Bovine herpesvirus 6 in buffaloes (Bubalus bulalis) from the Amazon region, Brazil

Oliveira, Cairo Henrique Sousa de; Oliveira, Fernanda Gonçalves de; Gasparini, Marcela Ribeiro; Galinari, Grazielle Cossenzo Florentino; Lima, Graciela Kunrath; Fonseca, Antônio Augusto; Barbosa, José Diomedes; Barbosa-Stancioli, Edel Figueiredo; Leite, Rômulo Cerqueira; Reis, Jenner Karlisson Pimenta dos

doi:10.1007/s11250-014-0733-z

Cited by 17 publications

(28 citation statements)

References 11 publications

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“…BoHV6 has been detected in cattle as well as water buffaloes suffering from various diseases, such as lymphoproliferative disease, abortion, and postpartum metritis. 3,9,14,31 However, neither for this bovine lymphotropic herpesvirus, nor for the unclassified lymphotropic gammaherpesvirus found in small ruminants, 24,25 has a causative association to a disease been firmly established, and was not seen in our study either. Interestingly, even though BoHV6 was detected by panherpesvirus nPCR in one water buffalo, no NGS reads matched this virus.…”

Section: Discussioncontrasting

confidence: 75%

Viral infections shared between water buffaloes and small ruminants in Switzerland

et al. 2021

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Importation of exotic animals that may harbor infectious agents poses risks for native species with potentially severe impacts on animal health and animal production. Although the Asian water buffalo ( Bubalus bubalis) population in Europe is steadily increasing, its susceptibility to viral infections and its role for interspecies transmission is largely unknown. To identify viral infections that are shared between exotic water buffaloes and native small ruminants, we collected blood samples from 3 Swiss farms on which water buffaloes were kept either without, or together with, sheep or goats. These samples were analyzed by next-generation sequencing (NGS) as well as by selected conventional tests, including PCR, ELISA, and in some cases a virus neutralization test. By NGS, a novel virus of the genus Gemykrogvirus (GyKV; Genomoviridae) was first detected in the buffaloes on one farm, and subsequently confirmed by PCR, and was also detected in the co-housed sheep. In contrast, this virus was not detected in buffaloes on the farms without sheep. Moreover, conventional methods identified a number of viral infections that were not shared between the exotic and the native animals, and provided evidence for potential roles of water buffaloes in the epidemiology of ruminant pestiviruses, especially bovine viral diarrhea virus, bluetongue virus, and possibly bovine alphaherpesvirus 2. Our results clearly indicate that water buffaloes are susceptible to interspecies viral transmission and may act as intermediate hosts, or even as reservoirs, for these viruses.

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Section: Discussioncontrasting

confidence: 75%

Viral infections shared between water buffaloes and small ruminants in Switzerland

et al. 2021

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“…In humans, Epstein-Barr virus (EBV) is associated with the occurrence of Hodgkin lymphoma, Burkitt’s lymphoma, and diffuse lymphoma of large B cells, among others [ 23 ]. BoHV-6, a gammaherpesvirus initially detected in samples of bovine lymphomas caused by BLV [ 24 ], was not detected in samples of lymphoma from buffaloes evaluated in our study, despite its previous detection in the peripheral blood of other buffaloes from the same herd [ 10 ].…”

Section: Discussionmentioning

confidence: 55%

“…Real-time PCR was performed (qPCR) for BLV to detect the LTR gene, amplifying a 120-bp fragment according to a previous study [ 14 ]. Lastly, BoHV-6 was investigated by means of seminested PCR for the pol gene [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…Our group has detected the presence of some viruses infections in buffaloes in Brazil including Bovine herpesvirus 6 (BoHV-6) but no causal link to any diseases has so far been established [ 10 ]. Bovine immunodeficiency virus (BIV) was studied in 607 samples of DNA taken from the whole blood of buffaloes in Brazil and was detected in 4.4 % of cases using molecular biology techniques [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Multicentric lymphoma in buffaloes in the Amazon region, Brazil

et al. 2016

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BackgroundThe presence of lymphoma in buffaloes was first reported in India in the 1960s. The disease is similar to Enzootic Bovine Leucosis (EBL) caused by Bovine leukemia virus (BLV) in cattle; however, according to our results and those of other studies, the etiology of these lymphomas in buffalo do not appear to be associated with BLV. The objectives of this study are to describe four cases of the disease in buffaloes belonging to the same herd in the Amazon region of Brazil and to perform a clinical-anatomopathological, immunohistochemical, and etiological study of the lymphomas.ResultsOver a period of ten years, four buffaloes were observed presenting progressive weight loss, swelling of peripheral lymph nodes, and nodules in the subcutaneous tissue. Upon necropsy, whitish-colored tumor masses were observed in the form of nodules in the subcutaneous tissue, along with miliary nodules on the serosal surfaces of abdominal and thoracic organs and tumors in lymph nodes and other organs. Neoplastic lymphocyte proliferation was observed through histopathology. An immunohistochemical study revealed that the neoplasias were formed by proliferation of predominantly B lymphocytes. The presence of BLV genome was not detected in the lymphomas when using the real-time PCR technique, nor was it detected through immunohistochemical staining using monoclonal antibodies against two viral proteins. Bovine herpesvirus 6 was not detected in the tumors. However, Bovine immunodeficiency virus (BIV) was detected in samples of lymphoma and in the lymph nodes and kidneys of one of the animals.ConclusionsThe occurrence of lymphoma in buffaloes is reported for the first time in Brazil and is characterized by B-cell multicentric lymphoma. The etiology of the disease does not appear to be associated with BLV; however, the detection of BIV in samples of lymphoma from one sick animal deserves further study, considering the oncogenic potential of this virus.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0845-y) contains supplementary material, which is available to authorized users.

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“…Alphaherpesvírus bovino 1 (BoHV-1) apresenta caráter cosmopolita e está associado a uma gama de alterações sistêmicas, tais como: Rinotraqueíte Infecciosa Bovina -IBR, doença sistêmica em bezerros neonatos, Vulvovaginite e Balanopostite Infecciosa Bovina -IPV e IBP e abortamentos [7,12,14]. BoHV-1 é um vírus com genoma DNA fita dupla linear, com nucleocapsídeo icosaédrico e possui envelope lipoproteico [16].…”

Section: Introductionunclassified

Seroepidemiology Survey of Bovine Alphaherpesvirus 1 (BoHV-1) in Unvaccinated Beef Bubalines (Bubalus bubalis) from Southern Brazil

Oliveira

Fritzen

Alfieri

et al. 2021

Acta Scientiae. Vet.

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Background: The bovine alphaherpesvirus type 1 (BoHV-1) belonging to the order Herpesvirales, family Herpesviridae, subfamily Alphaherpesvirinae and genus Varicellovirus.Bovine alphaherpesvirus 1 (BoHV-1) infections have a great importance due to the high rates of dissemination in cattles worldwide. Although, the BoHV-1 was largely related in cattle, buffaloes were also classified as host for the virus. However, studies that determine seroepidemiological data in this species are scarce and necessary. The aim of the present study was to determine the presence of antibodies against BoHV-1 in healthy beef buffaloes using the virus neutralization (VN) technique.Cases: This work portrays an epidemiological survey, based on a sectional study characterized by blood samples collected from 54 Murrah buffalo, aged 6 to 24 months, from the municipalities of Guaraqueçaba, Ponta Grossa, Antonina and Doutor Ulysses, located in Paraná state, being 20, 14, 10 and 10 samples from each location, respectively. Thirty-seven percent (20/54) of the samples were collected at slaughterhouse with registration at the Federal Inspection Service (SIF) of the Ministry of Agriculture, Livestock and Food Supply (MAPA), and 63% (34/54) at the buffalo's farms. The serum samples were collected in sterile tubes without anticoagulant in stored isothermal boxes, with serum separation and stored at -20ºC. The samples were sent to the Animal Virology Laboratory of Universidade Estadual de Londrina, UEL for serological analysis. Serological diagnosis using the virus neutralization (VN) technique was performed according the OIE manual of diagnostic tests and vaccines for terrestrial animals, using the BoHV-1 Los Angeles strain. Forth-five of the 54 samples (83.3%) evaluated generated titers of antibodies against BoHV-1 present in all evaluated herds, and the samples collected in herd from Antonina city were 100% positive, following by 80% in Guaraqueçaba and Doutor Ulysses city, and 78.6% in Ponta Grossa city. In relationship to the titration of anti-bovine herpesvirus 1 antibodies (BoHV-1), it was found that 23/45 (51.12%) of the samples had titers ≤ 16, 13/45 (28.88 %) with indexes between 32-64, and 9/45 (20%) with levels above 128.Discussion: The confirmation of the presence of antibodies against BoHV-1 in the buffalo samples tested in the present study proves the circulation of the agent in the studied species, with a homogeneous distribution of bovine alphaherpesvirus 1 in all the evaluated herds. There was high variability in antibody titers against BoHV-1 from all herds, and the occurrence of a high number of buffaloes reactive to BoHV-1 and with high antibody titers suggests a course of active or reactive infections in these animals. In only one of the herds, the age of the animals studied varied between 6 to 8 months. The fact that these animals are lactating raises the possibility of anti-BoHV-1 antibodies being acquired by breastfeeding. The results obtained allow to conclude, considering the fact that the animals are not vaccinated against BoHV-1, the presence of circulating antibodies is from a natural infection, and the positivity in all farms tested denotes the endemicity of the BoHV-1 infection in herds. To the best knowledge of the authors, this is the first study on the detection and measurement of antibodies against BoHV-1 in healthy and unvaccinated buffaloes in the state of Paraná, southern Brazil, showing a high frequency of seroreagent animals.

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Bovine herpesvirus 6 in buffaloes (Bubalus bulalis) from the Amazon region, Brazil

Cited by 17 publications

References 11 publications

Viral infections shared between water buffaloes and small ruminants in Switzerland

Viral infections shared between water buffaloes and small ruminants in Switzerland

Multicentric lymphoma in buffaloes in the Amazon region, Brazil

Seroepidemiology Survey of Bovine Alphaherpesvirus 1 (BoHV-1) in Unvaccinated Beef Bubalines (Bubalus bubalis) from Southern Brazil

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