Bovine Rotavirus in Turkeys with Enteritis

Asano, Karen Miyuki; Gregori, Fábio; Souza, Samilly Evangelista; Rotava, D; Oliveira, R. N.; Villarreal, Laura; Richtzenhain, Leonardo José; Brandão, Paulo Eduardo

doi:10.1637/9765-041911-resnote.1

Cited by 20 publications

(27 citation statements)

References 26 publications

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“…Recently, bovine-origin rotavirus A was also found in turkeys in Brazil (2). In studies of Otto et al (20), the detection rate of rotavirus groups A and D was different and depended on the applied methods.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characteristics of rotaviruses from Polish turkey flocks between 2008 and 2011

Domańska-Blicharz

Jacukowicz

Minta

2013

Bulletin of the Veterinary Institute in Pulawy

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Between 2008 and 2011, commercial turkey flocks in Poland were examined for the presence of rotaviruses. Ten faecal swabs from each of 207 turkey flocks (turkeys aged one to 19 weeks) were collected in different regions of the country and tested using a PCR assay that targeted the NSP4 gene. The prevalence of rotavirus was 20.3% in the flocks tested. Phylogenetic analysis revealed a clear division into groups dependent on geographical origin of the analysed viruses. All Polish rotaviruses belonged to the European group. However, they were found to be genetically variable based on the sequence analysis. The most frequently identified rotaviruses belonged to RV-1 subgroup and two of them formed a distinct subgroup of RV-2. Rotaviruses were detected in healthy and enteric turkeys. The observed amino acid changes probably did not affect the group affiliation, nor the pathogenecity of the studied rotavirus strains.

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Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characteristics of rotaviruses from Polish turkey flocks between 2008 and 2011

Domańska-Blicharz

Jacukowicz

Minta

2013

Bulletin of the Veterinary Institute in Pulawy

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“…Reassortment is known to occur within mammalian and avian RVA (Ward et al, 1990;Schumann et al, 2009). However, although interspecies transmission of mammalian RVA to birds and of avian RVA to mammals can occur (Brussow et al, 1992a(Brussow et al, , 1992bMori et al, 2001;Wani et al, 2003;Asano et al, 2011), mammalian and avian RVA are believed to have diverged along with their hosts and to have evolved independently without reassortment (Ito et al, 2001;Trojnar et al, 2009). Reassortment may be suppressed not only by the incompatibility of packaging signals but also by incompatibility in the functions of proteins encoded by genomic segments (Hutchinson et al, 2010;McDonald and Patton, 2011).…”

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confidence: 99%

A possible packaging signal in the rotavirus genome

Suzuki

2014

Genes Genet. Syst.

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Group A rotavirus (RVA), an etiological agent of gastroenteritis in young mammals and birds, possesses a genome of 11 double-stranded RNA segments. Although it is believed that the RVA virion contains one copy of each genomic segment and that the positive-strand RNA (+RNA) is incorporated into the core shell, the packaging mechanisms of RVA are not well understood. Here, packaging signals of RVA were searched for by analyzing genomic sequences of mammalian and avian RVA, which are considered to have evolved independently without reassortment. Assuming that packaging is mediated by direct interaction between +RNA segments via base-pairing, co-evolving complementary nucleotide sites were identified within and between genomic segments. There were two pairs of co-evolving complementary sites within the segment encoding VP7 (the VP7 segment) and one pair between the NSP2 and NSP3 segments. In the VP7 segment, the co-evolving complementary sites appeared to form stem structures in both mammalian and avian RVA, supporting their functionality. In contrast, coevolving complementary sites between the NSP2 and NSP3 segments tended to be free from base-pairings and constituted loop structures, at least in avian RVA, suggesting that they are involved in a specific interaction between these segments as a packaging signal.

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“…In Brazil, there have been reports on avian rotavirus occurrence since 1989, with frequency ranging from 8.5% (32/378 samples) in broilers (Alfieri et al 1989) to 45.3% (58/128 samples) from intestinal contents of layers and broilers by PAGE (polyacrylamide gel electrophoresis) (Villarreal et al 2006). Most of the avian rotaviruses detected in Brazil belong to RVA , Asano et al 2011. Nevertheless, RVD and RVF have been recently described in the country (Bezerra et al 2012, Bezerra et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Monitoring and molecular characterization of group Drotavirus in Brazilian poultry farms

et al. 2015

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RESUMO.-[Monitoramento e caracterização molecular de rotavírus do grupo D em criações comerciais brasileiras.] Rotavírus são agentes etiológicos de diarreia tanto em humanos como em várias espécies animais. Dados sobre rotavírus do grupo D (RVD) em aves são escassos, especialmente no Brasil. Nós detectamos RVD em 4 pools de conteúdo intestinal de frango de corte, poedeiras e matrizes de um total de 111 pools originários de 8 estados brasileiros, representando uma ocorrência de 3,6% a partir de uma RT-PCR específica para RVD, tendo como alvo o gene VP6. A árvore filogenética confirmou que as amostras brasileiras pertencem ao grupo D e três das sequências obtidas foram idênticas em termos de aminoácidos enquanto uma apresentou 99,5% de identidade com as demais. As sequências aqui definidas são semelhantes a outras sequências previamente definidas no Brasil, confirmando a natureza conservada da proteína VP6. INTRODUCTIONRotaviruses are members of the Reoviridae family, and they are a major cause of acute gastroenteritis in young children and several mammalian and avian species (Brüssow et al. 1992, Estes & Kapikian 2007. They are non-enveloped icosahedral particles and its genome comprises 11 segments of double-stranded RNA, which encodes six structural proteins (VP1 to VP4, VP6 and VP7) and six nonstructural proteins (NSP1 to NSP6). Based on antibody reactivity or genetic sequencing of structural protein VP6 of the internal capsid, rotaviruses have been classified into the groups A to G ( Brazil. E-mail: laila_andreia@hotmail.com Rotaviruses are etiological agents of diarrhea both in humans and in several animal species. Data on avian Group D rotaviruses (RVD) are scarce, especially in Brazil. We detected RVD in 4 pools of intestinal contents of broilers, layer and broiler breeders out of a total of 111 pools from 8 Brazilian states, representing an occurrence of 3.6%, by a specific RVD RT-PCR targeting the VP6 gene. Phylogenetic tree confirmed that the Brazilian strains belong to group D and 3 of the sequences were identical in terms of amino acid whereas one showed 99.5% identity with the others. The sequences described in this study are similar to other sequences previously detected in Brazil, confirming the conserved nature of the VP6 protein.

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Bovine Rotavirus in Turkeys with Enteritis

Cited by 20 publications

References 26 publications

Prevalence and molecular characteristics of rotaviruses from Polish turkey flocks between 2008 and 2011

Prevalence and molecular characteristics of rotaviruses from Polish turkey flocks between 2008 and 2011

A possible packaging signal in the rotavirus genome

Monitoring and molecular characterization of group Drotavirus in Brazilian poultry farms

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