Bovine serum albumin detection and quantitation based on capacitance measurements of liquid crystals

Lin, Chi Hao; Lee, Mon-Juan; Lee, Wei

doi:10.1063/1.4962169

Cited by 26 publications

(26 citation statements)

References 22 publications

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“…Although the data are somewhat scattered, the coefficient of determination r 2 for the linear fit is still greater than 0.92 in each case. When BSA was immobilized on a DMOAP-coated glass surface, we observed that the detection limit of BPLC-based protein assay, 10 −9 g/ml (15 pM) BSA, was similar to that based on voltage-dependent capacitance measurement of HDN, but was three orders of magnitude higher than that of the CLC-based protein assay (10 −12 g/ml), as described in our previous work [12,17]. Other studies on LC-based BSA detection demonstrated that biosensing with 5CB on a glass surface functionalized with gold nanoparticles resulted in a detection limit of 0.5 μg/ml (7.5 nM) BSA based on optical texture observation [26].…”

Section: Resultssupporting

confidence: 71%

“…By exploiting the Bragg reflection of CLC and transmission spectroscopy, the concentration of BSA can be correlated with the minimum transmittance or bandwidth of incident light [12]. Through the measurement of capacitance properties of LCs under an externally applied electric field, we observed that BSA quantitation can also be achieved by measuring the voltage-dependent capacitance of nematic LC cells containing immobilized BSA [17]. Nevertheless, quantitative biosensing technology based on LCs remains at an early stage of development.…”

Section: Introductionmentioning

confidence: 99%

“…Traditional protein assays are easy to perform with an absorption spectrometer, but the concentration range of linearity is usually limited to an order of magnitude. In LCbased protein assays, linearity may be expanded to three to four orders of magnitude according to our previous reports [12,17]. By controlling the temperature within the ranges of the BP and smectic A (SmA) phase existence, we analyzed the transmission spectra of BPLC interfaced with BSA at various concentrations along with complementary optical texture observations.…”

Section: Introductionmentioning

confidence: 99%

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Label-free protein sensing by employing blue phase liquid crystal

Lee¹,

Chang²,

Lee³

2017

Biomed. Opt. Express

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Blue phases (BPs) are mesophases existing between the isotropic and chiral nematic phases of liquid crystals (LCs). In recent years, blue phase LCs (BPLCs) have been extensively studied in the field of LC science and display technology. However, the application of BPLCs in biosensing has not been explored. In this study, a BPLC-based biosensing technology was developed for the detection and quantitation of bovine serum albumin (BSA). The sensing platform was constructed by assembling an empty cell with two glass slides coated with homeotropic alignment layers and with immobilized BSA atop. The LC cells were heated to isotropic phase and then allowed to cool down to and maintained at distinct BP temperatures for spectral measurements and texture observations. At BSA concentrations below 10 −6 g/ml, we observed that the Bragg reflection wavelength blueshifted with increasing concentration of BSA, suggesting that the BP is a potentially sensitive medium in the detection and quantitation of biomolecules. By using the BPLC at 37 °C and the same polymorphic material in the smectic A phase at 20 °C, two linear correlations were established for logarithmic BSA concentrations ranging from 10 −9 to 10 −6 g/ml and from 10

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Section: Resultssupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Label-free protein sensing by employing blue phase liquid crystal

Lee¹,

Chang²,

Lee³

2017

Biomed. Opt. Express

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“…All these advantages lend LC-based biosensors great potential for the next generation of high-sensitivity, low-cost and label-free bioassays. Up to now, various investigations of LC-based biosensors have been reported [427][428][429][430][431][432]. Very recently, Lee et al reported a label-free protein (bovine serum albumin, BSA) quantitative detection by a dual-frequency LC (DFLC)-based biosensor [433].…”

Section: Biosensorsmentioning

confidence: 99%

Perspectives in Liquid-Crystal-Aided Nanotechnology and Nanoscience

2019

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The research field of liquid crystals and their applications is recently changing from being largely focused on display applications and optical shutter elements in various fields, to quite novel and diverse applications in the area of nanotechnology and nanoscience. Functional nanoparticles have recently been used to a significant extent to modify the physical properties of liquid crystals by the addition of ferroelectric and magnetic particles of different shapes, such as arbitrary and spherical, rods, wires and discs. Also, particles influencing optical properties are increasingly popular, such as quantum dots, plasmonic, semiconductors and metamaterials. The self-organization of liquid crystals is exploited to order templates and orient nanoparticles. Similarly, nanoparticles such as rods, nanotubes and graphene oxide are shown to form lyotropic liquid crystal phases in the presence of isotropic host solvents. These effects lead to a wealth of novel applications, many of which will be reviewed in this publication. the observation of chirality from achiral molecules, resulting from sterically induced packing of the bent-core mesogens [10], such as ferroelectricity or the formation of helical superstructures in the B7 phase [14]. Thermotropic LCs are commonly constituted by single organic entities or mixtures thereof, which exhibit various mesophases at different temperatures or pressures [15], illustrated in Figure 1b. As the temperature rises, a typical thermotropic LC passes through higher ordered phases, also called soft crystals, the hexatic smectic phases with positional order as well as bond orientational order, through the fluid smectic phases (SmC and SmA), which exhibit both positional and orientational order, and finally to the nematic phase (N) with purely orientational order, into the isotropic phase. The number of different phases observed depends on the chemical composition, symmetry and order of the LC molecules. About 25 different thermotropic phases are known to date, and they are still increasing in number. Appl. Sci. 2019, 9, x FOR PEER REVIEW 2 of 47 unique effects of the observation of chirality from achiral molecules, resulting from sterically induced packing of the bent-core mesogens [10], such as ferroelectricity or the formation of helical superstructures in the B7 phase [14]. Thermotropic LCs are commonly constituted by single organic entities or mixtures thereof, which exhibit various mesophases at different temperatures or pressures [15], illustrated in Figure 1b. As the temperature rises, a typical thermotropic LC passes through higher ordered phases, also called soft crystals, the hexatic smectic phases with positional order as well as bond orientational order, through the fluid smectic phases (SmC and SmA), which exhibit both positional and orientational order, and finally to the nematic phase (N) with purely orientational order, into the isotropic phase. The number of different phases observed depends on the chemical composition, symmetry and order of the LC molecules. About...

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“…However, some innovative applications of the capacitance, electrooptical, and dielectric measurements of LCs have emerged. The Lee group [58] reported a LC-based capacitive biosensor, whose LC cell can be considered a parallel-plate capacitor. LCs were homeotropically or vertically aligned by DMOAP firstly.…”

Section: Other Sensing Techniques Based On Lcmentioning

confidence: 99%

Application and Technique of Liquid Crystal-Based Biosensors

Luan

Luo

2020

Micromachines

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Liquid crystal biosensors are based on changes in the orientation of liquid crystal molecules induced by specific bonding events of biomolecules. These biosensors are expected to serve as a promising system to detect biomolecules, biomolecular activity, and even small chemical molecules because they are inexpensive, sensitive, simple, effective, and portable. Herein, we introduce the principle and fabrication of liquid crystal biosensors and review the research progress in signal-amplified technology for liquid crystal sensing and its application in the detection of viruses, bacteria, proteins, nucleic acids, and small chemical molecules. In addition, the current theoretical and practical issues related to liquid crystal biosensors were investigated.

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Bovine serum albumin detection and quantitation based on capacitance measurements of liquid crystals

Cited by 26 publications

References 22 publications

Label-free protein sensing by employing blue phase liquid crystal

Label-free protein sensing by employing blue phase liquid crystal

Perspectives in Liquid-Crystal-Aided Nanotechnology and Nanoscience

Application and Technique of Liquid Crystal-Based Biosensors

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