Bovine in vitro reproduction models can contribute to the development of (female) fertility preservation strategies

Langbeen, A.; Porte, H.F.M. De; Bartholomeus, Esther; Leroy, Jo; Bols, P.E.J.

doi:10.1016/j.theriogenology.2015.04.009

Cited by 34 publications

(17 citation statements)

References 199 publications

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“…Hence, our findings provide insights on how to improve culture media and strategies of assisted reproduction in livestock. Moreover, given the relevance of the bovine model to human ovarian physiology (Langbeen et al, 2015, Polejaeva et al, 2016, our findings would likely aid studies aimed at treating human infertility.…”

Section: Discussionmentioning

confidence: 94%

Zinc supports transcription and improves meiotic competence of growing bovine oocytes

et al. 2020

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In the last years, many studies focused on the understanding of the possible role of zinc in the control of mammalian oogenesis, mainly on oocyte maturation and fertilization. However, little is known about the role of zinc at earlier stages, when the growing oocyte is actively transcribing molecules that will regulate and sustain subsequent stages of oocyte and embryonic development. In this study, we used the bovine model to gain insights into the possible involvement of zinc in oocyte development. We first mined the EmbryoGENE transcriptomic dataset, which revealed that several zinc transporters and methallothionein are impacted by physiological conditions throughout the final phase of oocyte growth and differentiation. We then observed that zinc supplementation during in vitro culture of growing oocytes is beneficial to the acquisition of meiotic competence when subsequently subjected to standard in vitro maturation. Furthermore, we tested the hypothesis that zinc supplementation might support transcription in growing oocytes. This hypothesis was indirectly confirmed by the experimental evidence that the content of labile zinc in the oocyte decreases when a major drop in transcription occurs in vivo. Accordingly, we observed that zinc sequestration with a zinc chelator rapidly reduced global transcription in growing oocytes, which was reversed by zinc supplementation in the culture medium. Finally, zinc supplementation impacted the chromatin state by reducing the level of global DNA methylation, which is consistent with the increased transcription. In conclusion, our study suggests that altering zinc availability by culture-medium supplementation supports global transcription, ultimately enhancing meiotic competence.

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Section: Discussionmentioning

confidence: 94%

Zinc supports transcription and improves meiotic competence of growing bovine oocytes

et al. 2020

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“…Different from BOSs, culture in PDHV yielded also a better follicle progression in HOSs. The different culture outcome in the two species could possibly be due to variations in tissue anatomy, metabolism and resistance to oxygen diffusion, and underlies that, in spite of the fact that bovine tissue is considered a reliable model for human in vitro folliculogenesis [ 38 ], it may behave differently from human tissue at least in some respects.…”

Section: Discussionmentioning

confidence: 99%

Is oxygen availability a limiting factor for in vitro folliculogenesis?

et al. 2018

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Transplantation of ovarian tissue for the preservation of fertility in oncological patients is becoming an accepted clinical practice. However, the risk of re-introducing tumour cells at transplantation has stirred an increased interest for complete in vitro folliculogenesis. This has not yet been achieved in humans possibly for the lack of knowledge on the environmental milieu that orchestrates folliculogenesis in vivo. The main aim of this study was to investigate the effect of oxygen availability on follicle health and growth during in vitro culture of ovarian tissue strips. To this end, a model was developed to predict the dissolved oxygen concentration in tissue under varying culture conditions. Ovarian cortical strips of bovine, adopted as an animal model, and human tissue were cultured in conventional (CD) and gas permeable (PD) dishes under different media column heights and gaseous oxygen tensions for 3, 6 and 9 days. Follicle quality, activation of primordial follicles to the primary stage, and progression to the secondary stage were analysed through histology. Follicle viability was assessed through a live-dead assay at the confocal scanning laser microscope. Findings showed a higher follicle quality and viability after culture of bovine ovarian strips in PD in adequate medium height and oxygen tensions. The best culture conditions found in the bovine were adopted for human ovarian strip culture and promoted a higher follicle quality, viability and progression. Overall, data demonstrated that modulation of oxygen availability in tissue plays a key role in maintaining follicles’ health and their ability to survive and progress to the secondary stage during ovarian tissue in vitro culture. Such culture conditions could increase the yield of healthy secondary follicles for subsequent dissection and individual culture to obtain competent oocytes.

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“…Similarly, the implementation of nanotechnology, which has already been developed for non-human animals, could be applied to assisted reproduction in humans ( Langbeen et al, 2015 ; Barkalina et al, 2016 ). As mentioned previously, this technology has already been tested and used in sectors adjacent to reproductive medicine.…”

Section: Potential Contributions Of Nanotechnology To Assisted Reprodmentioning

confidence: 99%

The Potential of Nanotechnology in Medically Assisted Reproduction

et al. 2018

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Reproductive medicine is a field of science which searches for new alternatives not only to help couples achieve pregnancy and preserve fertility, but also to diagnose and treat diseases which can impair the normal operation of the reproductive tract. Assisted reproductive technology (ART) is a set of methodologies applied to cases related to infertility. Despite being highly practiced worldwide, ART presents some challenges, which still require special attention. Nanotechnology, as a tool for reproductive medicine, has been considered to help overcome some of those impairments. Over recent years, nanotechnology approaches applied to reproductive medicine have provided strategies to improve diagnosis and increase specificity and sensitivity. For in vitro embryo production, studies in non-human models have been used to deliver molecules to gametes and embryos. The exploration of nanotechnology for ART would bring great advances. In this way, experiments in non-human models to test the development and safety of new protocols using nanomaterials are very important for informing potential future employment in humans. This paper presents recent developments in nanotechnology regarding impairments still faced by ART: ovary stimulation, multiple pregnancy, and genetic disorders. New perspectives for further use of nanotechnology in reproductive medicine studies are also discussed.

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Bovine in vitro reproduction models can contribute to the development of (female) fertility preservation strategies

Cited by 34 publications

References 199 publications

Zinc supports transcription and improves meiotic competence of growing bovine oocytes

Zinc supports transcription and improves meiotic competence of growing bovine oocytes

Is oxygen availability a limiting factor for in vitro folliculogenesis?

The Potential of Nanotechnology in Medically Assisted Reproduction

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