Bovine serum albumin-gold nanoclusters protein corona stabilized polystyrene nanoparticles as dual-color fluorescent nanoprobes for breast cancer detection

Wu, Tingting; Chen, Keying; Lai, Wenjie; Zhou, Huicong; Wen, Xin; Chan, Hon Fai; Li, Mingqiang; Wang, Haixia; Yu, Tao

doi:10.1016/j.bios.2022.114575

Cited by 12 publications

(3 citation statements)

References 30 publications

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“…Even so, the sensors developed so far have mostly one or two signal modes and rarely three. Specifically, sensors are used to detect breast cancer-related biomarkers, with their output signals being fluorescence, , colorimetric, electrochemical, photoelectrochemical, microwave, and so on. Undoubtedly, multimode sensors are necessary.…”

Section: Introductionmentioning

confidence: 99%

Cu-MOF/Aptamer/Magnetic Bead Sensor for Detection of Breast Cancer Biomarkers HER2 and ER

Liu,

Zhang,

et al. 2024

ACS Appl. Nano Mater.

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Cancer markers are used for early cancer detection and follow-up monitoring so that patients receive the most appropriate treatment to alleviate suffering and prolong life. Herein, a metal− organic framework (MOF) combined with magnetic beads (MB) is proposed as a nanosensor for detecting breast cancer biomarkers based on magnetic enrichment and separation. With the porous structure of Cu-MOF, a large number of aptamers are adsorbed inside the pore to specifically capture the antigen. Then, Cu-MOF@antigen is enriched and separated by MB modified with the corresponding antibody. Supernatant Cu-MOF and Cu-MOF@antigen@MB are used as colorimetric, fluorescent, and electrochemical sensing elements for the quantitative detection of HER2 and ER. The detection ranges for HER2 and ER are 4.5 fg/mL−20 ng/mL and 10 fg/mL−20 ng/mL, respectively. It also demonstrated good performance in the detection of human serum samples, with recoveries ranging from 89.00 to 107.57%. This nanosensor has promising selectivity, stability, and practical applicability, providing the possibility for its in-depth development.

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Section: Introductionmentioning

confidence: 99%

Cu-MOF/Aptamer/Magnetic Bead Sensor for Detection of Breast Cancer Biomarkers HER2 and ER

Liu,

Zhang,

et al. 2024

ACS Appl. Nano Mater.

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“…Unlike nanorods, GNCs consist of a few to hundreds of gold atoms with discrete electronic structures. The ultra-small size and well-defined molecular structure lead to molecule-like properties, such as enzyme-like activities [ 26 , 27 ], fluorescence [ 22 , 28 ], high renal clearance, and long blood circulation [ 29 ]. These unique properties, attributed to the quantum confinement effect [ 30 , 31 , 32 ], make GNCs promising photosensitizers for hyperthermia-based therapy.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanomaterial System That Enables Dual Photothermal and Chemotherapy for Breast Cancer

Wang,

Shrestha,

Brey

et al. 2023

Pharmaceutics

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This study involves the fabrication and characterization of a multifunctional therapeutic nanocomposite system, as well as an assessment of its in vitro efficacy for breast cancer treatment. The nanocomposite system combines gold nanorods (GNRs) and gold nanoclusters (GNCs) to enable a combination of photothermal therapy and doxorubicin-based chemotherapy. GNRs of various sizes but exhibiting similar absorbance spectra were synthesized and screened for photothermal efficiency. GNRs exhibiting the highest photothermal efficiency were selected for further experiments. GNCs were synthesized in bovine serum albumin (BSA) and integrated into citrate-capped GNRs using layer-by-layer assembly. Glutaraldehyde crosslinking with the lysine residues in BSA was employed to immobilize the GNCs onto the GNRs, forming a stable “soft gel-like” structure. This structure provided binding sites for doxorubicin through electrostatic interactions and enhanced the overall structural stability of the nanocomposite. Additionally, the presence of GNCs allowed the nanocomposite system to emit robust fluorescence in the range of ~520 nm to 700 nm for self-detection. Hyaluronic acid was functionalized on the exterior surface of the nanocomposite as a targeting moiety for CD44 to improve the cellular internalization and specificity for breast cancer cells. The developed nanocomposite system demonstrated good stability in vitro and exhibited a pH- and near-infrared-responsive drug release behavior. In vitro studies showed the efficient internalization of the nanocomposite system and reduced cellular viability following NIR irradiation in MDA-MB-231 breast cancer cells. Together, these results highlight the potential of this nanocomposite system for targeted breast cancer therapy.

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“…Gold nanoparticles that form conjugates with polymer molecules are widely used in the creation and modification of various chemical sensors based on the effects of surface-enhanced Raman scattering, surface plasmon resonance, and F?rster energy transfer between macrochain-bound nanoobjects [1][2][3][4][5][6]. In addition, such hybrid nanosystems are used for drug delivery, as nanoprobes in biomedicine, and also in other elements of nanoelectronic devices [7][8][9][10][11][12][13]. In this case, not only spherical gold nanoparticles can be used, but also ellipsoidal nanoparticles, for example, in the form of elongated and oblate nanospheroids, to obtain nanosystems with adjustable plasmon characteristics [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Molecular dynamics simulation of the rearrangement of polyampholyte conformations on the surface of a charged oblate metal nanospheroid in a microwave electric field

Kruchinin

2023

Nanosystems: Phys. Chem. Math.

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Using molecular dynamics, the rearrangement of the conformational structure of polyampholytes on the surface of a gold oblate nanospheroid in an alternating electric field was studied depending on the value of its total charge. On the surface of the nanospheroid, at its high total charge and at small amplitude of the alternating electric field strength vector, polyampholyte loops stretched over the entire surface of the nanospheroid. With an increase in the amplitude of the electric field in the equatorial region of the nanospheroid, an annular polyampholytic fringe was formed, ordered by the types of links depending on the distance to the polarization axis of the nanoparticle. In the case of high simulation temperature, the shape of the annular fringe changed twice over a period: in one case, ordering according to the types of links along the polarization axis of the nanospheroid, and in the other case, perpendicular to it. KEYWORDS polyampholyte, oblate metallic nanospheroid, conformational changes, adsorption, molecular dynamics ACKNOWLEDGEMENTS This work was supported by the Ministry of Science and Higher Education of the Russian Federation within the framework of project no. FSGU-2023-0003. FOR CITATION Kruchinin N.Yu. Molecular dynamics simulation of the rearrangement of polyampholyte conformations on the surface of a charged oblate metal nanospheroid in a microwave electric field. Nanosystems:

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Bovine serum albumin-gold nanoclusters protein corona stabilized polystyrene nanoparticles as dual-color fluorescent nanoprobes for breast cancer detection

Cited by 12 publications

References 30 publications

Cu-MOF/Aptamer/Magnetic Bead Sensor for Detection of Breast Cancer Biomarkers HER2 and ER

Cu-MOF/Aptamer/Magnetic Bead Sensor for Detection of Breast Cancer Biomarkers HER2 and ER

Gold Nanomaterial System That Enables Dual Photothermal and Chemotherapy for Breast Cancer

Molecular dynamics simulation of the rearrangement of polyampholyte conformations on the surface of a charged oblate metal nanospheroid in a microwave electric field

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