Bovine germinal vesicle oocyte and cumulus cell proteomics

Memili, Erdoğan; Peddinti, Divyaswetha; Shack, Leslie A.; Nanduri, Bindu; McCarthy, Fiona M.; Sağırkaya, Hakan; Burgess, Shane C.

doi:10.1530/rep-06-0149

Cited by 54 publications

(47 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2 B and C). The GV oocyte is tightly connected with cumulus cells, and these proteins may play a critical role in gap and adhesion junctions during GV oocyte maturation (25).…”

Section: Identification Of Total Proteins Expressed In Gv and MII Oocmentioning

confidence: 99%

Proteome of mouse oocytes at different developmental stages

Wang

Kou

Jing

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

216

221

View full text Add to dashboard Cite

The mammalian oocyte possesses powerful reprogramming factors, which can reprogram terminally differentiated germ cells (sperm) or somatic cells within a few cell cycles. Although it has been suggested that use of oocyte-derived transcripts may enhance the generation of induced pluripotent stem cells, the reprogramming factors in oocytes are undetermined, and even the identified proteins composition of oocytes is very limited. In the present study, 7,000 mouse oocytes at different developmental stages, including the germinal vesicle stage, the metaphase II (MII) stage, and the fertilized oocytes (zygotes), were collected. We successfully identified 2,781 proteins present in germinal vesicle oocytes, 2,973 proteins in MII oocytes, and 2,082 proteins in zygotes through semiquantitative MS analysis. Furthermore, the results of the bioinformatics analysis indicated that different protein compositions are correlated with oocyte characteristics at different developmental stages. For example, specific transcription factors and chromatin remodeling factors are more abundant in MII oocytes, which may be crucial for the epigenetic reprogramming of sperm or somatic nuclei. These results provided important knowledge to better understand the molecular mechanisms in early development and may improve the generation of induced pluripotent stem cells.germinal vesicle | metaphase II | zygote | protein | reprogramming R eprogramming of patient-specific somatic cells into pluripotent stem cells has attracted wide scientific and public interest because of the great potential value in both research and therapy. Recent advances in induced pluripotent stem cell (iPSC) research have clearly indicated that a small number of transcription factors can reverse the cell fate of differentiated somatic cells; however, the reprogramming process remains slow, and the efficiency is low. Typically, 1% of cells are reprogrammed, but this process requires at least 7 d to 2 wk (1-8). In contrast, reprogramming during somatic cell nuclear transfer (SCNT) occurs within one or two cell cycles and often in a majority of embryos (9-14). The oocytederived transcripts that promote this more efficient reprogramming remain unidentified; however, it has been suggested that their inclusion with the four transcription factors (Oct4, Sox2, Klf4, and c-Myc) may increase the speed and efficiency of the reprogramming process (15). As a step to identification of these factors, this project sought to define the proteome of mouse oocytes at three stages of development, which will also provide us important information on the factors regulating developmental competence of oocytes.During mammalian oogenesis, the oocyte undergoes two cell cycle arrests at the dictyate or germinal vesicle (GV) stage and the metaphase II (MII) stage (16,17). MII oocytes have been widely used to reprogram somatic cell nuclei, because during normal reproduction, sperm and oocyte nuclei are reprogrammed by the MII oocyte to produce totipotent zygotes. By contrast, results from our previous nucl...

show abstract

“…2 B and C). The GV oocyte is tightly connected with cumulus cells, and these proteins may play a critical role in gap and adhesion junctions during GV oocyte maturation (25).…”

Section: Identification Of Total Proteins Expressed In Gv and MII Oocmentioning

confidence: 99%

Proteome of mouse oocytes at different developmental stages

Wang

Kou

Jing

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

216

221

View full text Add to dashboard Cite

show abstract

“…Nuclear maturation mainly involves germinal vesicle breakdown, typically elicited by the LH surge at ovulation, followed by chromosome segregation and, finally, extrusion of the first polar body [35,36]. Cytoplasmic maturation is more complex to define, but involves organelles that reorganize and store the mRNA, proteins, and transcription factors required for oocyte maturation, fertilization, and subsequent embryonic development [37].…”

Section: In Vitro Maturationmentioning

confidence: 99%

Embryo Cryopreservation in Breast Cancer Patients

Bedoschi

Oktay

2016

Gonadal Tissue Cryopreservation in Fertility Preservation

View full text Add to dashboard Cite

Breast cancer is the second most common type of cancer expected to occur in women in 2014, accounting for 29 % of incident cases among women. Approximately 11 % of all breast cancer cases occur in women <45 years. Most of those patients are likely to undergo adjuvant systemic chemotherapy with a significant impact on ovarian reserve and future reproductive potential. Embryo cryopreservation is the most established technique for fertility preservation. This procedure is an attractive strategy for fertility preservation in reproductive age breast cancer patients with available partner or willing to use donor semen. Some special considerations should be given to ovarian stimulation for fertility preservation in breast cancer patients. The development of ovarian stimulation protocols using aromatase inhibitor combined with gonadotropin appears to be effective and safe for breast cancer patients. In this chapter, we will appraise and summarize the current state of embryo cryopreservation in breast cancer patients in order to provide a better understanding of the efficacy and safety of this fertility preservation technique. Keywords Embryo cryopreservation • Breast cancer • Fertility preservation • Ovarian stimulation • Letrozole IntroductionBreast cancer is a major public health problem in the United States and lives up to large investments in research, prevention, early diagnosis, and treatment.Breast cancer is the second most common type of cancer expected to occur in women in 2014, accounting for 29 % (232,670) of incident cases among women [1]. The relatively stable incidence rates during the past 5 years for which there are data reflect improvements in cancer control and screening methods. At the same time, the detection of cancer at an early stage and the development of the treatment

show abstract

“…Recently, there have been several reports analyzing mammalian oocyte proteomics, including exploration of bovine, pig and mouse oocyte proteins. Recently, proteomics analysis was used to differentiate protein expression among the nuclear maturation, fertilization and early embryogenesis stages of oocytes [1][2][3][4][5][6]. This information will improve our overall understanding of oocyte meiosis and maturation.…”

Section: Introductionmentioning

confidence: 99%

Protein Profile Involved in Mammalian Oocyte Maturation, Fertilization and Early Embryogenesis (Pre-Implantation)

Turathum¹,

Sroyraya²

2017

Cell Dev Biol

View full text Add to dashboard Cite

Proteomic analysis of oocytes can help identify proteins that are involved in female meiotic maturation and early embryonic development. Many proteins with well-defined functions have been identified during oocyte maturation. High levels of MPF, MAPK, Mos and low levels of cAMP play an essential role in the resumption of meiosis I. Following germinal vesicle breakdown, chromosome condensation and spindle formation occurred at metaphase I by assembly of the meiotic apparatus, which includes the proteins NuMA, γ-tubulin and Polo-like kinase 1. The metaphase II arrest is a result of high levels of MPF and MAPK. Proteins involved in the stress response and redox regulation, including peroxiredoxin, GST and HSF1, are also necessary for protection against oxidative stress. During fertilization, the sperm-egg interaction requires egg surface proteins, oocyte zona pellucida, molecular chaperones, GPI-anchored proteins and CD9 to recognize sperm proteins and prevent polyspermy. Following gamete fusion, resumption and complete of meiosis II is induced by GTP and CaM kinase II activation, which inactivates MPF and activation of the anaphase promoting complex/cyclosome results in sister chromatid separation. Decondensation of the sperm head begins after zona penetration and GSH and NPM2 are necessary for male pronuclear formation. MAPK inactivation is required for pronuclear formation. At the cleavage stage, the maternal effect proteins PADI6, FLOPED and FILIA are essential for embryonic progression past the two-cell stage. After cell adhesion, cell junctions and the cytoskeleton play an important role in compaction of the morula. Par6, Par3 and protein kinase C are components of the apical polarity complex and are important for formation of the blastocoel cavity. During the blastocyst stage, TEAD4 and CDX2 are required for trophoectoderm formation. This proteomic analysis of oocytes has improved our understanding of the molecular processes that regulate oocyte maturation, fertilization and pre-implantation in mammals.

show abstract

Bovine germinal vesicle oocyte and cumulus cell proteomics

Cited by 54 publications

References 45 publications

Proteome of mouse oocytes at different developmental stages

Proteome of mouse oocytes at different developmental stages

Embryo Cryopreservation in Breast Cancer Patients

Protein Profile Involved in Mammalian Oocyte Maturation, Fertilization and Early Embryogenesis (Pre-Implantation)

Contact Info

Product

Resources

About