Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation

Elhmouzi-Younes, Jamila; Storset, Anne K.; Boysen, Preben; Laurent, Fabrice; Drouet, Francçoise

doi:10.1051/vetres/2009037

Cited by 15 publications

(20 citation statements)

References 63 publications

(80 reference statements)

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“…However, cattle are large enough to handle and feed at a very young age and are often used in age-related and diet-influenced immunity studies [14, 16, 35–39]. Furthermore, the current study and other studies suggest that bovine lymphocytes are predictive for the influence of polyphenols on human lymphocytes [3, 8].…”

Section: Discussionmentioning

confidence: 64%

Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B

Ramstead

Schepetkin

Todd

et al. 2015

International Immunopharmacology

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Several plant extracts, including certain polyphenols, prime innate lymphocytes and enhance responses to secondary stimuli. Oenothein B, a polyphenol isolated from Epilobium angustifolium and other plant sources, enhances IFNγ production by both bovine and human NK cells and T cells, alone and in response to secondary stimulation by cytokines or tumor cells. Innate immune cell responsiveness is known to be affected by aging, but whether polyphenol responses by these cells are also impacted by aging is not known. Therefore, we examined oenothein B responsiveness in T cells from cord blood, young, and adult donors. We found that oenothein B stimulates bovine and human T cells from individuals over a broad range of ages, as measured by increased IL-2Rα and CD69 expression. However, clear differences in induction of cytokine production by T cells were seen. In T cells from human cord blood and bovine calves, oenothein B was unable to induce IFNγ production. However, oenothein B induced IFNγ production by T cells from adult humans and cattle. In addition, oenothein B induced GM-CSF production by human adult T cells, but not cord blood T cells. Within the responsive T cell population, we found that CD45RO+ memory T cells expressed more cytokines in response to oenothein B than CD45RO− T cells. In summary, our data suggest that the immunostimulation of T cells by oenothein B is influenced by age, particularly with respect to immune cytokine production.

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Section: Discussionmentioning

confidence: 64%

Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B

Ramstead

Schepetkin

Todd

et al. 2015

International Immunopharmacology

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“…Steps were taken to limit such confounders: Laboratory analyses were carried out using standardized protocols by the same person, often repeated several times. All animals were recruited from a similar age group since age has proven to be a significant variable in NK cell biology ( 54 – 56 ). Finally, no breed influence was detected in parameters measured in PBMC (Figures 2 and 3 ).…”

Section: Discussionmentioning

confidence: 99%

Natural Killer Cells in Afferent Lymph Express an Activated Phenotype and Readily Produce IFN-γ

et al. 2013

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Natural killer (NK) cells are motile cells that migrate between peripheral blood (PB), lymph nodes (LNs), and various organs. Domestic animals have frequently been used to study cellular migration, and offer unique opportunities for such studies. The aim of this study was to characterize the phenotype and cytokine producing capacity of NK cells in bovine skin-draining lymph. NKp46/NCR1+ CD3− cells constituted 2–11% of mononuclear cells in afferent lymph (AL), a majority of cells were CD16+, CD8α+, and CD2−/low, and elevated CD25 and CD44 expression indicated an activated phenotype. Interestingly, significantly fewer AL NK cells expressed the early activation marker CD69 compared to PB NK cells. A large proportion of lymph and blood NK cells produced interferon (IFN)-γ following stimulation with IL-2 and IL-12. Notably, in AL, but not blood, a similar amount of IFN-γ+ NK cells was observed when cells were stimulated with IL-12 alone. Overall, AL NK cells were more similar to LN-residing NK cells than those circulating in PB. We conclude that AL appears to be an important migration route for tissue-activated NK cells, and may represent an alternative route for NK cell traffic to LNs. These findings may have important implications in the development of adjuvant strategies that aim to target NK cells in a vaccine response.

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“…The specialized follicle associated epithelium (FAE) that overlies PPs is capable of transporting luminal antigens [ 29 ] to the underlying immune cells to promote a tolerogenic or an inflammatory response, which will be set in action in the lamina propria. Our aim was to get an insight into the early local immune response in the different sections of the small intestine and associated lymphoid tissues of lambs during the neonatal period with a particular focus on NK cells, which we have shown to be active in neonatal calves [ 30 ], and CD8 T lymphocytes, that have been shown to be important in controlling C. parvum infection in humans [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1− CD8+ cell recruitment

Olsen

Åkesson

Storset

et al. 2015

Vet Res

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Cryptosporidium parvum, a zoonotic protozoan parasite, causes important losses in neonatal ruminants. Innate immunity plays a key role in controlling the acute phase of this infection. The participation of NCR1+ Natural Killer (NK) cells in the early intestinal innate immune response to the parasite was investigated in neonatal lambs inoculated at birth. The observed increase in the lymphocyte infiltration was further studied by immunohistology and flow cytometry with focus on distribution, density, cellular phenotype related to cytotoxic function and activation status. The frequency of NCR1+ cells did not change with infection, while their absolute number slightly increased in the jejunum and the CD8+/NCR1- T cell density increased markedly. The frequency of perforin+ cells increased significantly with infection in the NCR1+ population (in both NCR1+/CD16+ and NCR1+/CD16- populations) but not in the NCR1-/CD8+ population. The proportion of NCR1+ cells co-expressing CD16+ also increased. The fraction of cells expressing IL2 receptor (CD25), higher in the NCR1+/CD8+ population than among the CD8+/NCR1- cells in jejunal Peyer’s patches, remained unchanged during infection. However, contrary to CD8+/NCR1- lymphocytes, the intensity of CD25 expressed by NCR1+ lymphocytes increased in infected lambs. Altogether, the data demonstrating that NK cells are highly activated and possess a high cytotoxic potential very early during infection, concomitant with an up-regulation of the interferon gamma gene in the gut segments, support the hypothesis that they are involved in the innate immune response against C. parvum. The early significant recruitment of CD8+/NCR1- T cells in the small intestine suggests that they could rapidly drive the establishment of the acquired immune response.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-014-0136-1) contains supplementary material, which is available to authorized users.

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Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation

Cited by 15 publications

References 63 publications

Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B

Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B

Natural Killer Cells in Afferent Lymph Express an Activated Phenotype and Readily Produce IFN-γ

The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1− CD8+ cell recruitment

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