Bovine milk derived skimmed milk powder and whey protein concentrate modulatesCitrobacter rodentiumshedding in the mouse intestinal tract

Abstract

Skimmed milk powder (SMP) and whey protein concentrate (WPC) were manufactured from fresh milk collected from cows producing high or low Immunoglobulin (Ig) A levels in their milk. In addition commercial products were purchased for use as diluent or control treatments. A murine enteric disease model (Citrobacter rodentium) was used to assess whether delivery of selected bioactive molecules (IgA, IgG, Lactoferrin (Lf)) or formulation delivery matrix (SMP, WPC) affected faecal shedding of bacteria in C. rodentium infected mice. In trial one, faecal pellets collected from mice fed SMP containing IgA (0.007–0.35 mg/mL), IgG (0.28–0.58 mg/mL) and Lf (0.03–0.1 mg/mL) contained fewer C. rodentium (cfu) compared to control mice fed water (day 8, p < 0.04, analysis of variance (ANOVA) followed by Fisher’s unprotected least significant difference (ULSD)). In trial two, WPC containing IgA (0.35–1.66 mg/mL), IgG (0.58–2.36 mg/mL) and Lf (0.02–0.45 mg/mL) did not affect C. rodentium shedding, but SMP again reduced faecal C. rodentium levels (day 12, p < 0.04, ANOVA followed by Fisher’s ULSD). No C. rodentium was detected in sham phosphate-buffered saline inoculated mice. Mice fed a commercial WPC shed significantly greater numbers of C. rodentium over 4 consecutive days (Fishers ULSD test), compared to control mice fed water. These data indicate that SMP, but not WPC, modulates faecal shedding in C. rodentium-infected mice and may impact progression of C. rodentium infection independently of selected bioactive concentration. This suggests that food matrix can impact biological effects of foods.

Mastitis Süresince Meme Epitel Hücrelerinin Fonksiyonel Durumunu Değerlendirmek Amacıyla Sığır α-kazein Proteinine Karşı Monoklonal Antikor Geliştirilmesi

“…Generally, there are four types of bovine caseins and classified as αs1-casein, αs2-casein, β-casein and κ-caseins [2] . Among these four types of caseins, α-caseins (including αs1-casein and αs2-casein) are the most abundant form of casein in bovine milk [1] . Importantly, α-caseins have been reported to be involved in a variety of physical or pathological conditions, with αs1-and αs2subtypes playing redundant but usually distinct roles [19,20] .…”

Sağlıklı ve Mastitisli Keçi Sütünde αs1-kazeini Belirleyen Monoklonal Antikor Geliştirilmesi

“…Casein expression level is an important index when evaluating the lactation function of the mammary gland. While mAbs against β-casein [10] and κ-casein [11] have been developed, no studies have reported the successful development of mAbs against αs1-casein, the major part (38%) of total caseins in milk [12] . In addition, considering that αs1-casein is the most important protein causing milk allergy [13] , mAbs with high specificity to goat αs1-casein are therefore of critical importance for people to avoid milk allergy.…”

The Reasons Behind the Salubrious of Colostrum on the Cognitive Functions: A Systematic Review

“…Several forms of pharmaceutical preparations were prepared and used for research, and many pharmaceuticals were manufactured BC. These formulations are: powder in capsule (bovine immunoglobulin concentrate) 52 ; oral bovine colostrum tablet [53] ; sublingual colostrinin tablet 54 intravaginal gel containing purified (dialyzed lyophilized) bovine colostrum (ZP-025) 55 ; spray-dried BC 56 ; and liposomal bovine colostrum. 36…”