Bovine Fertility as Regulated by Sperm Binding Proteins: A Review

Dar, Mohammad Rayees; Singh, Mahendra; Sharma, R.; Thakur, S.S.; Shei, Aasif Ahmad; Bhat, Showkat Ahmad

doi:10.3923/ajava.2018.6.13

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…In bulls, the BSP proteins are a major class of proteins contained in SP. Such proteins are: PDC-109 (BSP-A1/-A2), BSP-A3 and BSP-30-kDa (Dar et al 2018). It is considered that some of these proteins bind to the sperm membrane phosphatidylcholine during ejaculation.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that some SPPs affect the motility and survival ability of the spermatozoa, thus influencing the sperm fertilization potential (Codognoto et al 2018). Various groups of SPPs have been associated with fertility in horses, bulls and humans (Battut et al 2005, Mogielnicka-Brzozowska 2011, Dar et al 2018. The biological role of certain SPPs in the sperm capacitation process has been defined (Kim et al 2015, Luna et al 2015, Arangasamy and Singh 2017).…”

Section: Introductionmentioning

confidence: 99%

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Polish Journal of Veterinary Sciences

Ivanova

Gradinarska

Tsvetkov

et al. 2018

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The aim of this study was to analyse and identify specific buffalo seminal plasma proteins (SPPs) responsible for sperm cryotolerance during low temperature storage. Computer Assisted Sperm Analysis (CASA) of the motility and viability of buffalo spermatozoa was performed before freezing and after thawing. Two sample groups were formed -ejaculates with high cryotolerance (group A) and low cryotolerance (group B). CASA demonstrated that the initial progressive motility after thawing of the spermatozoa in group A is significantly higher than in group B (p<0.001). Group B showed a significant increase in the percentage of static and non-progressive spermatozoa at 240 min, when compared to group A (p<0.05). SPPs, proteins in the cryoprotective medium (PM) and proteins in the mixture of PM and SP were separated by High Performance Liquid Chromatography (HPLC). Comparative analysis of the chromatographic profiles was performed to identify specific proteins related to sperm cryotolerance. SPPs profiles showed 5 distinct protein peaks in both groups, ranging from 500 kDa to 50 Da. Chromatograms of group A and group B showed quantitative and qualitative differences in protein content. Chromatograms of proteins in PM showed 11 well-expressed peaks. HPLC analysis of the mixtures of SPPs from the two groups and PM visualized the formation of a new bio-complex structure expressed by a protein peak specific for group A (7.674 min, AU 1.50). This protein peak can be referred as a phenotypic trait for buffalo ejaculates with high sperm cryotolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polish Journal of Veterinary Sciences

Ivanova

Gradinarska

Tsvetkov

et al. 2018

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“…It is believed that BSPs bind to the outer leaflet of sperm membranes during ejaculation. Besides playing important roles as mediators of capacitation [ 156 ], it has been reported that BSPs act as chaperones in sperm motility and viability and mediate sperm-egg interactions [ 157 ]. Moreover, BSPs have been implicated in sperm freezability due to their ability to bind to egg yolk low-density lipoproteins [ 158 ] and milk components [ 159 ] of semen extenders, thus protecting spermatozoa exposed to low temperatures from the loss of membrane lipids.…”

Section: Protein Markersmentioning

confidence: 99%

Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

Ďuračka

Benko

Tvrdá

2023

IJMS

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For decades now, sperm cryopreservation has been a pillar of assisted reproduction in animals as well as humans. Nevertheless, the success of cryopreservation varies across species, seasons, and latitudes and even within the same individual. With the dawn of progressive analytical techniques in the field of genomics, proteomics, and metabolomics, new options for a more accurate semen quality assessment have become available. This review summarizes currently available information on specific molecular characteristics of spermatozoa that could predict their cryotolerance before the freezing process. Understanding the changes in sperm biology as a result of their exposure to low temperatures may contribute to the development and implementation of appropriate measures to assure high post-thaw sperm quality. Furthermore, an early prediction of cryotolerance or cryosensitivity may lead to the establishment of customized protocols interconnecting adequate sperm processing procedures, freezing techniques, and cryosupplements that are most feasible for the individual needs of the ejaculate.

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“…In recent time, functional data analysis from proteomic studies have helped researchers to generate knowledge to better understand the relationship and mechanism underlying male fertility and sperm proteome (Rahman, et al, 2013;Ashok, et. al, 2016;Mohammad, et. al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Proteome of bull fertility: a comparative study between resting and in-service Nellore bulls

Udoekong

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This study aimed to analyses sperm proteome of Nellore bulls by comparing their sperm protein profiles and identifying differentially abundant proteins during their in-service and resting periods. A total of twenty bulls aged between 19.8 and 22.7 months were investigated at their resting period and at the breeding season (in-service). Semen samples were collected from bulls by electroejaculatation and preserved in liquid nitrogen. A total of 155 sperm proteins were identified from the protein analysis using SDA-PAGE. 84 of these proteins were specific to both resting bulls and in-service bulls while 61 were found only in resting bulls and five found only in the samples from in-service bulls. The differentially expressed sperm proteins observed in sodium dodecyl sulfate polyacrylamide gel electrophoresis were identified using LC-MS/MS. In resting bulls, albumin was differentially expressed and significantly (p<0.05) lower, down regulated in in-service bulls. SRN, bovin proacrosine, GAPDHS, ASRGL1, BSP1, CES5A, SPACA3, SPAM1, ADAM32 and ACE were significantly (p<0.05) lower and down regulated in in-service bulls. SPADH1 and LOC101907989 were differentially expressed in in-service bulls, significantly (p<0.05) lower and down regulated in resting bulls; SPADH1 (spermadhesin-1) associates with seminal plasma proteins BSP-30k Da, seminal plasma PDC-109, seminal plasma A3, ANG1, RNASE4, SRN and carbohydrate-binding proteins (uncharacterized protein). These findings suggest that these sperm proteins play significant role and are associated with increased fertility in bull during in-service periods rather than when they are at rest. This study suggests prior clean-up of breeding bulls before breeding season to allow for biological processes and favorable correlations of other sperm protein like SPADH1 in the regulation of sperm maturation, functions, fertility and oocyte-sperm interaction. Keywords: Fertility. Seminal plasma proteins. Spermadhesin-1.

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Bovine Fertility as Regulated by Sperm Binding Proteins: A Review

Cited by 4 publications

References 39 publications

Polish Journal of Veterinary Sciences

Polish Journal of Veterinary Sciences

Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

Proteome of bull fertility: a comparative study between resting and in-service Nellore bulls

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