Bovine natural resistance associated macrophage protein 1 (Nramp1) gene.

Abstract

The BcglltylLsh locus is a major gene controlling early phases of infection with intracellular parasites in mice.Natural resistance associated macrophage protein 1 (Nrampl) has been shown to be the Beg gene in mice. Analysis of a bovine cDNA homolog of murine Nrampb designated as bovine NRAMPI, predicted a 548-amino-acid protein with hydrophobic domains, an amino-terminal SH3-binding domain, and a conserved consensus transport motif. Northern blotting indicated that bovine NRAMP1 was expressed primarily in macrophages and tissues of the recticuloendothelial system. Bovine NRAlqPI was mapped to BTA 2 within syntenic loci conserved on HSA 2q and MMU I.

Identification of single nucleotide polymorphisms in the bovine solute carrier family 11 member 1 (SLC11A1) gene and their association with infection by Mycobacterium avium subspecies paratuberculosis

“…As for the putative functional effect of these alleles, it is striking that c.1067C > G is located in exon 11, which encodes for transmembrane domain 8 (TM8), the most conserved region of the 12 putative transmem-brane domains of the SLC11A1 protein (Feng et al, 1996). Indeed, SNP c.1067C > G is the only variable nucleotide that we found in the TM8 domain.…”

Identification of genetic loci controlling bacterial clearance in experimental Salmonella enteritidis infection: an unexpected role of Nramp1 (Slc11a1) in the persistence of infection in mice

“…9,14,17 Nramp1 is part of an ancient family of proteins with highly conserved members in mammals, birds, invertebrates, plants, fungi and even bacteria. [18][19][20][21][22][23][24][25][26] Nramp1 is an integral membrane phosphoglycoprotein located in the late endosome/lysosome compartment of resting macrophages and is recruited to the phagosomal membrane during phagosome maturation 27 that encodes for a divalent-cation transporter. 28 Several additional loci have been found to exert some control during the late phase of infection through their effects on the acquired immune responses.…”

Genetic Resistance toBrucella abortusin the Water Buffalo (Bubalus bubalis)

“…This observation suggests that genetic variation within the host may play a part in the resistance to brucellosis. In cattle, it is known that the resistance to brucellosis is genetically determined (15,38,40). These circumstances, and the widespread presence of genes protecting against bacterial infections in livestock (15,23,30), humans (6,25,34,43), mice (27,36), and invertebrates (26,29) prompted the search for polymorphisms conferring resistance to brucellosis in the water buffalo.…”

“…The mouse Nramp1 gene confers resistance to several unrelated intracellular pathogens, including Salmonella enterica serovar Typhimurium, Leishmania donovani, and Mycobacterium bovis BCG (37,44). The human Nramp1 gene confers resistance to Mycobacterium tuberculosis (6), and the cattle Nramp1 gene confers resistance to Brucella abortus (15). As to the mechanism by which Nramp1 confers innate resistance to intracellular pathogens, it has been proposed that the product of Nramp1 may limit microbial replication in the phagosome by subtracting critical nutrients to invading microbes (18).…”