Bovine Skeletal Muscle Adenosine Deaminase:
Kinetic and Thermodynamic Studies

Martínez, Castro; Jm, Zumalacárregui; Diez,

doi:10.1159/000469119

Cited by 2 publications

(1 citation statement)

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“…Likewise, with a kcat of 375 s −1 and a kcat/Km of 1.4 ×10 7 M −1 s −1 , ADA catalysis is close to being encounter‐limited . Given its apparent simplicity and catalytic efficiency, ADA has been the focus of extensive investigations in an effort to understand its substrate specificity and enzymatic stability, the kinetic mechanism and the Michaelis–Menten parameters of the ADA reaction, the action of different inhibitors, and the comparison of several physicochemical properties of the enzyme from different sources …”

Section: Ada As An Enzyme Structural and Catalytic Characteristicsmentioning

confidence: 99%

Moonlighting Adenosine Deaminase: A Target Protein for Drug Development

Cortés

Gracia

Moreno

et al. 2014

Medicinal Research Reviews

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Interest in adenosine deaminase (ADA) in the context of medicine has mainly focused on its enzymatic activity. This is justified by the importance of the reaction catalyzed by ADA not only for the intracellular purine metabolism, but also for the extracellular purine metabolism as well, because of its capacity as a regulator of the concentration of extracellular adenosine that is able to activate adenosine receptors (ARs). In recent years, other important roles have been described for ADA. One of these, with special relevance in immunology, is the capacity of ADA to act as a costimulator, promoting T-cell proliferation and differentiation mainly by interacting with the differentiation cluster CD26. Another role is the ability of ADA to act as an allosteric modulator of ARs. These receptors have very general physiological implications, particularly in the neurological system where they play an important role. Thus, ADA, being a single chain protein, performs more than one function, consistent with the definition of a moonlighting protein. Although ADA has never been associated with moonlighting proteins, here we consider ADA as an example of this family of multifunctional proteins. In this review, we discuss the different roles of ADA and their pathological implications. We propose a mechanism by which some of their moonlighting functions can be coordinated. We also suggest that drugs modulating ADA properties may act as modulators of the moonlighting functions of ADA, giving them additional potential medical interest.

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Section: Ada As An Enzyme Structural and Catalytic Characteristicsmentioning

confidence: 99%