Bovine Skeletal Muscle Cells Predominantly Express a Vascular Cell Adhesion Molecule-1 Seven-Ig Domain Splice Form

Muroya, Susumu; Nakajima, Ikuyo; Chikuni, Koichi

doi:10.2108/zsj.18.797

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…Bovine muscle cells were also readily able to proliferate and differentiate without necessitating a specific differentiation medium which was required in previous reported primary bovine muscle cultures [ 11 , 29 , 38 , 39 ]. After 3 days of seeding in 24 well plates, bovine muscle cells underwent terminal differentiation and fusion into extensive and large multi-nucleated myotubes (Figure 5 e-h).…”

Section: Resultsmentioning

confidence: 99%

A simplified but robust method for the isolation of avian and mammalian muscle satellite cells

et al. 2012

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BackgroundCurrent methods of isolation of muscle satellite cells from different animal species are highly variable making inter-species comparisons problematic. This variation mainly stems from the use of different proteolytic enzymes to release the satellite cells from the muscle tissue (sometimes a single enzyme is used but often a combination of enzymes is preferred) and the different extracellular matrix proteins used to coat culture ware. In addition, isolation of satellite cells is frequently laborious and sometimes may require pre-plating of the cell preparation on uncoated flasks or Percoll centrifugation to remove contaminating fibroblasts. The methodology employed to isolate and culture satellite cells in vitro can critically determine the fusion of myoblasts into multi-nucleated myotubes. These terminally differentiated myotubes resemble mature myofibres in the muscle tissue in vivo, therefore optimal fusion is a keystone of in vitro muscle culture. Hence, a simple method of muscle satellite cell isolation and culture of different vertebrate species that can result in a high fusion rate is highly desirable.ResultsWe demonstrate here a relatively simple and rapid method of isolating highly enriched muscle satellite cells from different avian and mammalian species. In brief, muscle tissue was mechanically dissociated, digested with a single enzyme (pronase), triturated with a 10-ml pipette, filtered and directly plated onto collagen coated flasks. Following this method and after optimization of the cell culture conditions, excellent fusion rates were achieved in the duck, chicken, horse and cow (with more than 50% cell fusion), and to a lesser extent pig, pointing to pronase as a highly suitable enzyme to release satellite cells from muscle tissue.ConclusionsOur simplified method presents a quick and simple alternative to isolating highly enriched muscle satellite cell cultures which can subsequently rapidly differentiate into well developed primary myotubes. The use of the same isolation protocol allows better inter-species comparisons of muscle satellite cells. Of all the farm animal species investigated, harvested chicken muscle cells showed the highest percentage of muscle satellite cells, and equine muscle cells presented the highest fusion index, an impressive ≈ 77%. Porcine cells displayed the lowest amount of satellite cells but still achieved a modest fusion rate of ≈ 41%.

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Section: Resultsmentioning

confidence: 99%

A simplified but robust method for the isolation of avian and mammalian muscle satellite cells

et al. 2012

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“…The cell preparation was performed according to the method originally designed by Nara et al. (2000), followed by some modification (Muroya et al. 2001a,b).…”

Section: Methodsmentioning

confidence: 99%

“…Because the mRNA sample before day 9 in culture was omitted as the number of cells available was too few, the expression pattern of MRFs from cell preparation to day 9 in culture was not investigated. First‐strand cDNA was synthesized from 0.5 μg of the total RNA using the M‐MLV Reverse Transcriptase RNase‐H minus (Toyobo, Tokyo, Japan) with the primer 3ADP1 (Muroya et al. 2001a).…”

Section: Methodsmentioning

confidence: 99%

Sequential expression of myogenic regulatory factors in bovine skeletal muscle and the satellite cell culture

Muroya

Nakajima

Chikuni

2002

Animal Science Journal

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Myogenic regulatory factors (MRFs) are important in the control of skeletal muscle development. To understand myogenic regulation by MRFs in bovine adult muscle cells, their expressions, namely that of Myf5, MyoD, myogenin, and MRF4 in the biceps femoris muscle (BF) and in the satellite cell culture, were analyzed by RT-PCR. In the BF, all four MRFs were expressed and in particular, myogenin and MRF4 were strongly expressed, whereas Myf5 was faintly expressed. The satellite cells prepared from the BF expressed Myf5, but only a trace of MyoD, at day 9 of culture. During the growth of the cells to day 14, the MyoD and myogenin expressions gradually increased, and that of MyoD expression reached its maximum at the confluence of the culture. After induction of myogenic differentiation by a serum-free medium at day 14, Myf5 expression gradually decreased, and the up-regulated expression of MyoD was suppressed, whereas myogenin expression continued to increase sharply. Following the myogenin expression, MRF4 also drastically increased toward the myotube formation of the cells. When huge myotubes were formed at day 18, Myf5 was expressed at a low level, whereas the MyoD expression remained at a moderate level.

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“…Bovine satellite cells (BSCs) were used to create the first clean meat prototype (Post, 2014), and their growth in bioreactors for clean meat production was recently assessed (Verbruggen et al, 2018). BSCs can be isolated from bovine carcasses (Dodson et al, 1987;Frey et al, 1995;Muroya et al, 2001;Kamanga-Sollo et al, 2004, 2008Ding et al, 2018), biopsies (Frey et al, 1995) or fetuses (Bridge et al, 1998;. The muscle tissue is ground and enzymatically digested using pronase, collagenase II or trypsin.…”

Section: Satellite Cells and Myogenesismentioning

confidence: 99%

Tissue Engineering for Clean Meat Production

Ben-Arye

Levenberg

2019

Front. Sustain. Food Syst.

188

134

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Increasing public awareness of foodborne illnesses, factory farming, and the ecological footprint of the meat industry, has generated the need for animal-free meat alternatives. In the last decade, scientists have begun to leverage the knowledge and tools accumulated in the fields of stem cells and tissue engineering toward the development of cell-based meat (i.e., clean meat). In tissue engineering, the physical and biochemical features of the native tissue can be mimicked; cells and biomaterials are integrated under suitable culture conditions to form mature tissues. More specifically, in skeletal muscle tissue engineering, a plurality of cell types can be co-cultured on a 3D scaffold to generate muscle fibers, blood vessels and a dense extracellular matrix (ECM). This review focuses on tissue engineering of skeletal muscle and the adjustments needed for clean meat development. We discuss the skeletal muscle structure and composition, and elaborate on cell types from farm animals that have the potential to recapitulate the muscle ECM, blood vessels, muscle fibers and fat deposits. We also review relevant biomaterials, primarily for fabricating scaffolds that can mimic the intramuscular connective tissues, as well as gene expression studies on the biological pathways that influence meat quality.

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Bovine Skeletal Muscle Cells Predominantly Express a Vascular Cell Adhesion Molecule-1 Seven-Ig Domain Splice Form

Cited by 8 publications

References 51 publications

A simplified but robust method for the isolation of avian and mammalian muscle satellite cells

A simplified but robust method for the isolation of avian and mammalian muscle satellite cells

Sequential expression of myogenic regulatory factors in bovine skeletal muscle and the satellite cell culture

Tissue Engineering for Clean Meat Production

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