Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis

Reinhardt, Timothy A.; Sacco, Randy E.; Nonnecke, Brian J.; Lippolis, John D.

doi:10.1016/j.jprot.2013.02.013

Cited by 162 publications

(183 citation statements)

References 52 publications

Supporting

Mentioning

163

Contrasting

Unclassified

Order By: Relevance

“…25 Glycodelin was first observed in macaque milk using immunoblotting in 1994 50 and is confirmed in this study with sequence level characterization and quantification. We identified 189 unique peptides to support its presence and 77% of the total amino acid sequence was detected using LC-MS/MS.…”

Section: Characterization Of Macaque Milk Proteinssupporting

confidence: 78%

“…Yet the most detailed proteomes for any species 15,[22][23][24][25]28,29 have identified less than 20% of the resultant protein products. While not every mRNA will yield a protein product in milk, studies have shown mRNA and protein concentrations to be clearly correlated (R 2 = 0.47-0.71) [30][31][32] indicating that only a fraction of all possible proteins in milk have been identified.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Proteomics of Human and Macaque Milk Reveals Species-Specific Nutrition during Postnatal Development

Beck¹,

Weber²,

Phinney³

et al. 2015

J. Proteome Res.

View full text Add to dashboard Cite

Milk has been well established as the optimal nutrition source for infants, yet there is still much to be understood about its molecular composition. Therefore, our objective was to develop and compare comprehensive milk proteomes for human and rhesus macaques to highlight differences in neonatal nutrition. We developed a milk proteomics technique that overcomes previous technical barriers including pervasive post-translational modifications and limited sample volume. We identified 1606 and 518 proteins in human and macaque milk, respectively. During analysis of detected protein orthologs, we identified 88 differentially abundant proteins. Of these, 93% exhibited increased abundance in human milk relative to macaque and include lactoferrin, polymeric immunoglobulin receptor, alpha-1 antichymotrypsin, vitamin D-binding protein, and haptocorrin. Furthermore, proteins more abundant in human milk compared with macaque are associated with development of the gastrointestinal tract, the immune system, and the brain. Overall, our novel proteomics method reveals the first comprehensive macaque milk proteome and 524 newly identified human milk proteins. The differentially abundant proteins observed are consistent with the perspective that human infants, compared with nonhuman primates, are born at a slightly earlier stage of somatic development and require additional support through higher quantities of specific proteins to nurture human infant maturation.

show abstract

Section: Characterization Of Macaque Milk Proteinssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Comparative Proteomics of Human and Macaque Milk Reveals Species-Specific Nutrition during Postnatal Development

Beck¹,

Weber²,

Phinney³

et al. 2015

J. Proteome Res.

View full text Add to dashboard Cite

show abstract

“…In addition, although the epithelial layer is compromised, both proteins can also be seen in association with epithelial cells by immune colocalization in the acutely mastitic, inoculated half udders. Here, however, the significant positivity of neutrophils and their massive recruitment to the infected site could also be seen, as expected from the known ability of these cells to produce and release AMPs and acutephase-response proteins, as well as from the strong chemoattractant activity exerted by these proteins at the site of infection (77). Conversely, macrophages remained negative in all samples and under all conditions examined.…”

Section: Discussionsupporting

confidence: 61%

Production and Release of Antimicrobial and Immune Defense Proteins by Mammary Epithelial Cells following Streptococcus uberis Infection of Sheep

Addis¹,

Pisanu²,

Marogna

et al. 2013

Infect Immun

View full text Add to dashboard Cite

“…In general, the different numbers of analyzed animals with regard to the included disease conditions might be a reason for controversial results from proteome analysis and ELISA in our study. In whey proteome analysis HP, cathelicidin-1, FG, CFD and several other complement factors were detected and appeared to be up-regulated during disease which is in agreement with analyses of the mastitic whey or milk by other authors [30,43,51]. For PIGR (SC) no clear tendency of regulation was observed.…”

Section: Discussionsupporting

confidence: 88%

Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approach

Zoldan¹,

Schneider²,

T³

et al. 2017

J Adv Dairy Res

View full text Add to dashboard Cite

At onset of milk production and in early lactation highly producing dairy cows are most susceptible for inflammatory diseases due to functional suppression of immune cells. Intensive supervision of the animals is essential and implementation of new technologies to on-farm routines will be the next step to provide automation and improvement of herd health monitoring programs. Objective of our study was to identify and validate immunological biomarkers in milk that indicate extra-mammary inflammatory diseases to characterize the general health status of highly-producing dairy cows.In total 89 healthy and 75 diseased animals (German Holstein cows) were included. Diseases were distinguished by either systemic (extra-mammary) occurrence or those affecting the mammary gland (mastitis) and further classified by their severity. For protein biomarker discovery we used a top-down approach to narrow down a broad range of secreted gene products of the milk cell transcriptome (microarray) and proteome to a few promising candidates which were validated using real-time PCR and ELISA. The most promising biomarker candidates were statistically evaluated. Receiver operating characteristic analysis revealed haptoglobin, secretory component, lactoferrin and vascular endothelial growth factor showing the highest discriminatory capability for diseased vs. healthy cows. Values for sensitivity at a specificity of 94% were 82% for haptoglobin, 59% for secretory component, 55% for lactoferrin and 67% for vascular endothelial growth factor. Statistical evaluation by multinomial logistic regression and k-nearest neighbor method confirmed haptoglobin as the best single-use biomarker. In combination with secretory component or lactoferrin an increase in overall sensitivity or specificity, depending on the classification method, could be achieved.The application of the validated health biomarkers in combination with an easy high-throughput detection system would offer a solution to adapt dairy herd management to changing requirements on animal welfare, farming efficiency, milk supply and food safety in modern agriculture.

show abstract

Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis

Cited by 162 publications

References 52 publications

Comparative Proteomics of Human and Macaque Milk Reveals Species-Specific Nutrition during Postnatal Development

Comparative Proteomics of Human and Macaque Milk Reveals Species-Specific Nutrition during Postnatal Development

Production and Release of Antimicrobial and Immune Defense Proteins by Mammary Epithelial Cells following Streptococcus uberis Infection of Sheep

Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approach

Contact Info

Product

Resources

About