior Researcher of the same institute. Telephone: (7-499) 135 97 20, e-mail: lancet-bio@bk.ru E V Shtykova Candidate of Biological Sciences, Senior Researcher of the IC RAS. Telephone: (7-499) 135 40 20,

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Bovine sperm chromatin is not protected from the effects of ultrasmall gold nanoparticles

Cited by 17 publications

References 37 publications

Perspectives of nanotechnology in male fertility and sperm function

Perspectives of nanotechnology in male fertility and sperm function

Effect of exposure to CeO2 nanoparticles on ram spermatozoa during storage at 4 °C for 96 hours

Physicochemical and nanotechnological approaches to the design of 'rigid' spatial structures of DNA

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