Bovine serum albumin-templated MnO2 nanoparticles are peroxidase mimics for glucose determination by luminol chemiluminescence

Si, Xiaoxue; Song, Xiaohong; Xu, Kun; Zhao, Chao; Wang, Juan; Liu, Yu‐Shen; He, Shiyu; Jin, Minghua; Li, Hui

doi:10.1016/j.microc.2019.104050

Cited by 18 publications

(7 citation statements)

References 28 publications

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“…Mn-O vibration is attributed to the presence of small peak at 890 and 782 cm −1 suggesting the α-MnO 2 conformation of NPs [36]. The additional peaks at 1650 and 1548 cm −1 is contributed from C=O vibrations which corresponds to BSA-MnO 2 NPs synthesized by Si et al [37]. The FTIR spectra thus further confirm the formation of stabilized BSA-MnO 2 NPs.…”

Section: Characterization Of Synthesized Bsa-mno 2 Nanoparticlesmentioning

confidence: 53%

In vitro antioxidant activity of synthesized BSA conjugated manganese dioxide nanoparticles

2020

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Nanozymes represent the class of nanomaterials exhibiting enzyme mimicking properties. Biocompatible nanozymes with the ability to imitate antioxidant enzymes hold tremendous possibility for development of therapeutics in various disorders related to stress. In the present work we synthesized BSA templated MnO 2 nanoparticles. Synthesized nanoparticles was found to mimic antioxidant enzymes viz, superoxide dismutase, catalase and peroxidase. Additionally, it also showed oxidase mimicking activity. Nanoparticles were assessed for cytotoxicity by MTT assay in mammalian cells by treating human embryonic kidney cells (HEK 293) at varying doses and time period. Morphological variations induced after treatment of HEK 293 with nanoparticles was analyzed by transmission electron microscopy. Nanoparticles at 50 µg/mL concentration were found to significantly increase viability of HEK 293 cells compared to control due to their inherent antioxidant property. But at higher doses nanoparticles induced toxicity. This may be due to the intrinsic oxidase mimicking activity of the nanoparticles which suggest that at higher doses nanoparticle has cross over role as pro-oxidant. Additionally, there was no visible morphological alteration in TEM micrograph of treated cells. The results indicated that the BSA template MnO 2 particles act as pro-oxidant at higher doses due to its oxidase mimicking property however, at an optimum dose it functions as therapeutic under stress related conditions due to its anti-oxidant property.

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Section: Characterization Of Synthesized Bsa-mno 2 Nanoparticlesmentioning

confidence: 53%

In vitro antioxidant activity of synthesized BSA conjugated manganese dioxide nanoparticles

2020

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“…Luminol is the luminescence agent compared with most previously published methods. [7,10,[32][33][34] This result indicated that Fe-PorCOF was a good catalyst for the luminol reaction.…”

Section: Influence Of Experimental Conditions On the Reactionmentioning

confidence: 78%

A novel peroxidase/oxidase mimetic Fe‐porphyrin covalent organic framework enhanced the luminol chemiluminescence reaction and its application in glucose sensing

Zhao

et al. 2020

Luminescence

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A Fe‐porphyrin covalent organic framework (Fe‐PorCOF) was prepared through a postmodification strategy and characterized using different techniques. Fe‐PorCOF exhibits an inherent peroxidase/oxidase mimetic catalytic activity and sharply accelerates chemiluminescence (CL) reactions between luminol and hydrogen peroxide (H2O2) or dissolved oxygen (O2) under alkaline conditions. The catalytic role was attributed to a significant increase in production of reactive oxygen species. Using the imminent peroxidase mimetic catalytic activity of Fe‐PorCOF, a new CL method was developed for determination of H2O2 over a linear range from 0.01 to 10.0 μmol·L−1 and with a limit of detection of 5.3 nmol·L−1. The combination of the peroxidase mimetic catalytic activity of Fe‐PorCOF with the catalytic activity of glucose oxidase on glucose oxidation presents a sensitive CL method for glucose assay. The linear range and the detection limit for glucose were 0.05–8.0 μmol·L−1 and 4.0 nmol·L−1, respectively. The practicability of this method was assessed by determination of glucose in human sera. As a peroxidase/oxidase mimetic, Fe‐PorCOF is easy to prepare and exhibits good catalytic efficiency in the luminol reaction. We believe that this strategy will promote the development of a CL field with functional COFs as a catalyst.

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“…The chemiluminescence method was used for rapid determination of glucose by using bovine serum albumin (BSA)-templated MnO 2 NPs . The chemiluminescence of luminol was caused by generating H 2 O 2 as an oxidizing agent by glucose and MnO 2 NPs as the catalyst.…”

Section: Type Of Biosensors Based On Transducersmentioning

confidence: 99%

“…It has been reported that MnO 2 nanomaterials possess excellent catalytic activity for the determination of GSH and glucose by the colorimetric immunoassay. They can be used to catalyze luminol chemiluminescence under alkaline conditions . It was reported in the past few years that MnO 2 nanomaterials exhibit good oxidase-like activity by oxidizing 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) and TMB molecules into their oxidized forms in the absence of H 2 O 2 .…”

Section: Mno2 Nanomaterials As Mimic Enzymementioning

confidence: 99%

“…The chemiluminescence method was used for rapid determination of glucose by using bovine serum albumin (BSA)-templated MnO 2 NPs. 88 The chemiluminescence of luminol was caused by generating H 2 O 2 as an oxidizing agent by glucose and MnO 2 NPs as the catalyst. The MnO 2 NPs showed extraordinary catalytic activity by having properties such as great dispersion, small size, and high surface area.…”

Section: Electrochemical Biosensor By Using Conventionalmentioning

confidence: 99%

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Biosensors Based on MnO₂ Nanostructures: A Review

Sohal

Maity

Shetti

et al. 2021

ACS Appl. Nano Mater.

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In recent years, a variety of biosensors based on manganese dioxide (MnO 2 ) nanostructures were used for the detection of different biomolecules. Structural versatility and different oxidation states of Mn present in MnO 2 nanostructures significantly enhance their biosensing applications. In this review, we have mainly focused on different morphologies of MnO 2 nanostructures and their role in different types of biosensors. We are elaborately discussing the electrochemical and optical-based biosensors on different morphologies of MnO 2 and their sensing techniques with proposed mechanisms. In electrochemical biosensors, various electrodes are available but there is a need for cheap, flexible, portable, and nontoxic electrodes. Therefore, we have discussed the fabrication of MnO 2 nanostructures on the surfaces of different types of electrodes and their real-time applications. MnO 2 nanomaterials are used in various optical biosensors due to their excellent light absorption properties, and they act as strong quenching agents. MnO 2 nanomaterials are termed "alternative natural enzymes" due to their significant enzyme-like activity. The study of various morphology-dependent sensing properties of MnO 2 nanostructures provides an insight into the development of many electrochemical and optical biosensors. Recently, MnO 2 based biosensors have earned great popularity as an alternative method for fast and easy detection of many biomolecules with very low detection limits.

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Bovine serum albumin-templated MnO2 nanoparticles are peroxidase mimics for glucose determination by luminol chemiluminescence

Cited by 18 publications

References 28 publications

In vitro antioxidant activity of synthesized BSA conjugated manganese dioxide nanoparticles

In vitro antioxidant activity of synthesized BSA conjugated manganese dioxide nanoparticles

A novel peroxidase/oxidase mimetic Fe‐porphyrin covalent organic framework enhanced the luminol chemiluminescence reaction and its application in glucose sensing

Biosensors Based on MnO₂ Nanostructures: A Review

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Bovine serum albumin-templated MnO2 nanoparticles are peroxidase mimics for glucose determination by luminol chemiluminescence

Cited by 18 publications

References 28 publications

In vitro antioxidant activity of synthesized BSA conjugated manganese dioxide nanoparticles

In vitro antioxidant activity of synthesized BSA conjugated manganese dioxide nanoparticles

A novel peroxidase/oxidase mimetic Fe‐porphyrin covalent organic framework enhanced the luminol chemiluminescence reaction and its application in glucose sensing

Biosensors Based on MnO2 Nanostructures: A Review

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Biosensors Based on MnO₂ Nanostructures: A Review