Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily

Savini, Federica; Gallina, Laura; Prosperi, Alice; Puleio, Roberto; Llombart‐Bosch, Antonio; Marco, Patrizia Di; Tumino, Serena; Moreno, Ana; Lelli, Davide; Guercio, Annalisa; Scagliarini, Alessandra

doi:10.3390/pathogens9060429

Cited by 14 publications

(26 citation statements)

References 44 publications

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“…δPVs are the only BPVs known to infect mesenchymal tissues and to show cross-species transmission and infection 1 . δPVs have been detected in cutaneous wart lesions from ovines 10 , 11 . Vertical transmission of δPVs in sheep, resulting in oral fibropapillomatosis and epidermal hyperplasia of the lip in newborn lambs, has also been documented 12 .…”

Section: Introductionmentioning

confidence: 99%

Detection and quantification of bovine papillomavirus DNA by digital droplet PCR in sheep blood

Roperto

Cutarelli

Corrado

et al. 2021

Sci Rep

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Highly pathogenic bovine papillomaviruses (BPVs) were detected and quantified for the first time using digital droplet polymerase chain reaction (ddPCR) by liquid biopsy in 103 clinically healthy sheep. Overall, ddPCR detected BPVs in 68 blood samples (66%). BPV infection by a single genotype was revealed in 61.8% of the blood samples, and BPV coinfection by double, triple or quadruple genotypes was observed in 38.2% of liquid biopsies. The BPV-2 genotype was most frequently seen in sheep, whereas BPV-1 was the least common. Furthermore, ddPCR was very useful for detection and quantification; the BPV-14 genotype was observed for the first time in ovine species, displaying the highest prevalence in some geographical areas (Apulia). In 42 of the positive samples (61.8%), a single BPV infection was observed, 26 of which were caused by BPV-2 (61.9%) and 7 by BPV-13 (16.7%). BPV-14 was responsible for 7 single infections (16.7%) and BPV-1 for 2 single infections (4.7%). Multiple BPV coinfections were observed in the remaining 26 positive samples (38.2%), with dual BPV-2/BPV-13 infection being the most prevalent (84.6%). BPV infection by triple and quadruple genotypes was also observed in 11.5% and 3.8% of cases, respectively. The present study showed that ddPCR, a biotechnological refinement of conventional PCR, is by far the most sensitive and accurate assay for BPV detection compared to conventional qPCR. Therefore, ddPCR displayed an essential diagnostic and epidemiological value very useful for the identification of otherwise undetectable BPV genotypes as well as their geographical distributions and suggesting that animal husbandry practices contribute to cross-species transmission of BPVs.

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Section: Introductionmentioning

confidence: 99%

Detection and quantification of bovine papillomavirus DNA by digital droplet PCR in sheep blood

Roperto

Cutarelli

Corrado

et al. 2021

Sci Rep

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“…It is worth noting that outbreaks of CEH have also been reported in sheep [27,28]. Furthermore, bovine δPV infection resulting in clinical disease are known to occur in sheep [8][9][10]. Therefore, the detection of bovine δPV DNA in the blood of sheep means that sheep can be infected by these PVs, which may make δPVs an additional, potential cause of ovine disease.…”

Section: Discussionmentioning

confidence: 99%

“…δPVs are the only BPVs known to infect mesenchymal tissues and to show cross-species transmission and infection [1]. δPVs have been detected in cutaneous wart lesions from ovines [8,9]. Vertical transmission of δPVs in sheep, resulting in oral bropapillomatosis and epidermal hyperplasia of the lip in newborn lambs, has also been documented [10].…”

Section: Introductionmentioning

confidence: 99%

Detection and Quantification of Bovine Papillomavirus DNA by Digital Droplet PCR in Sheep Blood

Roperto

Cutarelli

Corrado

et al. 2021

Preprint

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Highly pathogenic bovine Delta papillomaviruses (δPVs) were detected and quantified for the first time using digital droplet polymerase chain reaction (ddPCR) by liquid biopsy in 103 clinically healthy sheep. Overall, ddPCR detected bovine δPVs in 68 blood samples (66%). Bovine papillomavirus (BPV) infection by a single genotype was revealed in 59% of the blood samples, and BPV coinfection by double, triple or quadruple genotypes was observed in 41% of liquid biopsies. The BPV-2 genotype was most frequently seen in sheep, whereas BPV-1 was the least common. Furthermore, ddPCR was very useful for detection and quantification; the BPV-14 genotype was observed for the first time in ovine species, displaying the highest prevalence in some geographical areas (Apulia). In 42 of the positive samples (61.8%), a single BPV infection was observed, 26 of which were caused by BPV-2 (61.9%) and 7 by BPV-13 (16.7%). BPV-14 was responsible for 7 single infections (16.7%) and BPV-1 for 2 single infections (4.7%). Multiple BPV coinfections were observed in the remaining 26 positive samples (38.2%), with dual BPV-2/BPV-13 infection being the most prevalent (84.6%). BPV infection by triple and quadruple genotypes was also observed in 11.5% and 3.8% of cases, respectively. The present study showed that ddPCR, a biotechnological refinement of conventional PCR, is by far the most sensitive and accurate assay for BPV detection. Therefore, ddPCR displayed diagnostic and epidemiological value resulting in the identification of otherwise undetectable BPV genotypes as well as their geographical distributions and suggesting that animal husbandry practices contribute to cross-species transmission of BPVs..

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“…Bovine papillomavirus (BPV) is an oncogenic virus belonging to the Papillomaviridae family, which infects the epithelium and mucosa of many animals including cattle. Although papillomaviruses are usually species-specific, BPV has been observed in other animals such as giraffes, buffalo, sheep and horses [1][2][3][4][5][6][7]. BPVs are classified into five genera and 28 types have been identified so far.…”

Section: Introductionmentioning

confidence: 99%

High Genotypic Diversity, Putative New Types and Intra-Genotype Variants of Bovine Papillomavirus in Northeast Brazil

et al. 2020

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Bovine papillomavirus (BPV) can cause damage to the epithelial and mucosal tissue and currently presents 28 known types. Not all BPV types are associated with the development of cancer in cattle. Studies have shown that variants of human papillomavirus types can present different pathogenic profiles. However, despite the similarity, it is not yet known whether variants of BPV types can also present varying degrees of pathogenicity. Thus, the aim of this study was to evaluate the genetic variability of BPV types and variants isolated in Northeastern Brazil. Samples were obtained from animals with papillomatous lesions. BPV DNA was detected by the amplification of the L1 gene and genotyping was performed by sequencing. Mutations were analyzed in a phylogenetic, structural and functional context. In total, 52 positive samples were obtained and 11 different BPV types were identified in the samples. Ten putative new BPV types were also identified. In addition, several non-synonymous mutations were identified and predicted to alter protein stability, having an impact on immune evasion. The study demonstrated a high genetic diversity of BPV in the region with a large number of mutations identified, serving as a basis for more efficient control measures to be adopted for bovine papillomatosis.

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Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily

Cited by 14 publications

References 44 publications

Detection and quantification of bovine papillomavirus DNA by digital droplet PCR in sheep blood

Detection and quantification of bovine papillomavirus DNA by digital droplet PCR in sheep blood

Detection and Quantification of Bovine Papillomavirus DNA by Digital Droplet PCR in Sheep Blood

High Genotypic Diversity, Putative New Types and Intra-Genotype Variants of Bovine Papillomavirus in Northeast Brazil

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